Dietas padrão utilizadas em experimentação animal : uma análise comparativa.
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Tipo de documento: | Dissertação |
Idioma: | por |
Título da fonte: | Repositório Institucional da Universidade Federal de Alagoas (UFAL) |
Texto Completo: | http://repositorio.ufal.br/handle/riufal/637 |
Resumo: | Many diets of different compositions are available for use in animal experiments and have been used as standard, but they may induce adverse metabolic effects, compromising the comparison between the results of several studies. The literature records many reports of changes related to the use of these diets, however it lacks studies that compare metabolic effects of consumption of the different standard diets in animal experiments. Accordingly, the objective of this dissertation was to evaluate the metabolic effects of the consumption of diets considered standard widely used in animal research, being presented in the form of two articles: a review of the literature that gathers evidence yet little discussed by the scientific community on the feeding of laboratory animals; the second article refers to an experimental study with rats newly weaned, who received two types of diets: a commercial cereal-based, Nuvilab®, and another purified proposal by the American Institute of Nutrition, the AIN-93. Under the experimental conditions established, coefficients of protein and feeding efficiency presented significantly higher in group AIN-93 than in group Nuvilab®. The AIN-93 showed significantly higher lipid and protein digestibility than Nuvilab®. The different diets did not cause weight difference evolution of animals and histological analysis to the optical microscope of the kidneys, heart, spleen, stomach and small intestine showed no changes in the structures of these bodies, despite the different treatments. Animals fed the AIN-93 diet, regardless of age, had hepatic steatosis in frequency significantly higher than the animals that received the commercial Nuvilab®. The different diets did not cause influence on the absolute and relative weights of organs of animals, except for the absolute weight of the liver among younger animals and relative weight of the intestine among older animals. There was no influence of different diets on biochemical parameters evaluated, and the differences detected possibly resulting from the interaction between age and length of exposure of animals to diets. The markers of damage of kidney and liver function were similar and serum creatinine varied according to age. It was shown that both diets, AIN-93 and Nuvilab ®, are able to promote the growth of rats for a period of study considered subchronic. However, the occurrence of hepatic steatosis in animals fed the AIN-93 diet in pellets, reinforces the importance of tracking the standard protocols of experimentation and is indicative of nutritional inadequacies by imposing the need for further investigations to clarify which components or characteristics of this diet, widely used in animal experiments, may have contributed to this result. For instance, it is suggested that diets in the form of flour are used preferably those pellets, particularly on protocols to investigate metabolic effects. |
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Dietas padrão utilizadas em experimentação animal : uma análise comparativa.Standard diets used in animal experimentation : a comparative analysis.Wistar ratAIN-93-GAIN-93MHepatic steatosisExperimental dietsAdvanced glycation endproductsDieta experimental RatosEsteatose hepáticaFígadoCNPQ::CIENCIAS DA SAUDE::NUTRICAOMany diets of different compositions are available for use in animal experiments and have been used as standard, but they may induce adverse metabolic effects, compromising the comparison between the results of several studies. The literature records many reports of changes related to the use of these diets, however it lacks studies that compare metabolic effects of consumption of the different standard diets in animal experiments. Accordingly, the objective of this dissertation was to evaluate the metabolic effects of the consumption of diets considered standard widely used in animal research, being presented in the form of two articles: a review of the literature that gathers evidence yet little discussed by the scientific community on the feeding of laboratory animals; the second article refers to an experimental study with rats newly weaned, who received two types of diets: a commercial cereal-based, Nuvilab®, and another purified proposal by the American Institute of Nutrition, the AIN-93. Under the experimental conditions established, coefficients of protein and feeding efficiency presented significantly higher in group AIN-93 than in group Nuvilab®. The AIN-93 showed significantly higher lipid and protein digestibility than Nuvilab®. The different diets did not cause