Ação antimicrobiana, propriedades mecânicas e citotoxicidade de um polímero desenvolvido a partir da adição de clorexidina e quaternário de amônio

• Autor(a) principal: Campos, Karina de Paula Lopes
• Data de Publicação: 2018
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/14233
• topic: PMMA; Chlorhexidine; Quaternary of ammonium; Biofilm inhibition; Mechanical properties; Color stability; Cytotoxicity; PMMA; Clorexidina; Quaternário de amônio; Inibição de biofilme; Propriedades mecânicas; Estabilidade de cor; Citotoxicidade; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA

The aim of this study was investigate antimicrobial, mechanical and physical properties, and cytotoxicity of a polymer incorporated with dimethylaminohexadecyl methacrylate (DMAHDM) and chlorhexidine (dCHX), isolated and associated. Thus, 132 disk-shaped specimens (108 in 8-mm x 2-mm and 24 in 5-mm x 2-mm) and 48 rectangular specimens (65 x 10 x 3.4 mm ISO 20795-1:2013) were divided into four groups according to the DMAHDM and dCHX incorporations: 0% (control), 1% dCHX, 5% DMAHDM and 1% dCHX +5% DMAHDM associated. Biofilm viability, flexural strength (FS), surface roughness (SR) and color stability (ΔE) were analyzed after 48 h of storage in distilled water at 37°C (initial). Cytotoxicity was measured after 24h, 3 and 7 days of elution by MTT test on L929 cells (ISO 10993-5:2009). FS was measured by the three-point bending test in a universal testing machine. SR and ΔE were tested by a contact profilometer and a portable spectrophotometer, respectively, after 4 weeks of storage in distilled water at 37ºC and after 72h of immersion in coffee. Data were submitted to ANOVA and Bonferroni/Tukey tests (p&#8804;0,05). All the tested groups showed significant antimicrobial activity against Streptoccocus mutans and Candida albicans, when compared to control group (p<0,05). Only the dCHX group, at 24 h of elution, did not demonstrate cytotoxic effects (lower than 30% of reduction in cells viability). There was a reduction for FS on tested groups (p<0,05) compared to control group. No differences were detected in initial roughness measurements among tested groups (p>0,05). However, after storage and immersion in coffee, the DMAHDM s groups showed rougher surfaces and significant color changes compared to control (p<0,05). The results of this study suggests that polymers with dCHX and DMAHDM incorporated presents antimicrobial properties, but their mechanical and physical properties and the cytotoxicity are compromised