Efeito do extrato de Euterpe oleracea Mart. (açaí) sobre as alterações renais em animais com hipertensão espontânea associada ao diabetes mellitus do tipo 1
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Tipo de documento: | Tese |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UERJ |
Texto Completo: | http://www.bdtd.uerj.br/handle/1/12684 |
Resumo: | The coexistence of high blood pressure (hypertension) and diabetes mellitus (DM) significantly increases the risk of cardiovascular and renal events, especially nephropathy and its complications. Increasing evidence highlights the central role of oxidative stress and inflammation in cardiovascular and renal disorders associated with diabetes and hypertension. The hydroalcoholic extract of the seed of Euterpe oleracea Mart. (ASE), popularly known as acai is a potent vasodilator with antihypertensive and antioxidant actions. In the present study, we evaluated the effects of chronic treatment with ASE in preventing the development of renal functional and structural changes in an experimental model of hypertension associated with DM induced by streptozotocin (STZ). Male Wistar normotensive and spontaneously hypertensive rats (SHR) of 10 weeks old received an intraperitoneal injection of 50 mg kg-1 of STZ (D and DH groups) or vehicle (C and H groups) and then treatment with ASE (200 mg/ kg / day) was performed for 45 days in D (D+ASE) and DH animals (DH+ASE). ASE prevented the increase in systolic blood pressure in D group and decreased in DH. The vasodilator response to ACh was reduced in D, H and HD vs C (p <0.05), and ASE prevented endothelial dysfunction in all groups (p <0.05). ASE decreased plasma renin levels that were higher in group H vs C, as well as immunostaining of renin in the kidney which was higher in D and H compared to C. No further changes were observed in DH group. The increased serum levels of glucose, insulin, HbA1c, TG, and VLDL in D and DH (p <0.05) were significantly reduced by ASE. The levels of creatinine and urea in serum and urine, as well as microalbuminuria and total protein excretion were higher in DH compared to H. The same was observed in D compared to C, with the exception of urinary urea that remained unchanged. All these serum and renal parameters were reduced by the ASE in D and DH groups. Plasma levels of fibrinogen were higher in D and H compared to C, and no further increase was observed in DH. ASE reduced these levels in D and DH. The reduction in the number of glomeruli in DH; the increase of TGF-β1 and collagen IV deposition; the reduction of nephrin and podocin expression and the increase of caspase-3 were prevented by ASE (p <0.05). The serum levels and renal expression of IL-6, TNF-α and MCP-1 were higher in D and DH compared to C and H groups and were reduced by treatment with ASE (p <0.05). The increased levels of malondialdehyde, carbonyl and 8-isoprostrane in the kidneys of DH and D were reduced by ASE (p <0.05). The activities of antioxidant enzymes were shown to be reduced in DH and D, and were increased by ASE. The prevention of renal oxidative damage in DH promoted by ASE, correlated with the prevention of increased expression of pro-oxidant enzymes Nox4, gp91 and p47; with the increased activity of antioxidant enzymes and nitrite levels, as well as the reduction of iNOS expression in the kidney of the animals. We conclude that treatment with ASE promoted antihypertensive effect and improved renal function and structure by its vasodilator, anti-inflammatory and antioxidant actions. |
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Resende, Angela de Castrohttp://lattes.cnpq.br/2483198584037482Moura, Roberto Soares dehttp://lattes.cnpq.br/5248304930462161Silva, Cláudia Lúcia Martins dahttp://lattes.cnpq.br/6943489346365796Valença, Samuel dos Santoshttp://lattes.cnpq.br/3979148859159035Coelho, Marsen Garcia Pintohttp://lattes.cnpq.br/4468352500732645Daleprane, Júlio Beltramehttp://lattes.cnpq.br/6027048461430935http://lattes.cnpq.br/0987768129012563Cordeiro, Viviane da Silva Cristino2021-01-06T20:54:37Z2018-05-212016-02-24CORDEIRO, Viviane da Silva Cristino. Efeito do extrato de Euterpe oleracea Mart. (açaí) sobre as alterações renais em animais com hipertensão espontânea associada ao diabetes mellitus do tipo 1. 2016. 