AVALIAÇÃO DO EFEITO CICATRIZANTE DE FILMES DE ALGINATO CONTENDO GEL DE BABOSA - Aloe vera (L.) Burm. f.

Detalhes bibliográficos
Autor(a) principal: Koga, Adriana Yuriko
Data de Publicação: 2017
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UEPG
Texto Completo: http://tede2.uepg.br/jspui/handle/prefix/203
Resumo: Alginate is a polymer extracted from seaweed and traditionally used in various types of wound dressings. It is a polysaccharide which has bioadhesive and swelling characteristics. The ability of alginate to form gel and films provides a favorable environment for healing and re-epithelialization of the injured tissue. Aloe vera (L.) Burm. f., or simply Aloe, is a medicinal plant with analgesic, anti-inflammatory and healing properties. The purpose of this study was to develop an alginate film containing Aloe gel and examine its cutaneous healing effect in vivo. The film was obtained by casting of the aqueous sodium alginate solution containing glycerin and Aloe gel, followed by crosslinking with calcium chloride. The film was characterized by mechanical tensile strength, hydration degree, FTIR spectroscopy, scanning electron microscopy, polysaccharide and calcium release. The wound healing effect was studied by performing surgical wounds in the back of Wistar rats. The animals (n = 60) were divided into three groups: gauze dressings (control), dressing with alginate film and dressing with alginate-aloe film. The retraction of wounds was measured every 3 days. On days 3, 7, 14, 22 five animals from each group were euthanized and samples of skin were removed and fixed in formalin 10%. The samples for histological analysis were stained with hematoxylin-eosin and analyzed for quantification of inflammatory infiltrate, angiogenesis and macrophages. Collagen fibers were evaluated using a picrosirius red staining for differentiation of type I and III fibers. The alginate-aloe film exhibited desirable physical and mechanical characteristics for the wound dressing application. In vivo animal test revealed there was no significant difference among the three groups in wound retraction. The results of histological analysis showed a beneficial effects of alginate-aloe (p=0.04) and alginate films (p=0.05) decreasing the inflammatory infiltrate at 14th days, in relation to the control group. On the 7th day, there was an increased angiogenesis in the control group compared to the group treated with alginate-aloe films (p = 0.04) and alginate (p = 0.024). Treatment with alginate-aloe film increased the number of blood vessels on the 14th day, when compared to the control group (p = 0.004); in addition, it caused decrease of macrophages on day 22, when compared to the other groups (alginate film (p = 0,0) and control (p = 0.007). The evaluation of the collagen fibers showed a significant difference between the alginate-aloe film and control group, with evident decrease of type III fibers (p = 0.05) and increase of type I fibers (p = 0.024) between days 14 and 22, suggesting an improvement in the progression of the healing process.
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spelling Pereira, Airton VicenteCPF:13881344896http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763209E2Franco, Gilson Cesar NobreCPF:19703868860http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4129277Z3Ollhoff, Rudiger DanielCPF:18968049858http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4790026Y9CPF:39762156862http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4473317A5Koga, Adriana Yuriko2017-07-21T14:35:57Z2017-05-172017-07-21T14:35:57Z2017-02-23KOGA, Adriana Yuriko. Avaliação do efeito cicatrizante de filmes de alginato contendo gel de babosa Aloe vera (L.) Burmx. 2017. 