Avaliação do comportamento físico e microbiológico da incorporação de diacetato de clorexidina a resinas acrílicas a base de PMMA

• Autor(a) principal: Peroni, Luciana Vieira
• Data de Publicação: 2014
• Tipo de documento: Dissertação
• Idioma: por
• Título da fonte: Biblioteca Digital de Teses e Dissertações da UERJ
• Texto Completo: http://www.bdtd.uerj.br/handle/1/14171
• topic: Acrylic resins; Chlorhexidine diacetate; Candida albicans; Antifungal activity; Release of chlorhexidine; Physical properties; Resinas acrílicas; Diacetato de clorexidina; Candida albicans; Atividade antifúngica; Liberação de clorexidina; Propriedades físicas; CNPQ::CIENCIAS DA SAUDE::ODONTOLOGIA

This work aimed to evaluate physical and microbiological properties of PMMA acrylic resins after incorporating chlorhexidine diacetate salt (CDA) to them . For that, specimens ( CDPs ) were made with resins VIPI COR ® and Duralay ® , with and without incorporation of 0.5 % , 1.0 % and 2.0 % of CDA , totaling 8 groups . The liquid chromatography was used to measure the release of CDA by acrylic resins , and also to measure leaching of residual monomers . For that, CDPs were stored individually in cell culture plates to 24 well containing 1 ml of sterile distilled water in each well. Storage time after 2 hours , 7 days, 14 days, 21 days and 28 days at 37 ° C , the solution was removed and release of residual monomers or chlorhexidine was evaluated by using ultraviolet spectrometry combined with HPLC. The antifungal activity to C. albicans was evaluated using the agar diffusion method , in which the CDPs were placed on BHI plates inoculated with C. albicans with measuring the zone of inhibition after 48 hours incubation at 37 ° C. The analysis of the resins´ conversion degree is given by infrared spectroscopy Fourier transform - FTIR using an uncured resin sample in each group and 4 scans performed absorbance . For the measurement of resins containing CDA ´s water sorption, 10 specimens were prepared for each group , which were placed in support within desiccator ± 37 º C to remove intrinsic ( m1 ) and then immersed moisture in 100 ml deionized water for 7 days at ± 37 ° C , and the water was changed daily. After this interval , the specimens were dried to obtain the new resin´s weight ( m2 ) . The masses obtained were included in the mathematical formula for the degree of sorption. After obtaining results, when compared the inhibition halo between the tested groups and the same brand , only the analyzes between group CDA2 % x CDA 1 % group and from CDA 1 % x CDA0.5 %,showed no significant differences . About the release of CDA , analysis of variance showed that two of the three factors assessed ( drug concentration and storage time ) significantly change the rate of release of chlorhexidine ( p < 0.0001 ) , however the material mark seemed not significantly influence the drug release . About the conversion degree , the values were not significant and were minor only in groups with CDA 2 % . For both water sorption increased with the incorporation of salt grew and there was significant increase in concentrations of 1.0 % and 2.0 % . We conclude that the incorporation of chlorhexidine to the PMMA based resin : is able to inhibit the growth of C. Albicans ; did not alter the degree of conversion of the resins tested , does not alter the release of residual monomers , and changes the water sorption of the base PMMA acrylic resins when CDA concentrations are incorporated.