A composição corporal, resistência à leptina e função tireóidea são programadas em ratos cujas mães foram tratadas com leptina no início da lactação
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Biblioteca Digital de Teses e Dissertações da UERJ |
Texto Completo: | http://www.bdtd.uerj.br/handle/1/12774 |
Resumo: | Pup´s leptin injection on the first 10 days of lactation programmes for higher food intake (FI), body mass (BM), lean mass, thyroid function, hyperleptinemia and resistance to its action and lower leptin receptor (ObRb) expression on 150 days-old rats. When mothers were leptin-treated on the last 3 days of lactation, it seems to reproduce partially this programming effect, except for higher visceral fat mass (VFM). So, we evaluated the offspring metabolic phenotype when the mothers were treated with leptin in the first 10 days of lactation. On birth, the lactating Wistar rats were divided into: Leptin (LEP) treated with recombinant leptin (8mg/100g, PC, sc) for the first 10 days of lactation and Control (C) saline-treated in the same conditions. The mothers´ BM and FI were monitored daily and they were milked at the 21st day of lactation. LEP mothers had lower BM during lactation (~6%, p <0.05) and normal FI. The mothers sacrifice occurred to the end of lactation. The leptin concentration of LEP mothers was normal in serum and milk, while T3 was normal in serum and higher in milk (+30%, p <0.05). The offspring BM and FI was monitored daily and accompanied by 4 on 4 days after weaning until 180 days of age. The LEP offspring had lower BM during lactation (~5%, p<0.05) and from the day 69 onward higher BM (+10%, p<0.05), while the FI was higher (+17%, p<0.05) at day 145 onward. The leptin resistance test was performed at 30 and 180 days. Both group was subdivided into: CLEP and LEPLEP treated with leptin (0.5 mg / kg / ip PC); CSAL and LEPSAL treated with saline. The animals were sacrificed at 21, 30 and 180 days. We collected the VFM, carcass, blood (glycemia, leptin, total T3 and T4, TSH, insulin) and liver (GPDm activity). LEP offspring had higher T3 at 21 days, but not significant (p = 0.06) and when they were 30 days-old they already present leptin resistance and lower serum T3 (-20%,p<0.05). At 180 days they had higher VFM (+57%, p<0.05), total body fat (+40%, p<0.05), leptin resistance, hyperleptinemia (+1,35x, p<0.05) with normal 125I thyroid uptake, serum T4 and TSH and lower GPDm activity at 30 and 180 day (-42% and -57%,p< 0.05 respectively). So, the mother's hyperleptinaemia in the beginning of lactation programs as early as the 30 days-old offspring: leptin resistance and hypothyroidism, probably by a higher leptin transfer through the milk. We suggested that the levels of leptin in early lactation are determinants of the physiological regulation of energy balance in adulthood. We suggest that the programming effects are dependent on the way that leptin reaches the offspring. The present study seems more physiological and reproduces almost completely the programming effect in the adulthood when the mothers were leptin-injected at the end of lactation and, partially reproduces the effects when leptin was directally injected in the pups. |
id |
UERJ_d92908e9b7e849d9b846fdb28ebca50f |
---|---|
oai_identifier_str |
oai:www.bdtd.uerj.br:1/12774 |
network_acronym_str |
UERJ |
network_name_str |
Biblioteca Digital de Teses e Dissertações da UERJ |
repository_id_str |
2903 |
spelling |
