Ação antibacteriana de diferentes medicações intracanais frente a Enterococcus faecalis

Detalhes bibliográficos
Autor(a) principal: Silva, Ana Rita Marques da
Data de Publicação: 2015
Tipo de documento: Tese
Idioma: por
Título da fonte: Biblioteca Digital de Teses e Dissertações da UEPG
Texto Completo: http://tede2.uepg.br/jspui/handle/prefix/1702
Resumo: The present study aimed to introduce new intracanal medications and evaluate their antimicrobial effect against endodontic microorganisms involved in most of the cases of endodontic failure. The composition of the new formulations included 3% doxycycline hydrochloride (DX) or 3% nitrofurantoin (NIT), and a 2% chlorhexidine gel (CHX) was used as a positive control.This study was divided into three phases. The first phase evaluated the antimicrobial effect of the formulations DX and NIT against Gram-positive bacteria, Enterococcus faecalis and Staphylococcus aureus, Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa, and fungus Candida albicans. The formulations were tested using the traditional agar diffusion test and the determination of the minimum inhibitory concentration (MIC) test. Based on the descriptive analysis of the agar diffusion test, DX presented a better antibacterial effect for most bacteria in the study. NIT values were lower than the CHX’s for all bacteria, with the exception of E. coli. DX and NIT were found ineffective for C. albicans. The descriptive analysis of MIC showed that DX was more effective than NIT, but when compared with CHX, it presented a lower antimicrobial effect. The second phase evaluated the antimicrobial effect against E. faecalis on the external root surface and the ability of diffusion through dentinal tubules of the new intracanal medications. Fifty human canine teeth were instrumented, sterilized and randomly divided into 5 groups of 10 teeth each, according to the formulation used as intracanal dressing: teeth sealed without medication (SM), negative control; CHX, positive control; placebo gel (PC); NIT and DX. After filling the root canals with the formulations, the teeth were incubated in the agar plates seeded with E. faecalis. The analysis of the halos revealed that DX had a larger inhibitory zone than CHX, while PC and NIT showed no antibacterial effect on the external root surface. The last phase evaluated the antimicrobial activity on infected root canals with E.faecalis. The chosen formulation was DX, as it obtained the best results on previous phases. Microbial samples were collected during three different time periods. The first sample (S1) was collected 21 days after contamination with E. faecalis; the second sample (S2) was collected 14 days under the effect of the intracanal medication, and the third sample (S3) 7 days after replacing the medication by BHI (Brain Heart Infusion) broth. The count of Colony Forming Units (CFU/mL) was statistically analyzed by repeated measures test (ANOVA) (p<0,05). All intracanal dressings significantly reduced the number of bacterial cells in the root canal after 14 days with medication (S2). After a period of 7 days with BHI broth (S3), the CFU counts of E. faecalis remained at low values in DX and CHX groups. The SM group, however, showed a significant increase of CFU in this period, to values similar to those of the initial contamination. These results show that DX was effective in eliminating E. faecalis from the root canal system, significantly decreasing the number of microorganisms after 14 days with medication and suggesting some substantivity after 7 days.Conclusion: From the two formulations suggested in this study, DX presented better antimicrobial effect against the microorganisms in the study (agar diffusion and MIC),supported by the effect on the external root surface and inside the root canal system. This new formulation containing DX can be considered as a possible intracanal dressing, namely in cases of endodontic failure.