71 f. Dissertação (Mestrado em Biologia Celular e Molecular, Fisiologia e Fisiopatologia) - UNIVERSIDADE ESTADUAL DE PONTA GROSSA, Ponta Grossa, 2017.http://tede2.uepg.br/jspui/handle/prefix/203Alginate is a polymer extracted from seaweed and traditionally used in various types of wound dressings. It is a polysaccharide which has bioadhesive and swelling characteristics. The ability of alginate to form gel and films provides a favorable environment for healing and re-epithelialization of the injured tissue. Aloe vera (L.) Burm. f., or simply Aloe, is a medicinal plant with analgesic, anti-inflammatory and healing properties. The purpose of this study was to develop an alginate film containing Aloe gel and examine its cutaneous healing effect in vivo. The film was obtained by casting of the aqueous sodium alginate solution containing glycerin and Aloe gel, followed by crosslinking with calcium chloride. The film was characterized by mechanical tensile strength, hydration degree, FTIR spectroscopy, scanning electron microscopy, polysaccharide and calcium release. The wound healing effect was studied by performing surgical wounds in the back of Wistar rats. The animals (n = 60) were divided into three groups: gauze dressings (control), dressing with alginate film and dressing with alginate-aloe film. The retraction of wounds was measured every 3 days. On days 3, 7, 14, 22 five animals from each group were euthanized and samples of skin were removed and fixed in formalin 10%. The samples for histological analysis were stained with hematoxylin-eosin and analyzed for quantification of inflammatory infiltrate, angiogenesis and macrophages. Collagen fibers were evaluated using a picrosirius red staining for differentiation of type I and III fibers. The alginate-aloe film exhibited desirable physical and mechanical characteristics for the wound dressing application. In vivo animal test revealed there was no significant difference among the three groups in wound retraction. The results of histological analysis showed a beneficial effects of alginate-aloe (p=0.04) and alginate films (p=0.05) decreasing the inflammatory infiltrate at 14th days, in relation to the control group. On the 7th day, there was an increased angiogenesis in the control group compared to the group treated with alginate-aloe films (p = 0.04) and alginate (p = 0.024). Treatment with alginate-aloe film increased the number of blood vessels on the 14th day, when compared to the control group (p = 0.004); in addition, it caused decrease of macrophages on day 22, when compared to the other groups (alginate film (p = 0,0) and control (p = 0.007). The evaluation of the collagen fibers showed a significant difference between the alginate-aloe film and control group, with evident decrease of type III fibers (p = 0.05) and increase of type I fibers (p = 0.024) between days 14 and 22, suggesting an improvement in the progression of the healing process.O alginato é um polímero extraído de algas marinhas e tradicionalmente utilizado na fabricação de curativos. A propriedade de formar géis e filmes faz com que o alginato proporcione um ambiente favorável à cicatrização e a re-epitelização do tecido lesado. Aloe vera (L.) Burm. f., popularmente conhecida como babosa, é uma planta medicinal que apresenta propriedades analgésica, anti-inflamatória e cicatrizante. O objetivo deste trabalho foi desenvolver um filme de alginato contendo gel de babosa e avaliar o seu efeito cicatrizante in vivo. O filme foi obtido por evaporação de uma solução aquosa de alginato de sódio contendo glicerina, gel de babosa e reticulado com cloreto de cálcio. O filme foi caracterizado por ensaios de resistência mecânica a tração, hidratação, espectroscopia no infravermelho (FTIR), microscopia eletrônica de varredura e liberação de polissacarídeos e íons cálcio. Na avaliação do efeito cicatrizante, foi utilizado o