Passos, Magna Cottini Fonsecahttp://lattes.cnpq.br/5002729708699349Silva, Patrícia Cristina Lisbôa dahttp://lattes.cnpq.br/6704904748735082Moura, Carmen Cabanelas Pazos dehttp://lattes.cnpq.br/0104272542847732Costa, Célia Lopes dahttp://lattes.cnpq.br/9103348473357276Moura, Egberto Gaspar dehttp://lattes.cnpq.br/9398848717949756http://lattes.cnpq.br/9740593519074623Toste, Fernanda Pereira2021-01-06T20:58:22Z2009-12-012008-03-27TOSTE, Fernanda Pereira. A composição corporal, resistência à leptina e função tireóidea são programadas em ratos cujas mães foram tratadas com leptina no início da lactação. 2008. 102 f. Dissertação (Mestrado em Fisiopatologia Clínica e Experimental) - Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2008.http://www.bdtd.uerj.br/handle/1/12774Pup´s leptin injection on the first 10 days of lactation programmes for higher food intake (FI), body mass (BM), lean mass, thyroid function, hyperleptinemia and resistance to its action and lower leptin receptor (ObRb) expression on 150 days-old rats. When mothers were leptin-treated on the last 3 days of lactation, it seems to reproduce partially this programming effect, except for higher visceral fat mass (VFM). So, we evaluated the offspring metabolic phenotype when the mothers were treated with leptin in the first 10 days of lactation. On birth, the lactating Wistar rats were divided into: Leptin (LEP) treated with recombinant leptin (8mg/100g, PC, sc) for the first 10 days of lactation and Control (C) saline-treated in the same conditions. The mothers´ BM and FI were monitored daily and they were milked at the 21st day of lactation. LEP mothers had lower BM during lactation (~6%, p <0.05) and normal FI. The mothers sacrifice occurred to the end of lactation. The leptin concentration of LEP mothers was normal in serum and milk, while T3 was normal in serum and higher in milk (+30%, p <0.05). The offspring BM and FI was monitored daily and accompanied by 4 on 4 days after weaning until 180 days of age. The LEP offspring had lower BM during lactation (~5%, p<0.05) and from the day 69 onward higher BM (+10%, p<0.05), while the FI was higher (+17%, p<0.05) at day 145 onward. The leptin resistance test was performed at 30 and 180 days. Both group was subdivided into: CLEP and LEPLEP treated with leptin (0.5 mg / kg / ip PC); CSAL and LEPSAL treated with saline. The animals were sacrificed at 21, 30 and 180 days. We collected the VFM, carcass, blood (glycemia, leptin, total T3 and T4, TSH, insulin) and liver (GPDm activity). LEP offspring had higher T3 at 21 days, but not significant (p = 0.06) and when they were 30 days-old they already present leptin resistance and lower serum T3 (-20%,p<0.05). At 180 days they had higher VFM (+57%, p<0.05), total body fat (+40%, p<0.05), leptin resistance, hyperleptinemia (+1,35x, p<0.05) with normal 125I thyroid uptake, serum T4 and TSH and lower GPDm activity at 30 and 180 day (-42% and -57%,p< 0.05 respectively). So, the mother's hyperleptinaemia in the beginning of lactation programs as early as the 30 days-old offspring: leptin resistance and hypothyroidism, probably by a higher leptin transfer through the milk. We suggested that the levels of leptin in early lactation are determinants of the physiological regulation of energy balance in adulthood. We suggest that the programming effects are dependent on the way that leptin reaches the offspring. The present study seems more physiological and reproduces almost completely the programming effect in the adulthood when the mothers were leptin-injected at the end of lactation and, partially reproduces the effects when leptin was directally injected in the pups.A morbidade e mortalidade por doenças cardiovasculares demonstram tendência geral de declínio, mas, em países em desenvolvimento como o Brasil a ocorrência destes eventos é crescente. A obesidade e principalmente a localização intra-abdominal de gordura, relaciona-se com a ocorrência de doença crônica e diferentes tipos de dietas tem sido testados na busca pela efetiva redução da adiposidade. Fatores biológicos como a resistência à insulina pode interferir na resposta obtida com intervenções nutricionais. O objetivo deste estudo foi avaliar se a perda de peso e as mudanças ocorridas na composição corporal de mulheres saudáveis, eutróficas ou com sobrepeso, submetidas a um programa de prevenção de ganho de peso foram influenciadas pela resistência à insulina no inicio da intervenção. Trata-se de um estudo observacional prospectivo. 