Modulação da adipogênese pelo extrato do caroço de açaí em adipócitos 3T3-L1
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UERJ |
Texto Completo: | http://www.bdtd.uerj.br/handle/1/17261 |
Resumo: | Body adiposity is an important risk factor for the development of chronic non-communicable diseases (NCD). Studies on the process of adipogenesis have been extensively performed in in vitro models, to describe the molecular and cellular bases of adipose tissue development, thus allowing the formulation of therapeutic and preventive strategies of obesity. The açai seed extract (ASE) has been evidenced as a potential regulator of body mass, acting in reduction of the content of adipose tissue in experimental models of obesity. In this way, the aim of this study was to evaluate the effects of ASE on the modulation of adipogenesis. To perform the work, 3T3-L1 cells were differentiated into adipocytes. The cells were incubated with different concentrations of ASE in the previous period (Pretreatment) to induce cell differentiation and after it (Treatment). Tests of viability, accumulation of intracellular triglycerides, expression of proteins involved in lipid metabolism and cell death were performed. The results showed that the ASE exposure, in a dose dependent manner, led to a decrease (P<0.05) in proliferation and cell viability, inhibited (P<0.05) differentiation through the decreasing expression of transcription factors and adipogenic proteins (PPARɣ, SREBP-1 and FAS), in addition to promoting increased expression of P53 protein and cause cell death mediated by apoptosis and necrosis. Taken together, the results show the performance of the phenolic compounds present in ASE and reinforce, although in in vitro assays, the potential of this extract as a possible modulation factor of adipogenesis. |
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Daleprane, Julio Beltramehttp://lattes.cnpq.br/6027048461430935Costa, Danielly Cristiny Ferraz dahttp://lattes.cnpq.br/9617293991265023Leandro, Carol Virgínia Goishttp://lattes.cnpq.br/7419672108203411Reis, Marta Citelli doshttp://lattes.cnpq.br/1136248028072284http://lattes.cnpq.br/3325473045705174Trindade, Patricia Leticia2022-03-14T14:03:29Z2017-07-28TRINDADE, Patricia Leticia. Modulação da adipogênese pelo extrato do caroço de açaí em adipócitos 3T3-L1. 2017. 61 f. Dissertação (Mestrado em Alimentação, Nutrição e Saúde) – Instituto de Nutrição, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2017.http://www.bdtd.uerj.br/handle/1/17261Body adiposity is an important risk factor for the development of chronic non-communicable diseases (NCD). Studies on the process of adipogenesis have been extensively performed in in vitro models, to describe the molecular and cellular bases of adipose tissue development, thus allowing the formulation of therapeutic and preventive strategies of obesity. The açai seed extract (ASE) has been evidenced as a potential regulator of body mass, acting in reduction of the content of adipose tissue in experimental models of obesity. In this way, the aim of this study was to evaluate the effects of ASE on the modulation of adipogenesis. To perform the work, 3T3-L1 cells were differentiated into adipocytes. The cells were incubated with different concentrations of ASE in the previous period (Pretreatment) to induce cell differentiation and after it (Treatment). Tests of viability, accumulation of intracellular triglycerides, expression of proteins involved in lipid metabolism and cell death were performed. The results showed that the ASE exposure, in a dose dependent manner, led to a decrease (P<0.05) in proliferation and cell viability, inhibited (P<0.05) differentiation through the decreasing expression of transcription factors and adipogenic proteins (PPARɣ, SREBP-1 and FAS), in addition to promoting increased expression of P53 protein and cause cell death mediated by apoptosis and necrosis. Taken together, the results show the performance of the phenolic compounds present in ASE and reinforce, although in in vitro assays, the potential of this extract as a possible modulation factor of adipogenesis.A adiposidade corporal é um importante fator de risco para o desenvolvimento de doenças crônicas não transmissíveis (DCNT). Estudos sobre o processo de adipogênese têm sido