Análise microbiológica das mãos de cirurgiões em um hospital público de São Luís - MA, Brasil
Autor(a) principal: | |
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Data de Publicação: | 2023 |
Outros Autores: | |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UERJ |
Texto Completo: | http://www.bdtd.uerj.br/handle/1/20572 |
Resumo: | Antisepsis of the hands of healthcare professionals working in an operating room is one of the most important steps in the healthcare process, since it can cause the cross-transfer of potentially pathogenic microorganisms to surgical sites, and is one of the most important goals in the patient safety program. This cross-sectional epidemiological study aimed to analyze the prevalence of microorganisms on the hands of surgeons at a public university hospital before and after surgical antisepsis, as well as to assess the level of knowledge of surgeons about patient safety goals. A swab was taken from the dominant hand of each doctor, before and after the hand-washing process. The samples were placed in Stuart transport medium and delivered to a private clinical analysis laboratory in São Luís-Maranhão. The microorganisms were identified by mass spectrophotometry (MALDI-TOF), and antimicrobial susceptibility testing (ASCT) was carried out. The results showed a high frequency (100%) of microorganisms before hand antisepsis, decreasing significantly (p<0.05) to 27.5% after antisepsis. Gram-positive cocci and rods were the most commonly identified germs after hand washing, followed by gram-negative bacilli species. The effectiveness of hand antisepsis was calculated at 72.5%. At the same time, a validated questionnaire revealed that only 13.6% of surgeons were fully aware of the six international patient safety goals, with the fourth goal (ensuring surgery at the correct site, procedure and patient) being the most widely known by 66.7% of those assessed. It was concluded that even after antisepsis, microorganisms of various potentially pathogenic species can remain on surgeons' hands, which points to a program of permanent education and continuous surveillance in order to generate a process of quality improvements in care |
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Nunes, Rodolfo Acatauassúhttp://lattes.cnpq.br/5246655943457913Souza, Rosângela Cipriano dehttp://lattes.cnpq.br/0760406534248737Pitombo, Marcos Bettinihttp://lattes.cnpq.br/0195916564426040Marques, Elizabeth de Andradehttp://lattes.cnpq.br/5959485578597640Torres, Orlando Jorge Martinshttp://lattes.cnpq.br/6448439573128558Lages, Joyce Santoshttp://lattes.cnpq.br/3197054961853447http://lattes.cnpq.br/1373036741608175Serra Neto, Arturartur.neto@huufma.br2023-11-06T20:39:50Z2023-09-13SERRA NETO, Artur. Análise microbiológica das mãos de cirurgiões em um hospital público de São Luís - MA, Brasil. 2023. 91 f. Tese (Doutorado em Ciências Médicas) - Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2023.http://www.bdtd.uerj.br/handle/1/20572Antisepsis of the hands of healthcare professionals working in an operating room is one of the most important steps in the healthcare process, since it can cause the cross-transfer of potentially pathogenic microorganisms to surgical sites, and is one of the most important goals in the patient safety program. This cross-sectional epidemiological study aimed to analyze the prevalence of microorganisms on the hands of surgeons at a public university hospital before and after surgical antisepsis, as well as to assess the level of knowledge of surgeons about patient safety goals. A swab was taken from the dominant hand of each doctor, before and after the hand-washing process. The samples were placed in Stuart transport medium and delivered to a private clinical analysis laboratory in São Luís-Maranhão. The microorganisms were identified by mass spectrophotometry (MALDI-TOF), and antimicrobial susceptibility testing (ASCT) was carried out. The results showed a high frequency (100%) of microorganisms before hand antisepsis, decreasing significantly (p<0.05) to 27.5% after antisepsis. Gram-positive cocci and rods were the most commonly identified germs after hand washing, followed by gram-negative bacilli species. The effectiveness of hand antisepsis was calculated at 72.5%. At the same time, a validated questionnaire revealed that only 13.6% of surgeons were fully aware of the six international patient safety goals, with the fourth goal (ensuring surgery at the correct site, procedure and patient) being the most widely known by 66.7% of those assessed. It was concluded that even after antisepsis, microorganisms of various potentially pathogenic species can remain on surgeons' hands, which points to a program of permanent education and continuous surveillance in order to generate a process of quality improvements in careA antissepsia das mãos dos profissionais de saúde que atuam em um centro cirúrgico é um dos passos mais importantes no processo de assistência à saúde, uma vez que pode provocar a transferência cruzada de microrganismos