203 mulheres foram alocadas randomicamente para dieta de baixo e alto índice glicêmico. Destas, 185, foram avaliadas quanto a presença de resistência a insulina na linha de base, 34,6% foram classificadas como resistentes a insulina segundo o índice HOMA-IR, no ponto de corte 2,71. As medidas antropométricas de localização de gordura, circunferência da cintura (CC) e relação cintura quadril (RCQ) associaram-se com a resistência a insulina do inicio do estudo, sendo a RCQ a mais fortemente associada (razão de prevalência: 2,28; p=0,0005, enquanto que para CC o valor foi 1,53; p=0,04). A análise da modificação do peso e das medidas antropométricas de composição corporal ao longo dos 6 meses de acompanhamento não demonstrou diferença estatisticamente significante entre os grupos com e sem resistência a insulina. Em conclusão, embora a resistência à insulina tenha se correlacionado com a localização de gordura avaliada principalmente pela relação cintura quadril no inicio do estudo, ela não foi capaz de explicar mudanças na composição corporal e de peso em resposta a uma intervenção nutricional.Submitted by Boris Flegr (boris@uerj.br) on 2021-01-06T20:58:22Z No. of bitstreams: 1 Fernanda PereiraToste Completa.pdf: 459831 bytes, checksum: a71eb539dfd421a66d25391e6e60d7f6 (MD5)Made available in DSpace on 2021-01-06T20:58:22Z (GMT). No. of bitstreams: 1 Fernanda PereiraToste Completa.pdf: 459831 bytes, checksum: a71eb539dfd421a66d25391e6e60d7f6 (MD5) Previous issue date: 2008-03-27Coordenação de Aperfeiçoamento de Pessoal de Nível Superiorapplication/pdfporUniversidade do Estado do Rio de JaneiroPrograma de Pós-Graduação em Fisiopatologia Clínica e ExperimentalUERJBRCentro Biomédico::Faculdade de Ciências MédicasProgrammingLeptinlLptin resistanceThyroid functionBody compositionResistência à insulinaHOMASensibilidade à insulinaPerda de pesoCircunferência da cinturaRelação cintura quadrilComposição corporalBaixo índice glicêmicoCNPQ::CIENCIAS BIOLOGICAS::FISIOLOGIA::FISIOLOGIA DE ORGAOS E SISTEMAS::FISIOLOGIA ENDOCRINAA composição corporal, resistência à leptina e função tireóidea são programadas em ratos cujas mães foram tratadas com leptina no início da lactaçãoThe body composition leptin resistance and thyroid function are programmed in rats whose mothers were treated with leptin in early lactationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisinfo:eu-repo/semantics/openAccessreponame:Biblioteca Digital de Teses e Dissertações da UERJinstname:Universidade do Estado do Rio de Janeiro (UERJ)instacron:UERJORIGINALFernanda PereiraToste Completa.pdfapplication/pdf459831http://www.bdtd.uerj.br/bitstream/1/12774/1/Fernanda+PereiraToste+Completa.pdfa71eb539dfd421a66d25391e6e60d7f6MD511/127742024-02-26 16:36:36.567oai:www.bdtd.uerj.br:1/12774Biblioteca Digital de Teses e Dissertaçõeshttp://www.bdtd.uerj.br/PUBhttps://www.bdtd.uerj.br:8443/oai/requestbdtd.suporte@uerj.bropendoar:29032024-02-26T19:36:36Biblioteca Digital de Teses e Dissertações da UERJ - Universidade do Estado do Rio de Janeiro (UERJ)false |
dc.title.por.fl_str_mv |
A composição corporal, resistência à leptina e função tireóidea são programadas em ratos cujas mães foram tratadas com leptina no início da lactação |
dc.title.alternative.eng.fl_str_mv |
The body composition leptin resistance and thyroid function are programmed in rats whose mothers were treated with leptin in early lactation |
title |
A composição corporal, resistência à leptina e função tireóidea são programadas em ratos cujas mães foram tratadas com leptina no início da lactação |
spellingShingle |
A composição corporal, resistência à leptina e função tireóidea são programadas em ratos cujas mães foram tratadas com leptina no início da lactação Toste, Fernanda Pereira Programming Leptin lLptin resistance Thyroid function Body composition Resistência à insulina HOMA Sensibilidade à insulina Perda de peso Circunferência da cintura Relação cintura quadril Composição corporal Baixo índice glicêmico CNPQ::CIENCIAS BIOLOGICAS::FISIOLOGIA::FISIOLOGIA DE ORGAOS E SISTEMAS::FISIOLOGIA ENDOCRINA |