extensivamente realizados em modelos in vitro, com o intuito de descrever as bases moleculares e celulares do desenvolvimento do tecido adiposo, permitindo assim a formulação de estratégias terapêuticas e preventivas da obesidade. O extrato hidroalcóolico do açaí (ASE) tem sido evidenciado como potencial regulador da massa corporal, agindo na redução do conteúdo de tecido adiposo em modelos experimentais de obesidade. Desta forma, o objetivo deste estudo foi avaliar o efeito do ASE na modulação da adipogênese. Para realização do trabalho, células 3T3-L1 foram diferenciadas a adipócitos. As células foram incubadas com diferentes concentrações de ASE no período prévio (Pré-tratamento) à indução da diferenciação celular e após o mesmo (Tratamento). Foram realizados ensaios de viabilidade, acúmulo de triglicerídeos intracelulares, expressão de proteínas envolvidas no metabolismo lipídico e morte celular. Os resultados obtidos demonstraram que a exposição ao ASE, de forma dose dependente, levou a uma diminuição (P<0,05) da proliferação e viabilidade celular, inibiu (p<0,05) a diferenciação por meio da diminuição da expressão de fatores de transcrição e proteínas adipogênicas (PPARɣ, SREBP-1 e FAS), além de promover aumento da expressão da proteína P53 e provocar morte celular mediada por apoptose e necrose. Em conjunto, os resultados obtidos expõem a atuação dos compostos fenólicos presentes no ASE e reforça, embora que em ensaios in vitro, o potencial deste extrato como um possível fator de modulação da adipogênese.Submitted by Mariangela CEH/A (marianfig@gmail.com) on 2022-03-14T14:03:29Z No. of bitstreams: 2 Tese - Elaine dos Ramos Soares - 2020 - Completa.pdf: 1620634 bytes, checksum: 1caa4628bfedbb690f5847ae9a4d0b6c (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5)Made available in DSpace on 2022-03-14T14:03:29Z (GMT). No. of bitstreams: 2 Tese - Elaine dos Ramos Soares - 2020 - Completa.pdf: 1620634 bytes, checksum: 1caa4628bfedbb690f5847ae9a4d0b6c (MD5) license_rdf: 0 bytes, checksum: d41d8cd98f00b204e9800998ecf8427e (MD5) Previous issue date: 2017-07-28application/pdfporUniversidade do Estado do Rio de JaneiroPrograma de Pós-Graduação em Alimentação, Nutrição e SaúdeUERJBrasilCentro Biomédico::Instituto de Nutriçãohttp://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessPhenolic CompoundsAdipogenesisObesityNutriçãoAçaíObesidadeCompostos fenólicosAdipogênese3T3-L1CIENCIAS DA SAUDE::NUTRICAOModulação da adipogênese pelo extrato do caroço de açaí em adipócitos 3T3-L1Modulation of adipogenesis by açai seed extract in 3T3-L1 adipocytesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisreponame:Biblioteca Digital de Teses e Dissertações da UERJinstname:Universidade do Estado do Rio de Janeiro (UERJ)instacron:UERJORIGINALDissertação - Patricia Leticia Trindade - 2017 - Completa.pdfDissertação - Patricia Leticia Trindade - 2017 - Completa.pdfapplication/pdf2123513http://www.bdtd.uerj.br/bitstream/1/17261/6/Disserta%C3%A7%C3%A3o+-+Patricia+Leticia+Trindade+-+2017+-+Completa.pdff7e6ad559dec671b8422b925bbed2cf5MD56CC-LICENSElicense_urllicense_urltext/plain; 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dc.title.por.fl_str_mv |
Modulação da adipogênese pelo extrato do caroço de açaí em adipócitos 3T3-L1 |
dc.title.alternative.eng.fl_str_mv |
Modulation of adipogenesis by açai seed extract in 3T3-L1 adipocytes |
title |
Modulação da adipogênese pelo extrato do caroço de açaí em adipócitos 3T3-L1 |
spellingShingle |
Modulação da adipogênese pelo extrato do caroço de açaí em adipócitos 3T3-L1 Trindade, Patricia Leticia Phenolic Compounds Adipogenesis Obesity Nutrição Açaí Obesidade Compostos fenólicos Adipogênese 3T3-L1 CIENCIAS DA SAUDE::NUTRICAO |
title_short |
Modulação da adipogênese pelo extrato do caroço de açaí em adipócitos 3T3-L1 |
title_full |
Modulação da adipogênese pelo extrato do caroço de açaí em adipócitos 3T3-L1 |
title_fullStr |
Modulação da adipogênese pelo extrato do caroço de açaí em adipócitos 3T3-L1 |
title_full_unstemmed |
Modulação da adipogênese pelo extrato do caroço de açaí em adipócitos 3T3-L1 |
title_sort |
Modulação da adipogênese pelo extrato do caroço de açaí em adipócitos 3T3-L1 |
author |
Trindade, Patricia Leticia |
author_facet |