19 f. Tese (Doutorado em Fisiopatologia Clínica e Experimental) - Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2016.http://www.bdtd.uerj.br/handle/1/12684The coexistence of high blood pressure (hypertension) and diabetes mellitus (DM) significantly increases the risk of cardiovascular and renal events, especially nephropathy and its complications. Increasing evidence highlights the central role of oxidative stress and inflammation in cardiovascular and renal disorders associated with diabetes and hypertension. The hydroalcoholic extract of the seed of Euterpe oleracea Mart. (ASE), popularly known as acai is a potent vasodilator with antihypertensive and antioxidant actions. In the present study, we evaluated the effects of chronic treatment with ASE in preventing the development of renal functional and structural changes in an experimental model of hypertension associated with DM induced by streptozotocin (STZ). Male Wistar normotensive and spontaneously hypertensive rats (SHR) of 10 weeks old received an intraperitoneal injection of 50 mg kg-1 of STZ (D and DH groups) or vehicle (C and H groups) and then treatment with ASE (200 mg/ kg / day) was performed for 45 days in D (D+ASE) and DH animals (DH+ASE). ASE prevented the increase in systolic blood pressure in D group and decreased in DH. The vasodilator response to ACh was reduced in D, H and HD vs C (p <0.05), and ASE prevented endothelial dysfunction in all groups (p <0.05). ASE decreased plasma renin levels that were higher in group H vs C, as well as immunostaining of renin in the kidney which was higher in D and H compared to C. No further changes were observed in DH group. The increased serum levels of glucose, insulin, HbA1c, TG, and VLDL in D and DH (p <0.05) were significantly reduced by ASE. The levels of creatinine and urea in serum and urine, as well as microalbuminuria and total protein excretion were higher in DH compared to H. The same was observed in D compared to C, with the exception of urinary urea that remained unchanged. All these serum and renal parameters were reduced by the ASE in D and DH groups. Plasma levels of fibrinogen were higher in D and H compared to C, and no further increase was observed in DH. ASE reduced these levels in D and DH. The reduction in the number of glomeruli in DH; the increase of TGF-β1 and collagen IV deposition; the reduction of nephrin and podocin expression and the increase of caspase-3 were prevented by ASE (p <0.05). The serum levels and renal expression of IL-6, TNF-α and MCP-1 were higher in D and DH compared to C and H groups and were reduced by treatment with ASE (p <0.05). The increased levels of malondialdehyde, carbonyl and 8-isoprostrane in the kidneys of DH and D were reduced by ASE (p <0.05). The activities of antioxidant enzymes were shown to be reduced in DH and D, and were increased by ASE. The prevention of renal oxidative damage in DH promoted by ASE, correlated with the prevention of increased expression of pro-oxidant enzymes Nox4, gp91 and p47; with the increased activity of antioxidant enzymes and nitrite levels, as well as the reduction of iNOS expression in the kidney of the animals. We conclude that treatment with ASE promoted antihypertensive effect and improved renal function and structure by its vasodilator, anti-inflammatory and antioxidant actions.A coexistência mais frequente da hipertensão e diabetes mellitus (DM) aumenta significativamente o risco de eventos cardiovasculares e renais, especialmente, a nefropatia e suas complicações. Evidências crescentes destacam o papel central do estresse oxidativo e inflamação nas alterações cardiovasculares e renais associadas a estas doenças. O extrato hidroalcoólico da semente do fruto de Euterpe oleracea Mart. (ASE), popularmente conhecida como açaí, é um potente vasodilatador com ação anti-hipertensiva e antioxidante. Neste estudo, avaliamos os efeitos do tratamento com ASE na prevenção do desenvolvimento de alterações funcionais e estruturais renais no modelo experimental de hipertensão associada ao DM induzido por estreptozotocina (STZ). Ratos Wistar normotensos e espontaneamente hipertensos (SHR) com 10 semanas de idade, receberam injeção intraperitoneal de 50 mg kg-1 de STZ (grupos D e HD) ou veículo (grupos C e H) e tratados com ASE (200 mg/kg/dia, v.o) durante 45 dias nos grupos D (D+ASE) e HD (HD+ASE). A pressão arterial sistólica foi maior nos H vs C, D vs C e HD vs H. O ASE preveniu o aumento da PAS no grupo D e reduziu no HD. A resposta