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spelling Gomes, João CarlosCPF:33867771987http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4793213H4Carvalho, Manuel FontesPinto, Miguel Fernando Silva GonçalvesFerreira, Manuel MarquesSilva, Mário Jorge Rebolho Fernandes daCapelas, José Antonio Macedo CarvalhoSantos, Elizabete Brasil dosCPF:77977670763http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4792032H6CPF:00000000000001http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4330136T2Silva, Ana Rita Marques da2017-07-24T19:21:58Z2016-04-262017-07-24T19:21:58Z2015-07-30SILVA, Ana Rita Marques da. Ação antibacteriana de diferentes medicações intracanais frente a Enterococcus faecalis. 2015. 173 f. Tese (Doutorado em Clinica Integrada, Dentística Restauradora e Periodontia) - UNIVERSIDADE ESTADUAL DE PONTA GROSSA, Porto, 2015.http://tede2.uepg.br/jspui/handle/prefix/1702The present study aimed to introduce new intracanal medications and evaluate their antimicrobial effect against endodontic microorganisms involved in most of the cases of endodontic failure. The composition of the new formulations included 3% doxycycline hydrochloride (DX) or 3% nitrofurantoin (NIT), and a 2% chlorhexidine gel (CHX) was used as a positive control.This study was divided into three phases. The first phase evaluated the antimicrobial effect of the formulations DX and NIT against Gram-positive bacteria, Enterococcus faecalis and Staphylococcus aureus, Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa, and fungus Candida albicans. The formulations were tested using the traditional agar diffusion test and the determination of the minimum inhibitory concentration (MIC) test. Based on the descriptive analysis of the agar diffusion test, DX presented a better antibacterial effect for most bacteria in the study. NIT values were lower than the CHX’s for all bacteria, with the exception of E. coli. DX and NIT were found ineffective for C. albicans. The descriptive analysis of MIC showed that DX was more effective than NIT, but when compared with CHX, it presented a lower antimicrobial effect. The second phase evaluated the antimicrobial effect against E. faecalis on the external root surface and the ability of diffusion through dentinal tubules of the new intracanal medications. Fifty human canine teeth were instrumented, sterilized and randomly divided into 5 groups of 10 teeth each, according to the formulation used as intracanal dressing: teeth sealed without medication (SM), negative control; CHX, positive control; placebo gel (PC); NIT and DX. After filling the root canals with the formulations, the teeth were incubated in the agar plates seeded with E. faecalis. The analysis of the halos revealed that DX had a larger inhibitory zone than CHX, while PC and NIT showed no antibacterial effect on the external root surface. The last phase evaluated the antimicrobial activity on infected root canals with E.faecalis. The chosen formulation was DX, as it obtained the best results on previous phases. Microbial samples were collected during three different time periods. The first sample (S1) was collected 21 days after contamination with E. faecalis; the second sample (S2) was collected 14 days under the effect of the intracanal medication, and the third sample (S3) 7 days after replacing the medication by BHI (Brain Heart Infusion) broth. The count of Colony Forming Units (CFU/mL) was statistically analyzed by repeated measures test (ANOVA) (p<0,05). All intracanal dressings significantly reduced the number of bacterial cells in the root canal after 14 days with medication (S2). After a period of 7 days with BHI broth (S3), the CFU counts of E. faecalis remained at low values in DX and CHX groups. The SM group, however, showed a significant increase of CFU in this period, to values similar to those of the initial contamination. These results show that DX was effective in eliminating E. faecalis from the root canal system, significantly decreasing the number of microorganisms after 14 days with medication and suggesting some substantivity after 7 days.Conclusion: From the two formulations suggested in this study, DX presented better antimicrobial effect against the microorganisms in the study (agar diffusion and MIC),supported by the effect on the external root surface and inside