Produção de porfirinas por corynebacterium diphtheriae

• Autor(a) principal: Alves, Gabriela Batista
• Data de Publicação: 2009
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/15723
• topic: porphyrin; Corynebacterium diphtheriae; porfirinas, Corynebacterium diphtheriae; CNPQ::CIENCIAS EXATAS E DA TERRA::QUIMICA

Porphyrin production by Corynebacterium diphtheriae is an old research interest but less attention has been given to the subject in the areas of chemistry and medical sciences. The object of this study is to compare the influence of different culture media in the production of porphyrins by Corynebacterium diphtheriae, to determine the iron concentration influence, to compare quantitatively the production of porphyrins by different samples of diphtheria bacilli and to characterize the porphyrins identities. UV-visible absorption and fluorescence spectroscopies were chosen as analytical tools. The culture medium described by Mueller (1939), for the production of toxin, was more efficient than the medium B of King, used in the laboratory diagnosis of diphtheria. The iron concentration in the cultivation of Corynebacterium diphtheriae influence the production of porphyrin. High concentrations inhibit their production. The iron concentration for maximum production of porphyrins is 0.20  g / mL. Among the 11 studied diphtheria bacillus, the sample HC03, fermented sucrose and toxinogenic, isolated from a case of endocarditis, was the largest producer of porphyrin. The sample of Corynebacterium ulcerans ATCC, unfermented sucrose and toxinogenic was the sample that produced less. All 11 samples showed the same profile of the fluorescence spectrum, suggesting that the porphyrin produced is the same in the samples studied. The tests made to characterize the type of porphyrin produced suggests that this is a coproporphyrin. However, the absorption and fluorescence spectra can not determine which isomer(s) is(are) present