title_short |
A composição corporal, resistência à leptina e função tireóidea são programadas em ratos cujas mães foram tratadas com leptina no início da lactação |
title_full |
A composição corporal, resistência à leptina e função tireóidea são programadas em ratos cujas mães foram tratadas com leptina no início da lactação |
title_fullStr |
A composição corporal, resistência à leptina e função tireóidea são programadas em ratos cujas mães foram tratadas com leptina no início da lactação |
title_full_unstemmed |
A composição corporal, resistência à leptina e função tireóidea são programadas em ratos cujas mães foram tratadas com leptina no início da lactação |
title_sort |
A composição corporal, resistência à leptina e função tireóidea são programadas em ratos cujas mães foram tratadas com leptina no início da lactação |
author |
Toste, Fernanda Pereira |
author_facet |
Toste, Fernanda Pereira |
author_role |
author |
dc.contributor.advisor1.fl_str_mv |
Passos, Magna Cottini Fonseca |
dc.contributor.advisor1Lattes.fl_str_mv |
http://lattes.cnpq.br/5002729708699349 |
dc.contributor.advisor-co1.fl_str_mv |
Silva, Patrícia Cristina Lisbôa da |
dc.contributor.advisor-co1Lattes.fl_str_mv |
http://lattes.cnpq.br/6704904748735082 |
dc.contributor.referee1.fl_str_mv |
Moura, Carmen Cabanelas Pazos de |
dc.contributor.referee1Lattes.fl_str_mv |
http://lattes.cnpq.br/0104272542847732 |
dc.contributor.referee2.fl_str_mv |
Costa, Célia Lopes da |
dc.contributor.referee2Lattes.fl_str_mv |
http://lattes.cnpq.br/9103348473357276 |
dc.contributor.referee3.fl_str_mv |
Moura, Egberto Gaspar de |
dc.contributor.referee3Lattes.fl_str_mv |
http://lattes.cnpq.br/9398848717949756 |
dc.contributor.authorLattes.fl_str_mv |
http://lattes.cnpq.br/9740593519074623 |
dc.contributor.author.fl_str_mv |
Toste, Fernanda Pereira |
contributor_str_mv |
Passos, Magna Cottini Fonseca Silva, Patrícia Cristina Lisbôa da Moura, Carmen Cabanelas Pazos de Costa, Célia Lopes da Moura, Egberto Gaspar de |
dc.subject.eng.fl_str_mv |
Programming Leptin lLptin resistance Thyroid function Body composition |
topic |
Programming Leptin lLptin resistance Thyroid function Body composition Resistência à insulina HOMA Sensibilidade à insulina Perda de peso Circunferência da cintura Relação cintura quadril Composição corporal Baixo índice glicêmico CNPQ::CIENCIAS BIOLOGICAS::FISIOLOGIA::FISIOLOGIA DE ORGAOS E SISTEMAS::FISIOLOGIA ENDOCRINA |
dc.subject.por.fl_str_mv |
Resistência à insulina HOMA Sensibilidade à insulina Perda de peso Circunferência da cintura Relação cintura quadril Composição corporal Baixo índice glicêmico |
dc.subject.cnpq.fl_str_mv |
CNPQ::CIENCIAS BIOLOGICAS::FISIOLOGIA::FISIOLOGIA DE ORGAOS E SISTEMAS::FISIOLOGIA ENDOCRINA |
description |
Pup´s leptin injection on the first 10 days of lactation programmes for higher food intake (FI), body mass (BM), lean mass, thyroid function, hyperleptinemia and resistance to its action and lower leptin receptor (ObRb) expression on 150 days-old rats. When mothers were leptin-treated on the last 3 days of lactation, it seems to reproduce partially this programming effect, except for higher visceral fat mass (VFM). So, we evaluated the offspring metabolic phenotype when the mothers were treated with leptin in the first 10 days of lactation. On birth, the lactating Wistar rats were divided into: Leptin (LEP) treated with recombinant leptin (8mg/100g, PC, sc) for the first 10 days of lactation and Control (C) saline-treated in the same conditions. The mothers´ BM and FI were monitored daily and they were milked at the 21st day of lactation. LEP mothers had lower BM during lactation (~6%, p <0.05) and normal FI. The mothers sacrifice occurred to the end of lactation. The leptin concentration of LEP mothers was normal in serum and milk, while T3 was normal in serum and higher in milk (+30%, p <0.05). The offspring BM and FI was