Trindade, Patricia Leticia |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Daleprane, Julio Beltrame |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/6027048461430935 |
dc.contributor.advisor-co1.fl_str_mv |
Costa, Danielly Cristiny Ferraz da |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/9617293991265023 |
dc.contributor.referee1.fl_str_mv |
Leandro, Carol Virgínia Gois |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/7419672108203411 |
dc.contributor.referee2.fl_str_mv |
Reis, Marta Citelli dos |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/1136248028072284 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/3325473045705174 |
dc.contributor.author.fl_str_mv |
Trindade, Patricia Leticia |
contributor_str_mv |
Daleprane, Julio Beltrame Costa, Danielly Cristiny Ferraz da Leandro, Carol Virgínia Gois Reis, Marta Citelli dos |
dc.subject.eng.fl_str_mv |
Phenolic Compounds Adipogenesis Obesity |
topic |
Phenolic Compounds Adipogenesis Obesity Nutrição Açaí Obesidade Compostos fenólicos Adipogênese 3T3-L1 CIENCIAS DA SAUDE::NUTRICAO |
dc.subject.por.fl_str_mv |
Nutrição Açaí Obesidade Compostos fenólicos Adipogênese 3T3-L1 |
dc.subject.cnpq.fl_str_mv |
CIENCIAS DA SAUDE::NUTRICAO |
description |
Body adiposity is an important risk factor for the development of chronic non-communicable diseases (NCD). Studies on the process of adipogenesis have been extensively performed in in vitro models, to describe the molecular and cellular bases of adipose tissue development, thus allowing the formulation of therapeutic and preventive strategies of obesity. The açai seed extract (ASE) has been evidenced as a potential regulator of body mass, acting in reduction of the content of adipose tissue in experimental models of obesity. In this way, the aim of this study was to evaluate the effects of ASE on the modulation of adipogenesis. To perform the work, 3T3-L1 cells were differentiated into adipocytes. The cells were incubated with different concentrations of ASE in the previous period (Pretreatment) to induce cell differentiation and after it (Treatment). Tests of viability, accumulation of intracellular triglycerides, expression of proteins involved in lipid metabolism and cell death were performed. The results showed that the ASE exposure, in a dose dependent manner, led to a decrease (P<0.05) in proliferation and cell viability, inhibited (P<0.05) differentiation through the decreasing expression of transcription factors and adipogenic proteins (PPARɣ, SREBP-1 and FAS), in addition to promoting increased expression of P53 protein and cause cell death mediated by apoptosis and necrosis. Taken together, the results show the performance of the phenolic compounds present in ASE and reinforce, although in in vitro assays, the potential of this extract as a possible modulation factor of adipogenesis. |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017-07-28 |
dc.date.accessioned.fl_str_mv |
2022-03-14T14:03:29Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/masterThesis |
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publishedVersion |
dc.identifier.citation.fl_str_mv |
TRINDADE, Patricia Leticia. Modulação da adipogênese pelo extrato do caroço de açaí em adipócitos 3T3-L1. 2017. 61 f. Dissertação (Mestrado em Alimentação, Nutrição e Saúde) – Instituto de Nutrição, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2017. |
dc.identifier.uri.fl_str_mv |
http://www.bdtd.uerj.br/handle/1/17261 |
identifier_str_mv |
TRINDADE, Patricia Leticia. Modulação da adipogênese pelo extrato do caroço de açaí em adipócitos 3T3-L1. 2017. 61 f. Dissertação (Mestrado em Alimentação, Nutrição e Saúde) – Instituto de Nutrição, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2017. |
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openAccess |
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Universidade do Estado do Rio de Janeiro |
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Programa de Pós-Graduação em Alimentação, Nutrição e Saúde |
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UERJ |
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Brasil |
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Centro Biomédico::Instituto de Nutrição |
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Universidade do Estado do Rio de Janeiro |
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