the root canal system. This new formulation containing DX can be considered as a possible intracanal dressing, namely in cases of endodontic failure.Este estudo teve como objetivo investigar novas formulações de medicações intracanal e verificar a ação antimicrobiana destas em microrganismos frequentemente implicados em casos de insucesso endodôntico. As formulações estudadas continham doxiciclina a 3% (DX) ou nitrofurantoína a 3% (NIT), tendo sido usado como controlo positivo um gel de clorohexidina a 2% (CHX).O estudo foi dividido em três fases experimentais. Na primeira fase, foi analisado o efeito antimicrobiano das formulações DX e NIT, na presença das bactérias de Gram positivo, Enterococcus faecalis e Staphylococcus aureus, das bactérias de Gram negativo, Escherichia coli e Pseudomonas aeruginosa, e do fungo Candida albicans através de testes de difusão em agar e da determinação da concentração inibitória mínima (CIM). Com base na análise descritiva dos resultados da difusão em agar, a DX foi a que apresentou maior efeito antimicrobiano, na generalidade das bactérias estudadas. Os resultados da NITforam inferiores aos da CHX em todas as bactérias do estudo, à exceção de E. coli.Tanto a DX como a NIT evidenciaram-se inativas para C. albicans. A análise descritiva da determinação da CIM revelou que a DX apresentou maior eficácia comparativamente com a NIT, mas resultados inferiores quando comparada com a CHX.Na segunda fase, as novas formulações foram avaliadas pela sua ação antimicrobiana, na superfície externa radicular, contra E. faecalis e eventual capacidade de difusão através dos canalículos dentinários. Foram usados 50 dentes humanos (caninos), previamente instrumentados e esterilizados, distribuídos aleatoriamente em cinco grupos (n=10), e atribuída uma formulação diferente a cada grupo: sem medicação (SM), controlo negativo; gel de clorohexidina a 2% (CHX), controlo positivo; gel placebo (PC); gel de nitrofurantoína a 3% (NIT); e gel de doxiciclina a 3% (DX). Preencheu-se o interior dos canais com as respetivas formulações e fez-se a incubação dos dentes em placas de agar com E. faecalis. A análise dos halos dos grupos em estudo demonstrou que a DX tem maior atividade na face externa da raiz do que a CHX, e que a NIT e o PC não evidenciaram ação antibacteriana no exterior da raiz. Na terceira e última fase, avaliou-se a ação antimicrobiana, em canais radiculares contaminados com E. faecalis. A formulação usada nesta fase foi a DX, uma vez que foi a que obteve melhores resultados nas duas fases anteriores. Efetuaram-se colheitas bacterianas em três períodos de tempo distintos. A primeira colheita (S1) realizou-se 21 dias depois da contaminação com E. faecalis; a segunda colheita (S2) foi efetuada 14 dias após a aplicação das formulações e a terceira colheita (S3), 7 dias depois da substituição das formulações por caldo de BHI (“Brain Heart Infusion”). A contagem das Unidades Formadoras de Colónias (UFC/mL) foi analisada através do teste de medidas repetidas (ANOVA) (p<0,05). Todas as novas formulações diminuíram significativamente o número de células bacterianas no interior dos canais após os 14 dias (S2) sob a ação da medicação intracanal. Depois do período de 7 dias com o caldo de BHI (S3), os valores de UFC de E. faecalis mantiveram-se em valores baixos nos grupos DX e CHX. No grupo SM verificou-se um aumento significativo para valores similares aos da contaminação inicial.Verificou-se, assim, que a DX foi eficaz na desinfeção dos canais, reduzindo significativamente o número de microrganismos após 14 dias e demonstrando alguma substantividade após 7 dias.Conclusão: Das duas formulações sugeridas neste estudo, o gel DX apresentou uma eficácia maior sobre os microrganismos estudados (difusão em agar e CIM), corroborado pela ação na superfície externa da raiz e no interior dos canais radiculares. Na formulação apresentada, o gel DX pode ser considerado como uma medicação intracanal alternativa, nomeadamente nos casos de insucesso endodôntico.Made available in DSpace on 2017-07-24T19:21:58Z (GMT). No. of bitstreams: 1 Ana Rita.pdf: 19490568 bytes, checksum: 013237cc37f3177397fb7ac6f745899e (MD5) Previous issue date: 2015-07-30application/pdfporUNIVERSIDADE ESTADUAL DE PONTA GROSSAPrograma de Pós-Graduação em OdontologiaUEPGBRClinica Integrada, Dentística