Biocompatibilidade do polissacarídeo celulósico sintetizado por zoogloea sp.: um estudo em bexigas de coelho

• Autor(a) principal: Gomes, Henrique Lima
• Data de Publicação: 2012
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/12353
• topic: Bipolymer; Deflux; Zoogloea sp; Cellulosic exopolysaccharide; Bexiga; Polissacarideos; Biopolímeros; Coelho como animal de laboratório; Zoogloea sp; Materiais biocompatíveis; Deflux; Exopolissacarídeo celulósico; CNPQ::CIENCIAS DA SAUDE

Actually the material used for the endoscopic treatment is the Deflux, however this is a non-biological material. It is known that the ideal substance should be non-toxic, biocompatible, non-antigenic, non-migratory and must cause the least possible inflammation. Zoogloea SP. bacteria produces a cellulosic exopolysaccharide (CEP) with low cytotoxicity and high biocompatibility.The aim of this study is to investigate, in rabbit bladder, the biocompatibility of CEP implants produced by Zooglea sp.Were used as experimental model, 20 adult rabbits California race, with an average of six months of age. The animals were divided into two groups, being the G1 group, composed of fallen three days after application of the implant (n=9), and the Group G2, composed of animals dead three months after the application of implant (n=11). Each animal received, in total, four implants, being two of biopolymer gel and two of Deflux gel. Imunohistochemistry techniques were performed for marking of collagen types I and III, Alpha-smooth muscle actin, PCNA and chemical reaction tunnel.In samples of three days, the CEP and deflux implants were structurally homogenous and free of inflammatory cells or blood vessels. On the other hand, in samples of three months, with the exception of a few areas, the CEP was organized as short beams that were suggestive of a fibrous tissue. Despite this, the implant of CEP blushed adversely to collagen type I and III, elastic fibers, while the masson, not indicated the presence of collagen. In contrast the areas of deflux implant in samples of three months were fragmented, but were still homogeneous, and still there was no cell or blood vessel in his heart. PCNA positive cells could be clearly seen inside these islets, thus indicating a proliferative-inflammatory process ongoing. In three months, group sacrificed that received deflux implants were still negative, but around the areas of CEP some cells positive for the technique of TUNEL were seen. In the CEP implants carried out three months apart, many blood vessels were viewed, and its density was of 23.86 ± 5.48. The microvascular density in the bladder wall (41.51 ± 11.19) was significantly different (p 0.001) of that in the CEP implant.Our results showed that the CEP has low immunogenicity and integrates better in host tissue when compared to deflux. So CEP must be an efficient material in cases where the incorporation to the tissue is desired such as support structures in reconstructive surgery