weight difference evolution of animals and histological analysis to the optical microscope of the kidneys, heart, spleen, stomach and small intestine showed no changes in the structures of these bodies, despite the different treatments. Animals fed the AIN-93 diet, regardless of age, had hepatic steatosis in frequency significantly higher than the animals that received the commercial Nuvilab®. The different diets did not cause influence on the absolute and relative weights of organs of animals, except for the absolute weight of the liver among younger animals and relative weight of the intestine among older animals. There was no influence of different diets on biochemical parameters evaluated, and the differences detected possibly resulting from the interaction between age and length of exposure of animals to diets. The markers of damage of kidney and liver function were similar and serum creatinine varied according to age. It was shown that both diets, AIN-93 and Nuvilab ®, are able to promote the growth of rats for a period of study considered subchronic. However, the occurrence of hepatic steatosis in animals fed the AIN-93 diet in pellets, reinforces the importance of tracking the standard protocols of experimentation and is indicative of nutritional inadequacies by imposing the need for further investigations to clarify which components or characteristics of this diet, widely used in animal experiments, may have contributed to this result. For instance, it is suggested that diets in the form of flour are used preferably those pellets, particularly on protocols to investigate metabolic effects.Fundação de Amparo a Pesquisa do Estado de AlagoasVárias dietas de diferentes composições estão disponíveis para o uso em experimentação animal e têm sido utilizadas como padrão, mas podem induzir efeitos metabólicos distintos, comprometendo a comparação entre os resultados dos diversos estudos. A literatura registra inúmeros relatos de alterações relacionadas ao uso dessas dietas, porém, há carência de estudos que comparem os efeitos metabólicos do consumo das diferentes dietas padrão utilizadas em experimentos animais. Assim sendo, o objetivo da presente dissertação foi avaliar as repercussões metabólicas do consumo de dietas consideradas padrão, amplamente utilizadas na pesquisa animal, sendo apresentada na forma de dois artigos: uma revisão da literatura, que reúne evidências ainda pouco debatidas pela comunidade científica relativas à alimentação de animais de laboratório, e um segundo artigo, que se refere a um estudo experimental com ratos Wistar recémdesmamados, que receberam dois tipos de dieta: uma comercial à base de cereais, Nuvilab®, e outra purificada proposta pelo American Institute of Nutrition, a AIN-93. Nas condições experimentais estabelecidas, coeficientes de eficiências protéica e alimentar apresentaram-se significativamente maiores no grupo AIN-93 que no grupo Nuvilab®. A AIN-93 apresentou digestibilidades lipídica e protéica significativamente maiores que a Nuvilab®. As diferentes dietas não causaram diferença na evolução ponderal dos animais e a análise histológica ao microscópio óptico dos rins, coração, baço, estômago e intestinos não evidenciou alterações nas estruturas desses órgãos, apesar dos diferentes tratamentos. Os animais alimentados com a dieta AIN-93, independente da idade, apresentaram esteatose hepática em uma frequência significativamente maior que os animais que receberam a comercial Nuvilab®. As diferentes dietas não exerceram influência sobre os pesos absoluto e relativo dos órgãos dos animais, com exceção do peso absoluto do fígado, entre os animais mais jovens, e do peso relativo do intestino, entre os animais mais velhos. Não se observou influência das diferentes dietas sobre os parâmetros bioquímicos avaliados, sendo as diferenças detectadas possivelmente resultantes da interação entre a idade e o tempo de exposição dos animais às dietas. Os marcadores de lesão e função hepática e renal foram similares e a creatinina sérica variou em função da idade. Demonstrou-se que ambas as dietas, AIN-93 e Nuvilab®, são capazes de promover o crescimento de ratos Wistar por um período de estudo considerado subcrônico. Porém, a ocorrência de esteatose hepática nos animais alimentados com a dieta AIN-93 peletizada, reforça a importância de monitoramento de protocolos padrão de experimentação e é indicativa de inadequações nutricionais, impondo a necessidade de investigações adicionais para esclarecer que componentes ou características dessa dieta, amplamente utilizada em experimentação animal, podem ter contribuído para tal resultado. Por hora, sugere-se que a dieta AIN-93 seja oferecida preferencialmente na forma de farinha, particularmente em protocolos que investiguem efeitos metabólicos.Universidade Federal de AlagoasBRNutriçãoPrograma de Pós-Graduação em NutriçãoUFALOliveira, Suzana Lima deOLIVEIRA, S. L.Ataíde, Terezinha da RochaATAÍDE, Terezinha da RochaRibeiro, êurica Adélia NogueiraRIBEIRO, Ê. A. N.Trindade Filho, Euclides MauricioTRINDADE FILHO, E. M.Barbosa, Junia Helena Porto2015-08-25T18:37:14Z2010-01-222015-08-25T18:37:14Z2008-11-12info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/masterThesisapplication/pdfapplication/pdfBARBOSA, Junia Helena Porto. Standard diets used in animal experimentation : a comparative analysis.. 