Restauradora e PeriodontiaEnterococcus faecalisendodontiaDoxiciclinaNitrofurantoínaClorohexidinaEnterococcus faecalisendodonticsDoxycyclineNitrofurantoinChlorhexidineCNPQ::CIENCIAS DA SAUDE::ODONTOLOGIAAção antibacteriana de diferentes medicações intracanais frente a Enterococcus faecalisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UEPGinstname:Universidade Estadual de Ponta Grossa (UEPG)instacron:UEPGORIGINALAna Rita.pdfapplication/pdf19490568http://tede2.uepg.br/jspui/bitstream/prefix/1702/1/Ana%20Rita.pdf013237cc37f3177397fb7ac6f745899eMD51prefix/17022017-07-24 16:21:58.573oai:tede2.uepg.br:prefix/1702Biblioteca Digital de Teses e Dissertaçõeshttps://tede2.uepg.br/jspui/PUBhttp://tede2.uepg.br/oai/requestbicen@uepg.br||mv_fidelis@yahoo.com.bropendoar:2017-07-24T19:21:58Biblioteca Digital de Teses e Dissertações da UEPG - Universidade Estadual de Ponta Grossa (UEPG)false
dc.title.por.fl_str_mv Ação antibacteriana de diferentes medicações intracanais frente a Enterococcus faecalis
title Ação antibacteriana de diferentes medicações intracanais frente a Enterococcus faecalis
spellingShingle Ação antibacteriana de diferentes medicações intracanais frente a Enterococcus faecalis
Silva, Ana Rita Marques da
Enterococcus faecalis
endodontia
Doxiciclina
Nitrofurantoína
Clorohexidina
Enterococcus faecalis
endodontics
Doxycycline
Nitrofurantoin
Chlorhexidine
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
title_short Ação antibacteriana de diferentes medicações intracanais frente a Enterococcus faecalis
title_full Ação antibacteriana de diferentes medicações intracanais frente a Enterococcus faecalis
title_fullStr Ação antibacteriana de diferentes medicações intracanais frente a Enterococcus faecalis
title_full_unstemmed Ação antibacteriana de diferentes medicações intracanais frente a Enterococcus faecalis
title_sort Ação antibacteriana de diferentes medicações intracanais frente a Enterococcus faecalis
author Silva, Ana Rita Marques da
author_facet Silva, Ana Rita Marques da
author_role author
dc.contributor.advisor1.fl_str_mv Gomes, João Carlos
dc.contributor.advisor1ID.fl_str_mv CPF:33867771987
dc.contributor.advisor1Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4793213H4
dc.contributor.advisor-co1.fl_str_mv Carvalho, Manuel Fontes
dc.contributor.referee1.fl_str_mv Pinto, Miguel Fernando Silva Gonçalves
dc.contributor.referee2.fl_str_mv Ferreira, Manuel Marques
dc.contributor.referee3.fl_str_mv Silva, Mário Jorge Rebolho Fernandes da
dc.contributor.referee4.fl_str_mv Capelas, José Antonio Macedo Carvalho
dc.contributor.referee5.fl_str_mv Santos, Elizabete Brasil dos
dc.contributor.referee5ID.fl_str_mv CPF:77977670763
dc.contributor.referee5Lattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4792032H6
dc.contributor.authorID.fl_str_mv CPF:00000000000001
dc.contributor.authorLattes.fl_str_mv http://buscatextual.cnpq.br/buscatextual/visualizacv.do?id=K4330136T2
dc.contributor.author.fl_str_mv Silva, Ana Rita Marques da
contributor_str_mv Gomes, João Carlos
Carvalho, Manuel Fontes
Pinto, Miguel Fernando Silva Gonçalves
Ferreira, Manuel Marques
Silva, Mário Jorge Rebolho Fernandes da
Capelas, José Antonio Macedo Carvalho
Santos, Elizabete Brasil dos
dc.subject.por.fl_str_mv Enterococcus faecalis
endodontia
Doxiciclina
Nitrofurantoína
Clorohexidina
topic Enterococcus faecalis
endodontia
Doxiciclina
Nitrofurantoína
Clorohexidina
Enterococcus faecalis
endodontics
Doxycycline
Nitrofurantoin
Chlorhexidine
CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
dc.subject.eng.fl_str_mv Enterococcus faecalis
endodontics
Doxycycline
Nitrofurantoin
Chlorhexidine
dc.subject.cnpq.fl_str_mv CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA
description The present study aimed to introduce new intracanal medications and evaluate their antimicrobial effect against endodontic microorganisms involved in most of the cases of endodontic failure. The composition of the new formulations included 3% doxycycline hydrochloride (DX) or 3% nitrofurantoin (NIT), and a 2% chlorhexidine gel (CHX) was used as a positive control.This study was divided into three phases. The first phase evaluated the antimicrobial effect of the formulations DX and NIT against Gram-positive bacteria, Enterococcus faecalis and Staphylococcus aureus, Gram-negative bacteria, Escherichia coli and Pseudomonas aeruginosa, and fungus Candida albicans. The formulations were tested using the traditional agar diffusion test and the determination of the minimum inhibitory concentration (MIC) test. Based on the descriptive analysis of the agar diffusion test, DX presented a better antibacterial effect for most bacteria in the study. NIT values were lower than the CHX’s for all bacteria, with the exception of E. coli. DX and NIT were found ineffective for C. albicans. The descriptive analysis of MIC showed that DX was more effective than NIT, but when compared with CHX, it presented a lower antimicrobial effect. The second phase evaluated the antimicrobial effect against E. faecalis on the external root surface and the ability of diffusion through dentinal tubules of the new intracanal medications. Fifty human canine teeth were instrumented, sterilized and randomly divided into 5 groups of 10 teeth each, according to the formulation used as intracanal dressing: teeth sealed without medication (SM), negative control; CHX, positive control; placebo gel (PC); NIT and DX. After filling the root canals with the formulations, the teeth were incubated in the agar plates seeded with E. faecalis. The analysis of the halos revealed that DX had a larger inhibitory zone than CHX, while PC and NIT showed no antibacterial effect on the external root surface. The last phase evaluated the antimicrobial activity on infected root canals with E.faecalis. The chosen formulation was DX, as it obtained the best results on previous phases. Microbial samples were collected during three different time periods. The first sample (S1) was collected 21 days after contamination with E. faecalis; the second sample (S2) was collected 14 days under the effect of the intracanal medication, and the third sample (S3) 7 days after replacing the medication by BHI (Brain Heart Infusion) broth. The count of Colony Forming Units (CFU/mL) was statistically analyzed by repeated measures test (ANOVA) (p<0,05). All intracanal dressings significantly reduced the number of bacterial cells in the root canal after 14 days with medication (S2). After a period of 7 days with BHI broth (S3), the CFU counts of E. faecalis remained at low values in DX and CHX groups. The SM group, however, showed a significant increase of CFU in this period, to values similar to those of the initial contamination. These results show that DX was effective in eliminating E. faecalis from the root canal system, significantly decreasing the number of microorganisms after 14 days with medication and suggesting some substantivity after 7 days.Conclusion: From the two formulations suggested in this study, DX presented better antimicrobial effect against the microorganisms in the study (agar diffusion and MIC),supported by the effect on the external root surface and inside the root canal system. This new formulation containing DX can be considered as a possible intracanal dressing, namely in cases of endodontic failure.
publishDate 2015
dc.date.issued.fl_str_mv 2015-07-30
dc.date.available.fl_str_mv 2016-04-26
2017-07-24T19:21:58Z
dc.date.accessioned.fl_str_mv 2017-07-24T19:21:58Z
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dc.type.driver.fl_str_mv info:eu-repo/semantics/doctoralThesis
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dc.identifier.citation.fl_str_mv SILVA, Ana Rita Marques da. Ação antibacteriana de diferentes medicações intracanais frente a Enterococcus faecalis. 2015. 173 f. Tese (Doutorado em Clinica Integrada, Dentística Restauradora e Periodontia) - UNIVERSIDADE ESTADUAL DE PONTA GROSSA, Porto, 2015.
dc.identifier.uri.fl_str_mv http://tede2.uepg.br/jspui/handle/prefix/1702
identifier_str_mv SILVA, Ana Rita Marques da. Ação antibacteriana de diferentes medicações intracanais frente a Enterococcus faecalis. 2015. 173 f. Tese (Doutorado em Clinica Integrada, Dentística Restauradora e Periodontia) - UNIVERSIDADE ESTADUAL DE PONTA GROSSA, Porto, 2015.
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dc.publisher.program.fl_str_mv Programa de Pós-Graduação em Odontologia
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dc.publisher.department.fl_str_mv Clinica Integrada, Dentística Restauradora e Periodontia
publisher.none.fl_str_mv UNIVERSIDADE ESTADUAL DE PONTA GROSSA
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