vasodilatadora à ACh foi menor em D, H e HD vs C (p<0.05), e o ASE preveniu a disfunção endotelial em todos os grupos (p<0.05). O ASE reduziu os níveis plasmáticos de renina que foram maiores no grupo H vs C, assim como, a imunomarcação em rim que foi maior em D e H comparados ao C, sem alteração adicional no grupo HD. Os níveis séricos de glicose, insulina, HbA1c, TG e VLDL foram maiores nos grupos D e HD (p<0.05), e foram significativamente reduzidos pelo ASE. Os níveis de creatinina e ureia (sérica e urinária), a microalbuminúria e excreção de proteínas totais foram maiores no grupo HD comparados ao H, igualmente em D comparado ao C, exceto a ureia urinária que permaneceu inalterada. Todos os parâmetros séricos e renais foram reduzidos pelo ASE em D e HD. A redução no número de glomérulos em HD; o aumento do TGF-β1 e deposição de colágeno IV; a redução da expressão de nefrina e podocina e o aumento da caspase-3 foram prevenidos pelo ASE (p<0.05). Os níveis séricos e a expressão renal de IL-6, TNF-α e MCP-1 apresentaram-se maiores em D e HD comparados aos grupos C e H e foram reduzidos pelo tratamento com ASE (p<0.05). Os níveis de malondialdeído, carbonil e 8-isoprostrano foram maiores em rins de D e HD e reduzidos pelo ASE (p<0.05). As atividades das enzimas antioxidantes mostraram-se reduzidas nos D e HD e foram aumentadas pelo ASE. A prevenção do dano oxidativo renal em HD promovida por ASE, correlacionou com a prevenção do aumento de NOX4, gp91e p47; com o aumento da atividade das enzimas antioxidantes e dos níveis de nitrito, assim como, a redução da expressão de iNOS no rim desses animais. Concluímos que o tratamento com ASE promoveu efeito anti-hipertensivo e de melhora da função e estrutura renal pela sua ação vasodilatadora, anti-inflamatória e antioxidante.Submitted by Boris Flegr (boris@uerj.br) on 2021-01-06T20:54:37Z No. of bitstreams: 1 Viviane da Silva Cristino Cordeiro Tese completa.pdf: 347871 bytes, checksum: 4330c5b7e84ed04eff358bbeb62d24f1 (MD5)Made available in DSpace on 2021-01-06T20:54:37Z (GMT). No. of bitstreams: 1 Viviane da Silva Cristino Cordeiro Tese completa.pdf: 347871 bytes, checksum: 4330c5b7e84ed04eff358bbeb62d24f1 (MD5) Previous issue date: 2016-02-24Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade do Estado do Rio de JaneiroPrograma de Pós-Graduação em Fisiopatologia Clínica e ExperimentalUERJBRCentro Biomédico::Faculdade de Ciências MédicasHypertensionDiabetesStreptozotocinSHR. Endothelial dysfunctionOxidative stressEuterpe oleracea MartDiabetic nephropathyHipertensãoDiabetesEstreptozotocinaSHRDisfunção endotelialEstresse oxidativoEuterpe oleracea MartNefropatia diabéticaAçaí Uso terapêuticoHipertensãoDiabetes mellitusRatos endogâmicos SHREndotélioEstresse oxidativoNefropatias diabéticasCNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA::FARMACOLOGIA CARDIORENALEfeito do extrato de Euterpe oleracea Mart. (açaí) sobre as alterações renais em animais com hipertensão espontânea associada ao diabetes mellitus do tipo 1Effect of the Euterpe oleracea Mart. extract (açaí) on renal disorders in animals with spontaneous hypertension associated with diabetes mellitus type 1info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/doctoralThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UERJinstname:Universidade do Estado do Rio de Janeiro (UERJ)instacron:UERJORIGINALViviane da Silva Cristino Cordeiro Tese completa.pdfapplication/pdf347871http://www.bdtd.uerj.br/bitstream/1/12684/1/Viviane+da+Silva+Cristino+Cordeiro+Tese+completa.pdf4330c5b7e84ed04eff358bbeb62d24f1MD511/126842024-02-26 16:36:38.544oai:www.bdtd.uerj.br:1/12684Biblioteca Digital de Teses e Dissertaçõeshttp://www.bdtd.uerj.br/PUBhttps://www.bdtd.uerj.br:8443/oai/requestbdtd.suporte@uerj.bropendoar:29032024-02-26T19:36:38Biblioteca Digital de Teses e Dissertações da UERJ - Universidade do Estado do Rio de Janeiro (UERJ)false |
dc.title.por.fl_str_mv |
Efeito do extrato de Euterpe oleracea Mart. (açaí) sobre as alterações renais em animais com hipertensão espontânea associada ao diabetes mellitus do tipo 1 |
dc.title.alternative.eng.fl_str_mv |
Effect of the Euterpe oleracea Mart. extract (açaí) on renal disorders in animals with spontaneous hypertension associated with diabetes mellitus type 1 |
title |
Efeito do extrato de Euterpe oleracea Mart. (açaí) sobre as alterações renais em animais com hipertensão espontânea associada ao diabetes mellitus do tipo 1 |