potencialmente patogênicos aos sítios operatórios, sendo uma das metas mais importantes no programa de segurança do paciente. Este estudo epidemiológico transversal teve como objetivo analisar a prevalência de microrganismos nas mãos de cirurgiões de um hospital público universitário antes e depois da antissepsia cirúrgica, além de avaliar o grau de conhecimento dos cirurgiões sobre as metas de segurança do paciente. Foi colhido swab proveniente da mão dominante de cada médico, antes e depois do processo de antissepsia cirúrgica. As amostras foram colocadas em meio de transporte Stuart e entregues a um Laboratório de Análises Clínicas privado de São Luís-Maranhão. Os microrganismos foram identificados por espectrofotometria de massa (MALDI-TOF), e os testes de sensibilidade aos antimicrobianos (TSA) foram realizados. Os resultados mostraram uma frequência elevada (100%) de microrganismos antes da antissepsia das mãos, diminuindo significativamente (p<0,05) para 27,5%, após a antissepsia. Os Cocos e os Bastonetes gram-positivos foram os germes mais comumente identificados após a lavagem das mãos, seguidos das espécies de bacilos gram-negativos. A eficácia da antissepsia das mãos foi calculada em 72,5%. Paralelamente, em questionário validado, verificou-se que apenas 13,6% dos cirurgiões conheciam totalmente as seis metas internacionais de segurança do paciente, sendo a mais conhecida, por 66,7% dos avaliados, a quarta meta (assegurar cirurgia em local de intervenção, procedimento e paciente corretos). Concluiu-se que mesmo após a antissepsia podem permanecer microorganismos de várias espécies potencialmente patogênicas nas mãos dos cirurgiões, o que aponta para um programa de educação permanente e vigilância contínua a fim de gerar um processo de melhorias de qualidade na assistência.Submitted by Felipe CB/A (felipebibliotecario@gmail.com) on 2023-11-06T20:39:50Z No. of bitstreams: 1 Tese - Artur Serra Neto - 2023 - Completa.pdf: 2402592 bytes, checksum: 0e46140ef7ddc22a193d5d90f3ff7bbd (MD5)Made available in DSpace on 2023-11-06T20:39:50Z (GMT). No. of bitstreams: 1 Tese - Artur Serra Neto - 2023 - Completa.pdf: 2402592 bytes, checksum: 0e46140ef7ddc22a193d5d90f3ff7bbd (MD5) Previous issue date: 2023-09-13application/pdfporUniversidade do Estado do Rio de JaneiroPrograma de Pós-Graduação em Ciências MédicasUERJBrasilCentro Biomédico::Faculdade de Ciências MédicasHandsAntisepsisSurgeonsSurgical wound infectionCross infectionMãosAntissepsiaCirurgiões – AntissepsiaInfecção sítio cirúrgicoInfecção hospitalarCentro cirúrgico hospitalar – MaranhãoSegurança do paciente – NormasCIENCIAS DA SAUDE::MEDICINAAnálise microbiológica das mãos de cirurgiões em um hospital público de São Luís - MA, BrasilMicrobiological analysis of surgeons' hands in a public hospital in São Luis, Maranhão State, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UERJinstname:Universidade do Estado do Rio de Janeiro (UERJ)instacron:UERJORIGINALTese - Artur Serra Neto - 2023 - Completa.pdfTese - Artur Serra Neto - 2023 - Completa.pdfapplication/pdf2402592http://www.bdtd.uerj.br/bitstream/1/20572/2/Tese+-+Artur+Serra+Neto+-+2023+-+Completa.pdf0e46140ef7ddc22a193d5d90f3ff7bbdMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82123http://www.bdtd.uerj.br/bitstream/1/20572/1/license.txte5502652da718045d7fcd832b79fca29MD511/205722024-02-26 15:59:54.292oai:www.bdtd.uerj.br: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Biblioteca Digital de Teses e Dissertaçõeshttp://www.bdtd.uerj.br/PUBhttps://www.bdtd.uerj.br:8443/oai/requestbdtd.suporte@uerj.bropendoar:29032024-02-26T18:59:54Biblioteca Digital de Teses e Dissertações da UERJ - Universidade do Estado do Rio de Janeiro (UERJ)false |
dc.title.por.fl_str_mv |
Análise microbiológica das mãos de cirurgiões em um hospital público de São Luís - MA, Brasil |
dc.title.alternative.eng.fl_str_mv |
Microbiological analysis of surgeons' hands in a public hospital in São Luis, Maranhão State, Brazil |
title |
Análise microbiológica das mãos de cirurgiões em um hospital público de São Luís - MA, Brasil |
spellingShingle |
Análise microbiológica das mãos de cirurgiões em um hospital público de São Luís - MA, Brasil Serra Neto, Artur Hands Antisepsis Surgeons Surgical wound infection Cross infection Mãos Antissepsia Cirurgiões – Antissepsia Infecção sítio cirúrgico Infecção hospitalar Centro cirúrgico hospitalar – Maranhão Segurança do paciente – Normas CIENCIAS DA SAUDE::MEDICINA |
title_short |
Análise microbiológica das mãos de cirurgiões em um hospital público de São Luís - MA, Brasil |
title_full |
Análise microbiológica das mãos de cirurgiões em um hospital público de São Luís - MA, Brasil |
title_fullStr |
Análise microbiológica das mãos de cirurgiões em um hospital público de São Luís - MA, Brasil |
title_full_unstemmed |
Análise microbiológica das mãos de cirurgiões em um hospital público de São Luís - MA, Brasil |
title_sort |
Análise microbiológica das mãos de cirurgiões em um hospital público de São Luís - MA, Brasil |
author |
Serra Neto, Artur |