modelo de lesão cirúrgica na pele da região cérvico-lombar de ratos Wistar. Os animais (n=60) foram divididos em três grupos: curativos com gaze (controle), curativos com filme de alginato e curativos com filme alginato-babosa. As medidas da retração das feridas foram realizadas a cada 3 dias. Nos dias 3, 7, 14 e 22 cinco animais de cada grupo foram eutanasiados e um fragmento de pele foi retirado e fixado em formol 10%. As análises histológicas dos tecidos foram realizadas utilizando-se coloração hematoxilina eosina para quantificação de infiltrado inflamatório, avaliação da angiogênese e contagem de macrófagos. As fibras colágenas foram avaliadas utilizando a coloração picrosirius red para diferenciação das fibras de tipo I e III. As análises físico-químicas do filme evidenciaram características ideais para a aplicação como curativos. No ensaio do efeito cicatrizante, não houve diferença significativa entre os grupos na avaliação da retração da lesão. Os resultados das análises histológicas mostraram uma diminuição do infiltrado inflamatório com os filmes alginato-babosa (p=0,04) e alginato (p=0,05) no 140 dia, em relação ao grupo controle. No 70 dia, observou-se uma maior angiogênese no grupo controle comparado aos grupos filme alginato-babosa (p=0,04) e alginato (p=0,024). O tratamento com o filme alginato-babosa aumentou o número de vasos sanguíneos no dia 14, quando comparado ao grupo controle (p=0,004); além disso, ocasionou uma diminuição mais expressiva de macrófagos no dia 22, quando comparado aos demais grupos (filme alginato (p=0,0) e controle (p=0,007)). A avaliação das fibras colágenas mostrou uma diferença significativa entre os grupos filme alginato-babosa e controle, com evidente diminuição das fibras tipo III (p=0,05) e aumento das fibras tipo I (p=0,024) entre os dias 14 e 22, sugerindo uma melhora na progressão do processo de cicatrização.Made available in DSpace on 2017-07-21T14:35:57Z (GMT). No. of bitstreams: 1 ADRIANA YURIKO KOGA.pdf: 2085290 bytes, checksum: 04ba643fa625913ec2b768408faac0ab (MD5) Previous issue date: 2017-02-23application/pdfporUNIVERSIDADE ESTADUAL DE PONTA GROSSAPrograma de Pós Graduação em Ciências BiomédicasUEPGBRBiologia Celular e Molecular, Fisiologia e Fisiopatologiaalginatofilme babosacurativocicatrizaçãoalginatefilmAloe veradressinghealingCNPQ::CIENCIAS DA SAUDE::MEDICINAAVALIAÇÃO DO EFEITO CICATRIZANTE DE FILMES DE ALGINATO CONTENDO GEL DE BABOSA - Aloe vera (L.) Burm. f.Avaliação do efeito cicatrizante de filmes de alginato contendo gel de babosa Aloe vera (L.) 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dc.title.por.fl_str_mv AVALIAÇÃO DO EFEITO CICATRIZANTE DE FILMES DE ALGINATO CONTENDO GEL DE BABOSA - Aloe vera (L.) Burm. f.
dc.title.alternative.eng.fl_str_mv Avaliação do efeito cicatrizante de filmes de alginato contendo gel de babosa Aloe vera (L.) Burmx
title AVALIAÇÃO DO EFEITO CICATRIZANTE DE FILMES DE ALGINATO CONTENDO GEL DE BABOSA - Aloe vera (L.) Burm. f.
spellingShingle AVALIAÇÃO DO EFEITO CICATRIZANTE DE FILMES DE ALGINATO CONTENDO GEL DE BABOSA - Aloe vera (L.) Burm. f.
Koga, Adriana Yuriko
alginato
filme babosa
curativo
cicatrização
alginate
film
Aloe vera
dressing
healing
CNPQ::CIENCIAS DA SAUDE::MEDICINA
title_short AVALIAÇÃO DO EFEITO CICATRIZANTE DE FILMES DE ALGINATO CONTENDO GEL DE BABOSA - Aloe vera (L.) Burm. f.
title_full AVALIAÇÃO DO EFEITO CICATRIZANTE DE FILMES DE ALGINATO CONTENDO GEL DE BABOSA - Aloe vera (L.) Burm. f.
title_fullStr AVALIAÇÃO DO EFEITO CICATRIZANTE DE FILMES DE ALGINATO CONTENDO GEL DE BABOSA - Aloe vera (L.) Burm. f.
title_full_unstemmed AVALIAÇÃO DO EFEITO CICATRIZANTE DE FILMES DE ALGINATO CONTENDO GEL DE BABOSA - Aloe vera (L.) Burm. f.
title_sort AVALIAÇÃO DO EFEITO CICATRIZANTE DE FILMES DE ALGINATO CONTENDO GEL DE BABOSA - Aloe vera (L.) Burm. f.
