Estudo dos potenciais citotóxico, antineoplásico e mutagênico do extrato etanólico de Hovenia dulcis Thunberg

• Autor(a) principal: Araújo, Luana Brito do Nascimento de
• Data de Publicação: 2017
• Outros Autores: [e-mail]
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/20231
• topic: Biological activity; Plantas medicinais; Citotoxidade; Agentes antineoplásicos; Saccharomyces cerevisiae; Rhamnaceae; Atividade biológica; A549; Vero; CIENCIAS BIOLOGICAS::BOTANICA::BOTANICA APLICADA

Hovenia dulcis Thunberg, is popularly known as Japanese grape, occurring naturally in China, Japan and Korea. The species has many biological activities, highlighting the antineoplastic activity on several cell types. This work aims to evaluate the cytotoxic, antineoplastic and mutagenic potential of the ethanolic extract of Hovenia dulcis leaves. H. dulcis leaves were collected at the Clube dos Médicos in Petropólis (22 ° 30'01.1 "S 43 ° 13'32.8" W), in October 2015. The plant extract used in this work was prepared from leaves immersed in 95% ethanol. Cells of lineage A549 were treated with different concentrations of this extract. After the 24-hour treatment period, cytotoxic activity was analyzed by the methods of WST-1, trypan blue exclusion and clonogenic recovery. The dose that inhibits 50% of cell growth was verified when the cells were exposed to the extract in concentrations of 250 and 275 μg / mL. These concentrations were tested in the normal Vero strain. In this strain, a 10% decrease in cell viability was observed through the trypan blue exclusion tests and 9% in the WST- 1 assay. In the clonogenic recovery assay, there was no statistically significant decrease in the mitogenic capacity of the cells when compared to the control. Experiments were carried out to evaluate the production rate of reactive oxygen species, using flow cytometry using the DCFH-DA reagent, where the extract was shown to be an antioxidant for both tested strains. The yeast Saccharomyces cerevisiae FF18733 (wild) and CD138 (mutant) were also used as a biological model in this study. Both were evaluated for survival fraction, growth, mutagenesis (canavanin resistance test) and petite colonies. And the results showed that the concentration of 150 μg / mL and 100 μg / mL decreased cell viability by 50% in strains FF18733 and CD138 respectively. In the growth test the strains were exposed for 48 hours to the extract of H. dulcis. There was no statistically significant decrease in the FF1873 strain, when compared to the control, whereas the CD138 strain, there was a significant difference in the growth rate. However, in the canavanin resistance test, H. dulcis extract was mutagenic for both strains. In the experiments to evaluate the loss of mitochondrial function, it was observed that in both strains tested they presented an increase in the rate of petite colonies. Comparing the results observed in the upper and lower eukaryotes, we observed that in both cases there was an alteration in the oxidative pathways that affect the mitochondrial metabolism, suggesting that this organelle is the most affected by the products of the extract of H. dulcis, being necessary a study analyzing the pathway cell death, and about the chemical composition of the extract.