author_facet |
Serra Neto, Artur artur.neto@huufma.br |
author_role |
author |
author2 |
artur.neto@huufma.br |
author2_role |
author |
dc.contributor.advisor1.fl_str_mv |
Nunes, Rodolfo Acatauassú |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5246655943457913 |
dc.contributor.advisor-co1.fl_str_mv |
Souza, Rosângela Cipriano de |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/0760406534248737 |
dc.contributor.referee1.fl_str_mv |
Pitombo, Marcos Bettini |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/0195916564426040 |
dc.contributor.referee2.fl_str_mv |
Marques, Elizabeth de Andrade |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/5959485578597640 |
dc.contributor.referee3.fl_str_mv |
Torres, Orlando Jorge Martins |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/6448439573128558 |
dc.contributor.referee4.fl_str_mv |
Lages, Joyce Santos |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/3197054961853447 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/1373036741608175 |
dc.contributor.author.fl_str_mv |
Serra Neto, Artur artur.neto@huufma.br |
contributor_str_mv |
Nunes, Rodolfo Acatauassú Souza, Rosângela Cipriano de Pitombo, Marcos Bettini Marques, Elizabeth de Andrade Torres, Orlando Jorge Martins Lages, Joyce Santos |
dc.subject.eng.fl_str_mv |
Hands Antisepsis Surgeons Surgical wound infection Cross infection |
topic |
Hands Antisepsis Surgeons Surgical wound infection Cross infection Mãos Antissepsia Cirurgiões – Antissepsia Infecção sítio cirúrgico Infecção hospitalar Centro cirúrgico hospitalar – Maranhão Segurança do paciente – Normas CIENCIAS DA SAUDE::MEDICINA |
dc.subject.por.fl_str_mv |
Mãos Antissepsia Cirurgiões – Antissepsia Infecção sítio cirúrgico Infecção hospitalar Centro cirúrgico hospitalar – Maranhão Segurança do paciente – Normas |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::MEDICINA |
description |
Antisepsis of the hands of healthcare professionals working in an operating room is one of the most important steps in the healthcare process, since it can cause the cross-transfer of potentially pathogenic microorganisms to surgical sites, and is one of the most important goals in the patient safety program. This cross-sectional epidemiological study aimed to analyze the prevalence of microorganisms on the hands of surgeons at a public university hospital before and after surgical antisepsis, as well as to assess the level of knowledge of surgeons about patient safety goals. A swab was taken from the dominant hand of each doctor, before and after the hand-washing process. The samples were placed in Stuart transport medium and delivered to a private clinical analysis laboratory in São Luís-Maranhão. The microorganisms were identified by mass spectrophotometry (MALDI-TOF), and antimicrobial susceptibility testing (ASCT) was carried out. The results showed a high frequency (100%) of microorganisms before hand antisepsis, decreasing significantly (p<0.05) to 27.5% after antisepsis. Gram-positive cocci and rods were the most commonly identified germs after hand washing, followed by gram-negative bacilli species. The effectiveness of hand antisepsis was calculated at 72.5%. At the same time, a validated questionnaire revealed that only 13.6% of surgeons were fully aware of the six international patient safety goals, with the fourth goal (ensuring surgery at the correct site, procedure and patient) being the most widely known by 66.7% of those assessed. It was concluded that even after antisepsis, microorganisms of various potentially pathogenic species can remain on surgeons' hands, which points to a program of permanent education and continuous surveillance in order to generate a process of quality improvements in care |
publishDate |
2023 |
dc.date.accessioned.fl_str_mv |
2023-11-06T20:39:50Z |
dc.date.issued.fl_str_mv |
2023-09-13 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
SERRA NETO, Artur. Análise microbiológica das mãos de cirurgiões em um hospital público de São Luís - MA, Brasil. 2023. 91 f. Tese (Doutorado em Ciências Médicas) - Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2023. |
dc.identifier.uri.fl_str_mv |
http://www.bdtd.uerj.br/handle/1/20572 |
identifier_str_mv |
SERRA NETO, Artur. Análise microbiológica das mãos de cirurgiões em um hospital público de São Luís - MA, Brasil. 2023. 91 f. Tese (Doutorado em Ciências Médicas) - Faculdade de Ciências Médicas, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2023. |
url |
http://www.bdtd.uerj.br/handle/1/20572 |
dc.language.iso.fl_str_mv |
por |
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por |
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Universidade do Estado do Rio de Janeiro |
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UERJ |
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Brasil |
dc.publisher.department.fl_str_mv |
Centro Biomédico::Faculdade de Ciências Médicas |
publisher.none.fl_str_mv |
Universidade do Estado do Rio de Janeiro |
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