spellingShingle |
Efeito do extrato de Euterpe oleracea Mart. (açaí) sobre as alterações renais em animais com hipertensão espontânea associada ao diabetes mellitus do tipo 1 Cordeiro, Viviane da Silva Cristino Hypertension Diabetes Streptozotocin SHR. Endothelial dysfunction Oxidative stress Euterpe oleracea Mart Diabetic nephropathy Hipertensão Diabetes Estreptozotocina SHR Disfunção endotelial Estresse oxidativo Euterpe oleracea Mart Nefropatia diabética Açaí Uso terapêutico Hipertensão Diabetes mellitus Ratos endogâmicos SHR Endotélio Estresse oxidativo Nefropatias diabéticas CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA::FARMACOLOGIA CARDIORENAL |
title_short |
Efeito do extrato de Euterpe oleracea Mart. (açaí) sobre as alterações renais em animais com hipertensão espontânea associada ao diabetes mellitus do tipo 1 |
title_full |
Efeito do extrato de Euterpe oleracea Mart. (açaí) sobre as alterações renais em animais com hipertensão espontânea associada ao diabetes mellitus do tipo 1 |
title_fullStr |
Efeito do extrato de Euterpe oleracea Mart. (açaí) sobre as alterações renais em animais com hipertensão espontânea associada ao diabetes mellitus do tipo 1 |
title_full_unstemmed |
Efeito do extrato de Euterpe oleracea Mart. (açaí) sobre as alterações renais em animais com hipertensão espontânea associada ao diabetes mellitus do tipo 1 |
title_sort |
Efeito do extrato de Euterpe oleracea Mart. (açaí) sobre as alterações renais em animais com hipertensão espontânea associada ao diabetes mellitus do tipo 1 |
author |
Cordeiro, Viviane da Silva Cristino |
author_facet |
Cordeiro, Viviane da Silva Cristino |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Resende, Angela de Castro |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/2483198584037482 |
dc.contributor.advisor-co1.fl_str_mv |
Moura, Roberto Soares de |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/5248304930462161 |
dc.contributor.referee1.fl_str_mv |
Silva, Cláudia Lúcia Martins da |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/6943489346365796 |
dc.contributor.referee2.fl_str_mv |
Valença, Samuel dos Santos |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/3979148859159035 |
dc.contributor.referee3.fl_str_mv |
Coelho, Marsen Garcia Pinto |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/4468352500732645 |
dc.contributor.referee4.fl_str_mv |
Daleprane, Júlio Beltrame |
dc.contributor.referee4Lattes.fl_str_mv |
http://lattes.cnpq.br/6027048461430935 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/0987768129012563 |
dc.contributor.author.fl_str_mv |
Cordeiro, Viviane da Silva Cristino |
contributor_str_mv |
Resende, Angela de Castro Moura, Roberto Soares de Silva, Cláudia Lúcia Martins da Valença, Samuel dos Santos Coelho, Marsen Garcia Pinto Daleprane, Júlio Beltrame |
dc.subject.eng.fl_str_mv |
Hypertension Diabetes Streptozotocin SHR. Endothelial dysfunction Oxidative stress Euterpe oleracea Mart Diabetic nephropathy |
topic |
Hypertension Diabetes Streptozotocin SHR. Endothelial dysfunction Oxidative stress Euterpe oleracea Mart Diabetic nephropathy Hipertensão Diabetes Estreptozotocina SHR Disfunção endotelial Estresse oxidativo Euterpe oleracea Mart Nefropatia diabética Açaí Uso terapêutico Hipertensão Diabetes mellitus Ratos endogâmicos SHR Endotélio Estresse oxidativo Nefropatias diabéticas CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA::FARMACOLOGIA CARDIORENAL |
dc.subject.por.fl_str_mv |
Hipertensão Diabetes Estreptozotocina SHR Disfunção endotelial Estresse oxidativo Euterpe oleracea Mart Nefropatia diabética Açaí Uso terapêutico Hipertensão Diabetes mellitus Ratos endogâmicos SHR Endotélio Estresse oxidativo Nefropatias diabéticas |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::FARMACOLOGIA::FARMACOLOGIA CARDIORENAL |
description |