author Koga, Adriana Yuriko
author_facet Koga, Adriana Yuriko
author_role author
dc.contributor.advisor1.fl_str_mv Pereira, Airton Vicente
dc.contributor.advisor1ID.fl_str_mv CPF:13881344896
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4763209E2
dc.contributor.referee1.fl_str_mv Franco, Gilson Cesar Nobre
dc.contributor.referee1ID.fl_str_mv CPF:19703868860
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4129277Z3
dc.contributor.referee2.fl_str_mv Ollhoff, Rudiger Daniel
dc.contributor.referee2ID.fl_str_mv CPF:18968049858
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4790026Y9
dc.contributor.authorID.fl_str_mv CPF:39762156862
dc.contributor.authorLattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4473317A5
dc.contributor.author.fl_str_mv Koga, Adriana Yuriko
contributor_str_mv Pereira, Airton Vicente
Franco, Gilson Cesar Nobre
Ollhoff, Rudiger Daniel
dc.subject.por.fl_str_mv alginato
filme babosa
curativo
cicatrização
topic alginato
filme babosa
curativo
cicatrização
alginate
film
Aloe vera
dressing
healing
CNPQ::CIENCIAS DA SAUDE::MEDICINA
dc.subject.eng.fl_str_mv alginate
film
Aloe vera
dressing
healing
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS DA SAUDE::MEDICINA
description Alginate is a polymer extracted from seaweed and traditionally used in various types of wound dressings. It is a polysaccharide which has bioadhesive and swelling characteristics. The ability of alginate to form gel and films provides a favorable environment for healing and re-epithelialization of the injured tissue. Aloe vera (L.) Burm. f., or simply Aloe, is a medicinal plant with analgesic, anti-inflammatory and healing properties. The purpose of this study was to develop an alginate film containing Aloe gel and examine its cutaneous healing effect in vivo. The film was obtained by casting of the aqueous sodium alginate solution containing glycerin and Aloe gel, followed by crosslinking with calcium chloride. The film was characterized by mechanical tensile strength, hydration degree, FTIR spectroscopy, scanning electron microscopy, polysaccharide and calcium release. The wound healing effect was studied by performing surgical wounds in the back of Wistar rats. The animals (n = 60) were divided into three groups: gauze dressings (control), dressing with alginate film and dressing with alginate-aloe film. The retraction of wounds was measured every 3 days. On days 3, 7, 14, 22 five animals from each group were euthanized and samples of skin were removed and fixed in formalin 10%. The samples for histological analysis were stained with hematoxylin-eosin and analyzed for quantification of inflammatory infiltrate, angiogenesis and macrophages. Collagen fibers were evaluated using a picrosirius red staining for differentiation of type I and III fibers. The alginate-aloe film exhibited desirable physical and mechanical characteristics for the wound dressing application. In vivo animal test revealed there was no significant difference among the three groups in wound retraction. The results of histological analysis showed a beneficial effects of alginate-aloe (p=0.04) and alginate films (p=0.05) decreasing the inflammatory infiltrate at 14th days, in relation to the control group. On the 7th day, there was an increased angiogenesis in the control group compared to the group treated with alginate-aloe films (p = 0.04) and alginate (p = 0.024). Treatment with alginate-aloe film increased the number of blood vessels on the 14th day, when compared to the control group (p = 0.004); in addition, it caused decrease of macrophages on day 22, when compared to the other groups (alginate film (p = 0,0) and control (p = 0.007). The evaluation of the collagen fibers showed a significant difference between the alginate-aloe film and control group, with evident decrease of type III fibers (p = 0.05) and increase of type I fibers (p = 0.024) between days 14 and 22, suggesting an improvement in the progression of the healing process.
publishDate 2017
dc.date.accessioned.fl_str_mv 2017-07-21T14:35:57Z
dc.date.available.fl_str_mv 2017-05-17
2017-07-21T14:35:57Z
dc.date.issued.fl_str_mv 2017-02-23
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dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv KOGA, Adriana Yuriko. Avaliação do efeito cicatrizante de filmes de alginato contendo gel de babosa Aloe vera (L.) Burmx. 2017. 71 f. Dissertação (Mestrado em Biologia Celular e Molecular, Fisiologia e Fisiopatologia) - UNIVERSIDADE ESTADUAL DE PONTA GROSSA, Ponta Grossa, 2017.
dc.identifier.uri.fl_str_mv http://tede2.uepg.br/jspui/handle/prefix/203
identifier_str_mv KOGA, Adriana Yuriko. Avaliação do efeito cicatrizante de filmes de alginato contendo gel de babosa Aloe vera (L.) Burmx. 2017. 71 f. Dissertação (Mestrado em Biologia Celular e Molecular, Fisiologia e Fisiopatologia) - UNIVERSIDADE ESTADUAL DE PONTA GROSSA, Ponta Grossa, 2017.
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