Análise parasitológica e morfológica dos efeitos do artesunato e sinvastatina em camundongos esquistossomóticos e dislipidêmicos

• Autor(a) principal: Alencar, Alba Cristina Miranda de Barros
• Data de Publicação: 2015
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/14373
• topic: Schistosoma mansoni; Murine model; Hight fat diet; Simvastatin; Artesunate; Parasitological analysis; Adult worms; Laser confocal microscopy; Scanning microscopy; Schistosoma mansoni; Modelo murino; Dieta hiperlipídica; Sinvastatina; Artesunato; Análise parasitológica; Vermes adultos; Microscopia laser confocal; Microscopia de varredura; Esquistossomose; Dieta hiperlipídica; Sinvastatina; Análise parasitológica; Vermes intestinais e parasitários; Microscopia eletrônica de varredura; CNPQ::CIENCIAS BIOLOGICAS::PARASITOLOGIA::HELMINTOLOGIA DE PARASITOS

The long-term administration of a high fat diet favors the development of adult worms and Schistosoma mansoni infection in mice. Statins are hypolipidemic drugs that cause morphological changes, mainly in the reproductive system of S. mansoni adult. The artesunate, an antimalarial drug, reduces the survival time of the worms and the elimination of eggs in vitro. It is unknow the effectiveness of these drugs in schistosomiasis hyperlipidemic mice. Female mice, Swiss Webster received a high-fat diet (29% fat) or standard diet (12% lipids). After six months were infected subcutaneously with 80 cercariae of S. mansoni (BH strain). After 9 weeks of infection, the animals were treated with simvastatin or artesunate, which resulted in the experimental groups: standard diet or a high fat diet untreated standard diet treated with simvastatin or five doses standard diet treated with artesunate or five doses high-fat diet treated with simvastatin with or five doses high-fat diet and treated with artesunate with one or five doses. Two weeks after treatment, the mice were sacrificed to collect blood (cholesterol levels), intestine (oogram) and worms (determination of worm burden reduction, morphometric analysis, morphological, confocal microscopy and scanning). Serum biochemical analysis showed that the animals that received the high-fat diet exhibited higher total cholesterol levels than controls. Treatment with simvastatin had hypolipidemic action (regardless of diet), reduced parasitic load, and caused changes in the reproductive system and especially the integument of worms recovered from dyslipidemic mice. The difference between the simvastatin doses did not appear to interfere with the observed changes. The artesunate promoved intense reduction in mean worm recovery and increase in parasitic rate of reduction in dyslipidemic group. The reproductive system and the integument showed moderate to intense change, especially when related to diet. The effect of artesunate was higher when given five doses of worms recovered dyslipidemic mice. It is concluded that the drugs used in this study had better in groups of dyslipidemic mice.