ESTUDO QUÍMICO E BIOATIVIDADES DE Baccharis uncinella DC.

Detalhes bibliográficos
Autor(a) principal: Ascari, Jociani
Data de Publicação: 2007
Tipo de documento: Dissertação
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UEPG
Texto Completo: http://tede2.uepg.br/jspui/handle/prefix/2120
Resumo: Baccharis uncinella (Asteraceae), popularly known as vassoura, is little known from the chemical point of view, with only two earlier scientific works on their secondary metabolites - the volatile constituents. In the present study, the essential oil from the leaves of B. uncinella and nine extracts obtained in a Soxhlet apparatus with organic solvents starting from the leaves, barks and log were analyzed by CCD, UV and IV and submitted to activity tests against microorganisms; besides, the interactions between the aqueous extract of the leaves with human erytrocytary cells of the sanguine types O+, O-, A+ and B+ were also analyzed. In the antimicrobial tests, the microfungus Candida albicans, as well as the Gram-positive Bacillus cereus, Enterococcus faecalis, Staphylococcus aureus and S. saprophyticus and the Gram-negative bacteria Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa were used. The essential oil showed action against B. cereus, S. aureus and C. albicans, in the same way as all of the organic extracts, but the growth of S. saprophyticus was not inhibited by no one of the extracts. The extracts of the barks, when compared with those obtained from other parts of the plant, were active against the largest number of microorganisms and presented the more strong activities. The minimum inhibitory concentration of the essential oil was estimated as 100/mL against S. aureus as well as against C. albicans, wich could be considered a moderate antimicrobial activity. We have isolated small amounts of the three major phenolic components found in the leaves, and the analysis of their UV- and IR-spectra showed the structural features of hydroxylaryl-propenoic acids for these compounds that were found for the first time in this plant species. The analyses of GC-MS of the essential oil produced by hydrodistillation of the leaves collected at the Paraná State presented low monoterpene content (2.29%) and the following sesquiterpenes as the main components: spathulenol 17.20%, caryophyllene oxide 16.20%, transcaryophyllene 10.48%, eudesmol 7.55%, germacrene D 6.28% and cadinol 5.18%. Another oil sample was obtained using the same conditions, but starting with leaves collected in the highland area of Santa Catarina, also presented spathulenol as the main component (15.83%), but it contained 36% in monoterpenes, a very similar composition to those published for samples from the State of Rio Grande do Sul. The interactions among the aqueous extract of the leaves with human erytrocytes isolated from the sanguine types O+, O-, A+ and B+, being verified that the extract presents a non-lytic activity in the analyzed concentrations of 22, 110 and 220/mL. This non-hemolytic profile was observed as similar for all tested blood types. The serological types A+ and O- presented a similar pattern of free Hb oxidation in solution, and the same profile was observed in the new suspension of the precipitate. In the analysis of MEV we observed the qualitative aspects regarding the interaction of the aqueous extract with the architecture of the erythrocyte. In the assays of DPPH with low concentrations, 1.5 x 10-4 and 7.8 x 10-5 mg/mL, it was possible to quantify a partial reduction in the free radicals concentration of 20.10% and 9.25% respectively.
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spelling Nunes, Domingos SávioCPF:24650560934http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781201Y6Marques, Mariza BoscacciCPF:26387247004http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4727372D5Cechinel Filho, ValdirCPF:44347600925http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784252A2Arrúa, Maria Elena PayretCPF:49793144068http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780152D1CPF:04006705930http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4137662H6Ascari, Jociani2017-07-24T19:38:11Z2007-07-302017-07-24T19:38:11Z2007-02-28ASCARI, Jociani. ESTUDO QUÍMICO E BIOATIVIDADES DE Baccharis uncinella DC.. 2007. 