monitored daily and accompanied by 4 on 4 days after weaning until 180 days of age. The LEP offspring had lower BM during lactation (~5%, p<0.05) and from the day 69 onward higher BM (+10%, p<0.05), while the FI was higher (+17%, p<0.05) at day 145 onward. The leptin resistance test was performed at 30 and 180 days. Both group was subdivided into: CLEP and LEPLEP treated with leptin (0.5 mg / kg / ip PC); CSAL and LEPSAL treated with saline. The animals were sacrificed at 21, 30 and 180 days. We collected the VFM, carcass, blood (glycemia, leptin, total T3 and T4, TSH, insulin) and liver (GPDm activity). LEP offspring had higher T3 at 21 days, but not significant (p = 0.06) and when they were 30 days-old they already present leptin resistance and lower serum T3 (-20%,p<0.05). At 180 days they had higher VFM (+57%, p<0.05), total body fat (+40%, p<0.05), leptin resistance, hyperleptinemia (+1,35x, p<0.05) with normal 125I thyroid uptake, serum T4 and TSH and lower GPDm activity at 30 and 180 day (-42% and -57%,p< 0.05 respectively). So, the mother's hyperleptinaemia in the beginning of lactation programs as early as the 30 days-old offspring: leptin resistance and hypothyroidism, probably by a higher leptin transfer through the milk. We suggested that the levels of leptin in early lactation are determinants of the physiological regulation of energy balance in adulthood. We suggest that the programming effects are dependent on the way that leptin reaches the offspring. The present study seems more physiological and reproduces almost completely the programming effect in the adulthood when the mothers were leptin-injected at the end of lactation and, partially reproduces the effects when leptin was directally injected in the pups. |
publishDate |
2008 |
dc.date.issued.fl_str_mv |
2008-03-27 |
dc.date.available.fl_str_mv |
2009-12-01 |
dc.date.accessioned.fl_str_mv |
2021-01-06T20:58:22Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
TOSTE, Fernanda Pereira. A composição corporal, resistência à leptina e função tireóidea são programadas em ratos cujas mães foram tratadas com leptina no início da lactação. 2008. 102 f. Dissertação (Mestrado em Fisiopatologia Clínica e Experimental) - Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2008. |
dc.identifier.uri.fl_str_mv |
http://www.bdtd.uerj.br/handle/1/12774 |
identifier_str_mv |
TOSTE, Fernanda Pereira. A composição corporal, resistência à leptina e função tireóidea são programadas em ratos cujas mães foram tratadas com leptina no início da lactação. 2008. 102 f. Dissertação (Mestrado em Fisiopatologia Clínica e Experimental) - Universidade do Estado do Rio de Janeiro, Rio de Janeiro, 2008. |
url |
http://www.bdtd.uerj.br/handle/1/12774 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade do Estado do Rio de Janeiro |
dc.publisher.program.fl_str_mv |
Programa de Pós-Graduação em Fisiopatologia Clínica e Experimental |
dc.publisher.initials.fl_str_mv |
UERJ |
dc.publisher.country.fl_str_mv |
BR |
dc.publisher.department.fl_str_mv |
Centro Biomédico::Faculdade de Ciências Médicas |
publisher.none.fl_str_mv |
Universidade do Estado do Rio de Janeiro |
dc.source.none.fl_str_mv |
reponame:Biblioteca Digital de Teses e Dissertações da UERJ instname:Universidade do Estado do Rio de Janeiro (UERJ) instacron:UERJ |
instname_str |
Universidade do Estado do Rio de Janeiro (UERJ) |
instacron_str |
UERJ |
institution |
UERJ |
reponame_str |
Biblioteca Digital de Teses e Dissertações da UERJ |
collection |
Biblioteca Digital de Teses e Dissertações da UERJ |
bitstream.url.fl_str_mv |
http://www.bdtd.uerj.br/bitstream/1/12774/1/Fernanda+PereiraToste+Completa.pdf |
bitstream.checksum.fl_str_mv |
a71eb539dfd421a66d25391e6e60d7f6 |
bitstream.checksumAlgorithm.fl_str_mv |
MD5 |
repository.name.fl_str_mv |
Biblioteca Digital de Teses e Dissertações da UERJ - Universidade do Estado do Rio de Janeiro (UERJ) |
repository.mail.fl_str_mv |
bdtd.suporte@uerj.br |
_version_ |
1792352317007200256 |