Expressão de fatores apoptóticos em biópsias de tecido gengival de pacientes com periodontite crônica severa generalizada

• Autor(a) principal: Alves, Juliana Cardoso
• Data de Publicação: 2016
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/14214
• topic: Periodontitis; Apoptosis; Gingival tissue Biopsy; Gingivitis; Periodontite; Apoptose; Gengivas - Biópsia; Gengivite; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA

The aim of this study was to evaluate the expression of the apoptotic markers caspase-3, survivin and the dimers Bcl-xL/Bak and Mcl-1/Bak, in gingival tissue biopsies in subjects having severe Generalized Chronic Periodontitis (GCP). In the test group were assessed 15 subjects affected by severe GCP with mean age of 54,7 + 8,4 years. The control group was formed with 10 subjects with gingivitis with mean age of 38,9 + 5,4 years. All teeth in the patients were evaluated (except the third molars) for Pocket Depth (PD), Attachment Level (AL), Visible Plaque Index (VPI) and Gingival Bleeding Index (GBI). In the teeth where the biopsies were collected, Plaque Index (PI) and Gingival Index (GI) were also measured. Two biopsies in the test group and one biopsy in the control group were collected using a 1,5 mm of diameter punch and stored at -70º Celsius (C). Just before analysis, the samples were weighted and macerated with a cell disrupter. Each biopsy was divided into cytosolic and nuclear/mitochondrial fractions. These fractions were analyzed in a multiplex microsphere immunoassay and the expression of the apoptotic markers caspase-3, survivin and the dimers Bcl-xL/Bak and Mcl-1/Bak were shown in nanogram per milligram of tissue (ng/mg). The data were presented in median and interquartile range and measured using the Mann-Whitney U Test with p < 0,05. Spearman s correlation was deemed relevant when r > 0,6 with p < 0,01. The values of PD, AL and GBI were significantly higher in the test group, when compared to the control (p < 0,001, p < 0,001, p = 0,035, respectively). The group of pacients suffering with periodontitis presented, in the nuclear/mitochondrial fraction, concentrations of Mcl-1/Bak and survivin significantly higher when compared to the control (p = 0,05 for both). The correlation between the caspase-3 and the Bcl-xL/Bak was significantly positive, from both the cytosolic and the nuclear/mitochondrial fractions (p < 0,001, p = 0,003, respectively). We conclude that the subjects having periodontitis presented significantly higher levels of the antiapoptotic markers survivin and the dimer Mcl-1/Bak, in the nuclear/mitochondrial fraction, suggesting a possible retarding effect on the process of apoptosis.