78 f. Dissertação (Mestrado em Química) - UNIVERSIDADE ESTADUAL DE PONTA GROSSA, Ponta Grossa, 2007.http://tede2.uepg.br/jspui/handle/prefix/2120Baccharis uncinella (Asteraceae), popularly known as vassoura, is little known from the chemical point of view, with only two earlier scientific works on their secondary metabolites - the volatile constituents. In the present study, the essential oil from the leaves of B. uncinella and nine extracts obtained in a Soxhlet apparatus with organic solvents starting from the leaves, barks and log were analyzed by CCD, UV and IV and submitted to activity tests against microorganisms; besides, the interactions between the aqueous extract of the leaves with human erytrocytary cells of the sanguine types O+, O-, A+ and B+ were also analyzed. In the antimicrobial tests, the microfungus Candida albicans, as well as the Gram-positive Bacillus cereus, Enterococcus faecalis, Staphylococcus aureus and S. saprophyticus and the Gram-negative bacteria Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa were used. The essential oil showed action against B. cereus, S. aureus and C. albicans, in the same way as all of the organic extracts, but the growth of S. saprophyticus was not inhibited by no one of the extracts. The extracts of the barks, when compared with those obtained from other parts of the plant, were active against the largest number of microorganisms and presented the more strong activities. The minimum inhibitory concentration of the essential oil was estimated as 100/mL against S. aureus as well as against C. albicans, wich could be considered a moderate antimicrobial activity. We have isolated small amounts of the three major phenolic components found in the leaves, and the analysis of their UV- and IR-spectra showed the structural features of hydroxylaryl-propenoic acids for these compounds that were found for the first time in this plant species. The analyses of GC-MS of the essential oil produced by hydrodistillation of the leaves collected at the Paraná State presented low monoterpene content (2.29%) and the following sesquiterpenes as the main components: spathulenol 17.20%, caryophyllene oxide 16.20%, transcaryophyllene 10.48%, eudesmol 7.55%, germacrene D 6.28% and cadinol 5.18%. Another oil sample was obtained using the same conditions, but starting with leaves collected in the highland area of Santa Catarina, also presented spathulenol as the main component (15.83%), but it contained 36% in monoterpenes, a very similar composition to those published for samples from the State of Rio Grande do Sul. The interactions among the aqueous extract of the leaves with human erytrocytes isolated from the sanguine types O+, O-, A+ and B+, being verified that the extract presents a non-lytic activity in the analyzed concentrations of 22, 110 and 220/mL. This non-hemolytic profile was observed as similar for all tested blood types. The serological types A+ and O- presented a similar pattern of free Hb oxidation in solution, and the same profile was observed in the new suspension of the precipitate. In the analysis of MEV we observed the qualitative aspects regarding the interaction of the aqueous extract with the architecture of the erythrocyte. In the assays of DPPH with low concentrations, 1.5 x 10-4 and 7.8 x 10-5 mg/mL, it was possible to quantify a partial reduction in the free radicals concentration of 20.10% and 9.25% respectively.Baccharis uncinella (Asteraceae), popularmente conhecida como vassoura, é pouco conhecida do ponto de vista químico, havendo apenas dois trabalhos científicos anteriores sobre seus metabólitos secundários - os constituintes voláteis. No presente estudo, o óleo essencial das folhas de B.uncinella e nove extratos obtidos em Soxhlet com solventes orgânicos a partir das folhas, cascas e lenho foram analisados por CCD, UV e IV e submetidos a testes de atividade contra microrganismos; além disto, foram também analisadas as interações de extrato aquoso das folhas com células eritrocitárias humanas dos tipos sangüíneos O+, O-, A+ e B+. Nos testes antimicrobianos, foram usados o microfungo Candida albicans, as bactérias Gram-positivas Bacillus cereus, Enterococcus faecalis, Staphylococcus aureus e S. saprophyticus e as bactérias Gram-negativas Escherichia coli, Klebsiella pneumoniae e Pseudomonas aeruginosa. O óleo essencial apresentou ação contra