Resposta de anticorpos induzida pela vacina conjugada contra Neisseria meningitidis C em crianças e adolescentes infectados pelo HIV

• Autor(a) principal: Castro, Raquel Bernardo Nana de
• Data de Publicação: 2015
• Outros Autores: nutricionista gmail.com
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/18260
• topic: Neisseria meningitidis serogroup C; HIV; Meningococcal serogroup C conjugate vaccine; Bactericidal antibodies; Neisseria meningitidis sorogrupo C; HIV; Vacina meningocócica conjugada sorogrupo C; Anticorpos bactericidas; CIENCIAS BIOLOGICAS::MICROBIOLOGIA::MICROBIOLOGIA APLICADA

Human infections caused by Neisseria meningitidis remains a serious public health problem. A vaccine against N. meningitidis serogroup C (MenC) has been shown to be safe and immunogenic in many high-risk populations with varying results depending on the degree of immunosuppression. However, there are few data on the use of this vaccine in children and adolescents infected with HIV. Therefore, the objective of this study was to evaluate the antibody response of patients infected with HIV after immunization against MenC. A prospective cohort study of 154 children and adolescents 2-18 years of age vertically infected by HIV was performed. All subjects received two doses of conjugated vaccine intramuscularly. Blood samples were collected prior to immunization (V1) and 1-2 months (V2), 1 year after immunization (V3) and 1-2 after the booster dose (V4). The bactericidal assay and ELISA was used to evaluate the bactericidal antibody response and IgG response to C polysaccharide, respectively. Two doses of vaccine were required seroconversion of most (70%) of the subjects. There was a marked decrease of bactericidal antibodies and specific IgG one year after the first dose of vaccine. We observed a positive and significant correlations between the results obtained in the bactericidal assay and ELISA after 2 doses of vaccine. The same was seen when we analyze the correlation between the frequency of seroconversion assessed by two tests. We observed a significant correlation between IgG anti-C polysaccharide and SBA titers after the first dose of the vaccine only fort s N79/96, suggesting the influence of the ortho-acetyl radical (OAc), since the C polysaccharide of N79/96 strain is OAc+ while N753/00 is OAc-. This study demonstrated that one dose of vaccine induced immune memory in patients HIV+, which was activated by the second dose of vaccine, which was essential for the seroconversion of most of the patients. It remains important to investigate the best period for the second dose, so that we could have an even greater number of responders in this group of patients.