A exposição a fumaça de cigarro altera distribuição de tecido adiposo branco de camundongos fêmeas jovens

• Autor(a) principal: Reis, Rejane Pontes Gaspar
• Data de Publicação: 2011
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/12734
• topic: Smoking; Adipose tissue; Abstinence; Fumo; Tecido adiposo; Abstinência; CNPQ::CIENCIAS BIOLOGICAS::FISIOLOGIA::FISIOLOGIA DE ORGAOS E SISTEMAS::FISIOLOGIA ENDOCRINA

Smoking and obesity are major causes of morbidity and mortality worldwide. Population studies have reported that smokers, especially women, have low body mass index. However, there are few studies that assess the body composition of humans and animals exposed to cigarette smoke, especially in teenagers. At 35 d of age (d) female mice were exposed to 3R4F cigarette smoke, with the average concentration of nicotine, 8 h / day for 15 days (group S= 12). Unexposed animals were evaluated in parallel (C= 12). Immediately after the expose half the animals of each group were sacrificed and the other half remained under observation for 30 d. During the experimental period, weight, body length and food intake were evaluated. At the end of the exposure period, animals were assessed by DEXA (Dual Energy X-ray Absorptiometry) and then sacrificed by exsanguination. For evaluation and confirmation of smoke exposure cotinine concentrations and lung morphology were evaluated. The serum cholesterol, triglycerides, glucose cotinine and insulin were measured. The samples of intra-abdominal and subcutaneous adipose tissue IA were collected and processed for morphological analysis. The expression of PPARγ, CPT1and UCP2 were measured in IA by western blotting. During exposure body mass, corporal length, intake, lean mass and mass IA, such of glucose, cholesterol and the expression of PPARγ did not differ .The expression UCP2 e CPT1, as circulating insulin decreased. The total body fat and trunk fat, triglycerides and cotinine increased. Morphological analysis revealed no change in IA tissue, but there was an increase and decrease in size of adipocytes in the SC tissue. During exposure the area and number of adipocytes IA were similar between groups, but decreased in SC in the area and increased number of adipocytes. After thirty days of abstinence, body mass, weight and number of adipocytes of IA tissue and glucose increased in group S, while decreased cholesterol, IA adipocyte area and number of SC. However, there were no chamber in the intake, the body length, lean mass, total fat mass and trunk, insulin and triglyceride levels and also the expression of PPARγ, UCP2 and CPT1 IA. Smoke exposure in young female mice triggered changes in adiposity, which have affected in a detrimental way metabolism. Even with the cessation of smoking, metabolic disorders remain significant