B. cereus, S. aureus e C. albicans, da mesma forma que todos os extratos orgânicos, mas o crescimento de S. saprophyticus não foi inibido por nenhum deles. Os extratos das cascas, quando comparados com os obtidos de outras partes da planta, foram ativos contra o maior número de microrganismos e apresentaram as mais fortes atividades. A concentração inibitória mínima do óleo essencial foi determinada como 50L/mL tanto contra S. aureus como contra C. albicans, podendo ser considerada uma atividade antimicrobiana moderada. Foram isoladas pequenas quantidades dos três compostos fenólicos majoritários, cujas análises indicam trata-se de derivados de ácidos hidroxiarilpropenóicos, encontrados pela primeira vez nesta espécie vegetal. As análises de CG-EM do óleo essencial produzido por hidrodestilação a partir de folhas coletadas no segundo planalto do Paraná, apresentaram baixo teor de monoterpenos (2,29%) e os seguintes sesquiterpenos como principais componentes: espatulenol 17,20%, cariofileno óxido 16,20%, trans-cariofileno 10,48%, eudesmol 7,55%, germacreno D 6,28% e cadinol 5,18%. Outra amostra de óleo obtida nas mesmas condições, mas a partir de folhas coletadas na região serrana de Santa Catarina, também apresentou espatulenol como principal componente (15,83%), mas quase 36% em monoterpenos, uma composição muito similar às publicadas sobre amostras da Serra Gaúcha. Foram analisadas as interações entre extrato aquoso das folhas com células eritrocitárias humanas dos tipos sangüíneos O+, O-, A+ e B+, sendo que o extrato apresenta uma atividade não-lítica nas concentrações analisadas de 22, 110 e 220 /mL, similar para todas tipagens analisadas. Os sorotipos A+ e O- testados apresentaram um padrão similar de oxidação da Hb livre em solução e, o mesmo perfil foi observado na ressuspensão dos precipitados. Na análise de MEV foram verificados aspectos qualitativos a respeito da interação do extrato aquoso com a arquitetura da célula eritrocitária. Baixas concentrações do extrato, 1,5 x 10-4 e 7,8 x 10-5 mg/mL, apresentaram atividade redutora em presença de DPPH, de 20,10% e 9,25% respectivamente.Made available in DSpace on 2017-07-24T19:38:11Z (GMT). No. of bitstreams: 1 Jociani Ascari.pdf: 1347077 bytes, checksum: 5bf88ebdcff72a3eee1c4ed2152eb55c (MD5) Previous issue date: 2007-02-28Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUNIVERSIDADE ESTADUAL DE PONTA GROSSAPrograma de Pós-Graduação em Química AplicadaUEPGBRQuímicaBaccharis uncinellacomposição químicaatividade antimicrobianaóleo essencialextratos orgânicosbioatividadehemóliseantioxidanteBaccharis uncinellachemical compositionantimicrobial activityessential oilsolvent extractsbioactivityhemolysis antioxidantCNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICAESTUDO QUÍMICO E BIOATIVIDADES DE Baccharis uncinella DC.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEPGinstname:Universidade Estadual de Ponta Grossa (UEPG)instacron:UEPGORIGINALJociani Ascari.pdfapplication/pdf1347077http://tede2.uepg.br/jspui/bitstream/prefix/2120/1/Jociani%20Ascari.pdf5bf88ebdcff72a3eee1c4ed2152eb55cMD51prefix/21202017-07-24 16:38:11.599oai:tede2.uepg.br:prefix/2120Biblioteca Digital de Teses e Dissertaçõeshttps://tede2.uepg.br/jspui/PUBhttp://tede2.uepg.br/oai/requestbicen@uepg.br||mv_fidelis@yahoo.com.bropendoar:2017-07-24T19:38:11Biblioteca Digital de Teses e Dissertações da UEPG - Universidade Estadual de Ponta Grossa (UEPG)false
dc.title.por.fl_str_mv ESTUDO QUÍMICO E BIOATIVIDADES DE Baccharis uncinella DC.
title ESTUDO QUÍMICO E BIOATIVIDADES DE Baccharis uncinella DC.
spellingShingle ESTUDO QUÍMICO E BIOATIVIDADES DE Baccharis uncinella DC.
Ascari, Jociani
Baccharis uncinella
composição química
atividade antimicrobiana
óleo essencial
extratos orgânicos
bioatividade
hemólise
antioxidante
Baccharis uncinella
chemical composition
antimicrobial activity
essential oil
solvent extracts
bioactivity
hemolysis antioxidant
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
title_short ESTUDO QUÍMICO E BIOATIVIDADES DE Baccharis uncinella DC.
title_full ESTUDO QUÍMICO E BIOATIVIDADES DE Baccharis uncinella DC.
title_fullStr ESTUDO QUÍMICO E BIOATIVIDADES DE Baccharis uncinella DC.
title_full_unstemmed ESTUDO QUÍMICO E BIOATIVIDADES DE Baccharis uncinella DC.
title_sort ESTUDO QUÍMICO E BIOATIVIDADES DE Baccharis uncinella DC.
