Análise da composição da matriz extracelular das camadas urotelial e muscular da bexiga de mulheres em diferentes idades

• Autor(a) principal: Brollo, Jorge Luiz Alves
• Data de Publicação: 2011
• Tipo de documento: Tese
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/12308
• topic: Bladder; Muscular layer; Collagen; Glycosaminoglycans; Urothelium; Bexiga; Camada muscular; Colágeno; Glicosaminoglicanos; Urotélio; CNPQ::CIENCIAS DA SAUDE::MEDICINA

Bladder compliance is dependent on smooth muscle, collagen fibers, elastic fiber and their ratios. The luminal surface of the urothelium is covered by an adhering glycosaminoglycan (GAG) layer. The aim of this study was to determine the composition of the extracellular matrix (ECM) in normal samples of women bladders through biochemistry analysis of collagen and GAG on samples obtained from individuals from different age groups, analyzing separately the urothelial and muscular layers. We studied samples taken from bladders of 17 patients divided in three different groups: childhood (N=5), menacme (N=6) and menopause (N=6). Bladders were analyzed for total GAG and collagen concentration per mg dry tissue and for the contents of GAG species, as determined by agarose electrophoresis and reported as the percent of total sulfated GAG. In muscular layer, collagen and GAG concentration showed no difference between groups. In urothelial layer, collagen concentration showed no difference between groups but GAG concentration in menopause ( 0.21 ± 0.12 μg hexuronic acid /mg dry tissue) was different from menacme (1.78 ± 1.62 μg hexuronic acid /mg dry tissue) and childhood ( 2.29 ± 1.32 μg hexuronic acid /mg dry tissue). There was no difference between sulfated GAG in three groups.In conclusion, GAG concentration in urothelial layer was substantially lower in menopause women.