The coexistence of high blood pressure (hypertension) and diabetes mellitus (DM) significantly increases the risk of cardiovascular and renal events, especially nephropathy and its complications. Increasing evidence highlights the central role of oxidative stress and inflammation in cardiovascular and renal disorders associated with diabetes and hypertension. The hydroalcoholic extract of the seed of Euterpe oleracea Mart. (ASE), popularly known as acai is a potent vasodilator with antihypertensive and antioxidant actions. In the present study, we evaluated the effects of chronic treatment with ASE in preventing the development of renal functional and structural changes in an experimental model of hypertension associated with DM induced by streptozotocin (STZ). Male Wistar normotensive and spontaneously hypertensive rats (SHR) of 10 weeks old received an intraperitoneal injection of 50 mg kg-1 of STZ (D and DH groups) or vehicle (C and H groups) and then treatment with ASE (200 mg/ kg / day) was performed for 45 days in D (D+ASE) and DH animals (DH+ASE). ASE prevented the increase in systolic blood pressure in D group and decreased in DH. The vasodilator response to ACh was reduced in D, H and HD vs C (p <0.05), and ASE prevented endothelial dysfunction in all groups (p <0.05). ASE decreased plasma renin levels that were higher in group H vs C, as well as immunostaining of renin in the kidney which was higher in D and H compared to C. No further changes were observed in DH group. The increased serum levels of glucose, insulin, HbA1c, TG, and VLDL in D and DH (p <0.05) were significantly reduced by ASE. The levels of creatinine and urea in serum and urine, as well as microalbuminuria and total protein excretion were higher in DH compared to H. The same was observed in D compared to C, with the exception of urinary urea that remained unchanged. All these serum and renal parameters were reduced by the ASE in D and DH groups. Plasma levels of fibrinogen were higher in D and H compared to C, and no further increase was observed in DH. ASE reduced these levels in D and DH. The reduction in the number of glomeruli in DH; the increase of TGF-β1 and collagen IV deposition; the reduction of nephrin and podocin expression and the increase of caspase-3 were prevented by ASE (p <0.05). The serum levels and renal expression of IL-6, TNF-α and MCP-1 were higher in D and DH compared to C and H groups and were reduced by treatment with ASE (p <0.05). The increased levels of malondialdehyde, carbonyl and 8-isoprostrane in the kidneys of DH and D were reduced by ASE (p <0.05). The activities of antioxidant enzymes were shown to be reduced in DH and D, and were increased by ASE. The prevention of renal oxidative damage in DH promoted by ASE, correlated with the prevention of increased expression of pro-oxidant enzymes Nox4, gp91 and p47; with the increased activity of antioxidant enzymes and nitrite levels, as well as the reduction of iNOS expression in the kidney of the animals. We conclude that treatment with ASE promoted antihypertensive effect and improved renal function and structure by its vasodilator, anti-inflammatory and antioxidant actions. |
publishDate |
2016 |
dc.date.issued.fl_str_mv |
2016-02-24 |
dc.date.available.fl_str_mv |
2018-05-21 |
dc.date.accessioned.fl_str_mv |
2021-01-06T20:54:37Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/doctoralThesis |
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doctoralThesis |
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dc.identifier.citation.fl_str_mv |
CORDEIRO, Viviane da Silva Cristino. Efeito do extrato de Euterpe oleracea Mart. (açaí) sobre as alterações renais em animais com hipertensão espontânea associada ao diabetes mellitus do tipo 1. 2016. 19 f. Tese (Doutorado em Fisiopatologia Clínica e Experimental) - Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2016. |
dc.identifier.uri.fl_str_mv |
http://www.bdtd.uerj.br/handle/1/12684 |
identifier_str_mv |
CORDEIRO, Viviane da Silva Cristino. Efeito do extrato de Euterpe oleracea Mart. (açaí) sobre as alterações renais em animais com hipertensão espontânea associada ao diabetes mellitus do tipo 1. 2016. 19 f. Tese (Doutorado em Fisiopatologia Clínica e Experimental) - Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2016. |
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http://www.bdtd.uerj.br/handle/1/12684 |
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Universidade do Estado do Rio de Janeiro |
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Programa de Pós-Graduação em Fisiopatologia Clínica e Experimental |
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UERJ |
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Centro Biomédico::Faculdade de Ciências Médicas |
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Universidade do Estado do Rio de Janeiro |
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