author Ascari, Jociani
author_facet Ascari, Jociani
author_role author
dc.contributor.advisor1.fl_str_mv Nunes, Domingos Sávio
dc.contributor.advisor1ID.fl_str_mv CPF:24650560934
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4781201Y6
dc.contributor.advisor-co1.fl_str_mv Marques, Mariza Boscacci
dc.contributor.advisor-co1ID.fl_str_mv CPF:26387247004
dc.contributor.advisor-co1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4727372D5
dc.contributor.referee1.fl_str_mv Cechinel Filho, Valdir
dc.contributor.referee1ID.fl_str_mv CPF:44347600925
dc.contributor.referee1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4784252A2
dc.contributor.referee2.fl_str_mv Arrúa, Maria Elena Payret
dc.contributor.referee2ID.fl_str_mv CPF:49793144068
dc.contributor.referee2Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4780152D1
dc.contributor.authorID.fl_str_mv CPF:04006705930
dc.contributor.authorLattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4137662H6
dc.contributor.author.fl_str_mv Ascari, Jociani
contributor_str_mv Nunes, Domingos Sávio
Marques, Mariza Boscacci
Cechinel Filho, Valdir
Arrúa, Maria Elena Payret
dc.subject.por.fl_str_mv Baccharis uncinella
composição química
atividade antimicrobiana
óleo essencial
extratos orgânicos
bioatividade
hemólise
antioxidante
topic Baccharis uncinella
composição química
atividade antimicrobiana
óleo essencial
extratos orgânicos
bioatividade
hemólise
antioxidante
Baccharis uncinella
chemical composition
antimicrobial activity
essential oil
solvent extracts
bioactivity
hemolysis antioxidant
CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
dc.subject.eng.fl_str_mv Baccharis uncinella
chemical composition
antimicrobial activity
essential oil
solvent extracts
bioactivity
hemolysis antioxidant
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA
description Baccharis uncinella (Asteraceae), popularly known as vassoura, is little known from the chemical point of view, with only two earlier scientific works on their secondary metabolites - the volatile constituents. In the present study, the essential oil from the leaves of B. uncinella and nine extracts obtained in a Soxhlet apparatus with organic solvents starting from the leaves, barks and log were analyzed by CCD, UV and IV and submitted to activity tests against microorganisms; besides, the interactions between the aqueous extract of the leaves with human erytrocytary cells of the sanguine types O+, O-, A+ and B+ were also analyzed. In the antimicrobial tests, the microfungus Candida albicans, as well as the Gram-positive Bacillus cereus, Enterococcus faecalis, Staphylococcus aureus and S. saprophyticus and the Gram-negative bacteria Escherichia coli, Klebsiella pneumoniae and Pseudomonas aeruginosa were used. The essential oil showed action against B. cereus, S. aureus and C. albicans, in the same way as all of the organic extracts, but the growth of S. saprophyticus was not inhibited by no one of the extracts. The extracts of the barks, when compared with those obtained from other parts of the plant, were active against the largest number of microorganisms and presented the more strong activities. The minimum inhibitory concentration of the essential oil was estimated as 100/mL against S. aureus as well as against C. albicans, wich could be considered a moderate antimicrobial activity. We have isolated small amounts of the three major phenolic components found in the leaves, and the analysis of their UV- and IR-spectra showed the structural features of hydroxylaryl-propenoic acids for these compounds that were found for the first time in this plant species. The analyses of GC-MS of the essential oil produced by hydrodistillation of the leaves collected at the Paraná State presented low monoterpene content (2.29%) and the following sesquiterpenes as the main components: spathulenol 17.20%, caryophyllene oxide 16.20%, transcaryophyllene 10.48%, eudesmol 7.55%, germacrene D 6.28% and cadinol 5.18%. Another oil sample was obtained using the same conditions, but starting with leaves collected in the highland area of Santa Catarina, also presented spathulenol as the main component (15.83%), but it contained 36% in monoterpenes, a very similar composition to those published for samples from the State of Rio Grande do Sul. The interactions among the aqueous extract of the leaves with human erytrocytes isolated from the sanguine types O+, O-, A+ and B+, being verified that the extract presents a non-lytic activity in the analyzed concentrations of 22, 110 and 220/mL. This non-hemolytic profile was observed as similar for all tested blood types. The serological types A+ and O- presented a similar pattern of free Hb oxidation in solution, and the same profile was observed in the new suspension of the precipitate. In the analysis of MEV we observed the qualitative aspects regarding the interaction of the aqueous extract with the architecture of the erythrocyte. In the assays of DPPH with low concentrations, 1.5 x 10-4 and 7.8 x 10-5 mg/mL, it was possible to quantify a partial reduction in the free radicals concentration of 20.10% and 9.25% respectively.
publishDate 2007
dc.date.available.fl_str_mv 2007-07-30
2017-07-24T19:38:11Z
dc.date.issued.fl_str_mv 2007-02-28
dc.date.accessioned.fl_str_mv 2017-07-24T19:38:11Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/masterThesis
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dc.identifier.citation.fl_str_mv ASCARI, Jociani. ESTUDO QUÍMICO E BIOATIVIDADES DE Baccharis uncinella DC.. 2007. 78 f. Dissertação (Mestrado em Química) - UNIVERSIDADE ESTADUAL DE PONTA GROSSA, Ponta Grossa, 2007.
dc.identifier.uri.fl_str_mv http://tede2.uepg.br/jspui/handle/prefix/2120
identifier_str_mv ASCARI, Jociani. ESTUDO QUÍMICO E BIOATIVIDADES DE Baccharis uncinella DC.. 2007. 78 f. Dissertação (Mestrado em Química) - UNIVERSIDADE ESTADUAL DE PONTA GROSSA, Ponta Grossa, 2007.
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dc.publisher.none.fl_str_mv UNIVERSIDADE ESTADUAL DE PONTA GROSSA
dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Química Aplicada
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dc.publisher.department.fl_str_mv Química
publisher.none.fl_str_mv UNIVERSIDADE ESTADUAL DE PONTA GROSSA
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