Diagnóstico sorológico da infecção pulmonar por Pseudomonas aeruginosa em crianças com Fibrose Cística

• Autor(a) principal: Cruz, Aline da Costa
• Data de Publicação: 2009
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/14418
• topic: Cystic Fibrosis; Lung Disease; P. aeruginosa; Type III Secretion System; Serological Diagnosis; Fibrose Cística; Doença Pulmonar; P. aeruginosa; Sistema de Secreção Tipo III; Diagnóstico Sorológico; CNPQ::CIENCIAS BIOLOGICAS::MICROBIOLOGIA

Cystic Fibrosis (CF) is a lethal disease of autosomal recessive character, which affects people of different ethnicities. The disease is characterized by the involvement of systemic exocrine glands and in most patients, the lung disease becomes the predominant pathology. The infection with P. aeruginosa is the leading cause of mortality in patients with CF. The Type III Secretion System (TTSS) of bacteria is expressed during acute disease and injects cytotoxic proteins inside the host cell. There is a great interesting in investigate the antibody response to P. aeruginosa in CF patients in order to diagnose a pulmonary infection or colonization before the culture. Then, preventive antibiotic treatment can be initiated before the installation of chronic lung infection. We investigated the antibody response (IgG + IgA + IgM) against TTSS proteins of P. aeruginosa by Western-blot. The study included 51 patients with CF, from 1.1 to 16.8 years attending the Pediatric Pulmonology Unit of Fernandes Figueira Institute (IFF) FIOCRUZ, Rio de Janeiro, for a period of approximately 2 years. Most patients had or 4 blood samples collected for antibody analyses. Samples were obtained with a mean interval of 6 months. The negative control group consisted of 28 non-CF individuals, from 2 to 17 years, attended at Pedro Ernesto University Hospital - HUPE - UERJ. The TTSS proteins were extracted from strains PAO1 and PAOΔExsA (regulator of TTSS proteins expression) of P. aeruginosa. Positive and negative controls were used in all reactions. For the identification of TTSS proteins in the reaction we used antisera from mice immunized with the recombinant protein PcrV. Twelve (75%) of 16 CF patients considered not infected by P. aeruginosa had their first serology positive for "PopB" and 15 (93.75%) for "ExoS/ExoT . These results indicated that these patients were colonized or infected by P. aeruginosa. About 25% e 35,7% of negative control sera showed a weak reactivity with PopB or ExoS , respectively. The time between the first positive serology and the first isolation of P. aeruginosa in these patients ranged from 18 to 30 months. In conclusion, it is possible to make a serological diagnosis of pulmonary infection by P. aeruginosa before the isolation of the bacterium by culture.