Efeitos da castração e da reposição hormonal com undecanoato de testosterona na bexiga urinária de ratos: análise estrutural, ultra-estrutural e bioquímica

• Autor(a) principal: Gallo, Carla Braga Mano
• Data de Publicação: 2009
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/12331
• topic: Bladder; Rat; Testosterone; Androgen deficiency of the aging male; Hormonal replacement; Histology; Bexiga; Rato; Testosterona; Deficiência androgênica do envelhecimento masculino; Reposição hormonal; Histologia; Bexiga-histologia; Terapia de reposição hormonal; CNPQ::CIENCIAS DA SAUDE::MEDICINA

Recent evidences in animals and humans have suggested that adequate levels of testosterone are necessary to adequate functions of diverse organs of the urogenital system, including the urinary bladder. Studies on the effects of testosterone in the bladder wall structure are rare. Therefore, the objective of the present study is to evaluate, through qualitative and quantitative methods, the structural alterations in the bladder wall of rats submitted to surgical castration as well as the role of hormonal replacement in reversing the possible structural alterations. We used 24 male Sprague-Dawley rats that were approximately 12 weeks of age. The animals were divided into 3 groups composed of 8 animals each and treated as follows. Group C = group that underwent orchiectomy and were sacrificed after 2 months, Group S = sham group sacrificed after 2 months, and Group T = group that underwent orchiectomy, and after 1 month underwent testosterone replacement with a subcutaneous single dose of testosterone undecanoate at 100 mg/kg (T) and after 1 month of hormonal replacement, the animals were sacrificed. We performed a qualitative and quantitative analysis of collagen (by using histochemistry, histomorphometry, biochemistry, and scanning electron microscopy), and a histomorphometric analysis of smooth muscle and elastic system fibers in bladder wall of controls and rats submitted to orchiectomy alone and with hormonal replacement. The histomorphometric analysis on the epithelial height did not show differences among the groups. There was no statistically significant difference in the quantitative analysis for collagen, both by histomorphometry and biochemistry. Nevertheless, the qualitative analysis showed differences in collagen in the castrated group, when compared to controls and to rats with hormonal replacement. There was a significant decrease in the absolute values of elastic system fibers in the castrated group. On the other hand, the smooth muscle presented a significant increase in muscular mass by density of area in castrated rats; nevertheless, the counting of muscle cells nuclei did not present variation among the groups, demonstrating that this increase was due to cellular hypertrophy rather than by an increase in cells number. Interestingly, the hormonal replacement with testosterone was able to reverse all alterations observed. The results suggest that hormonal replacement, even when instituted at a late stage, is effective in reversing the bladder wall alterations produced by secondary hypogonadism.