2008. 78 f. Dissertação (Mestrado em Nutrição) - Universidade Federal de Alagoas, Maceió, 2008.http://repositorio.ufal.br/handle/riufal/637porinfo:eu-repo/semantics/embargoedAccessreponame:Repositório Institucional da Universidade Federal de Alagoas (UFAL)instname:Universidade Federal de Alagoas (UFAL)instacron:UFAL2018-11-27T22:40:34Zoai:www.repositorio.ufal.br:riufal/637Repositório InstitucionalPUBhttp://www.repositorio.ufal.br/oai/requestri@sibi.ufal.bropendoar:2018-11-27T22:40:34Repositório Institucional da Universidade Federal de Alagoas (UFAL) - Universidade Federal de Alagoas (UFAL)false |
dc.title.none.fl_str_mv |
Dietas padrão utilizadas em experimentação animal : uma análise comparativa. Standard diets used in animal experimentation : a comparative analysis. |
title |
Dietas padrão utilizadas em experimentação animal : uma análise comparativa. |
spellingShingle |
Dietas padrão utilizadas em experimentação animal : uma análise comparativa. Barbosa, Junia Helena Porto Wistar rat AIN-93-G AIN-93M Hepatic steatosis Experimental diets Advanced glycation endproducts Dieta experimental Ratos Esteatose hepática Fígado CNPQ::CIENCIAS DA SAUDE::NUTRICAO |
title_short |
Dietas padrão utilizadas em experimentação animal : uma análise comparativa. |
title_full |
Dietas padrão utilizadas em experimentação animal : uma análise comparativa. |
title_fullStr |
Dietas padrão utilizadas em experimentação animal : uma análise comparativa. |
title_full_unstemmed |
Dietas padrão utilizadas em experimentação animal : uma análise comparativa. |
title_sort |
Dietas padrão utilizadas em experimentação animal : uma análise comparativa. |
author |
Barbosa, Junia Helena Porto |
author_facet |
Barbosa, Junia Helena Porto |
author_role |
author |
dc.contributor.none.fl_str_mv |
Oliveira, Suzana Lima de OLIVEIRA, S. L. Ataíde, Terezinha da Rocha ATAÍDE, Terezinha da Rocha Ribeiro, êurica Adélia Nogueira RIBEIRO, Ê. A. N. Trindade Filho, Euclides Mauricio TRINDADE FILHO, E. M. |
dc.contributor.author.fl_str_mv |
Barbosa, Junia Helena Porto |
dc.subject.por.fl_str_mv |
Wistar rat AIN-93-G AIN-93M Hepatic steatosis Experimental diets Advanced glycation endproducts Dieta experimental Ratos Esteatose hepática Fígado CNPQ::CIENCIAS DA SAUDE::NUTRICAO |
topic |
Wistar rat AIN-93-G AIN-93M Hepatic steatosis Experimental diets Advanced glycation endproducts Dieta experimental Ratos Esteatose hepática Fígado CNPQ::CIENCIAS DA SAUDE::NUTRICAO |
description |
Many diets of different compositions are available for use in animal experiments and have been used as standard, but they may induce adverse metabolic effects, compromising the comparison between the results of several studies. The literature records many reports of changes related to the use of these diets, however it lacks studies that compare metabolic effects of consumption of the different standard diets in animal experiments. Accordingly, the objective of this dissertation was to evaluate the metabolic effects of the consumption of diets considered standard widely used in animal research, being presented in the form of two articles: a review of the literature that gathers evidence yet little discussed by the scientific community on the feeding of laboratory animals; the second article refers to an experimental study with rats newly weaned, who received two types of diets: a commercial cereal-based, Nuvilab®, and another purified proposal by the American Institute of Nutrition, the AIN-93. Under the experimental conditions established, coefficients of protein and feeding efficiency presented significantly higher in group AIN-93 than in group Nuvilab®. The AIN-93 showed significantly higher lipid and protein digestibility than Nuvilab®. The different diets did not cause weight difference evolution of animals and histological analysis to the optical microscope of the kidneys, heart, spleen, stomach and small intestine showed no changes in the structures of these bodies, despite the different treatments. Animals fed the AIN-93 diet, regardless of age, had hepatic steatosis in frequency significantly higher than the animals that received the commercial Nuvilab®. The different diets did not cause influence on the absolute and relative weights of organs of animals, except for the absolute weight of the liver among younger animals and relative weight of the intestine among older animals. There was no influence of different diets on biochemical parameters evaluated, and the differences detected possibly resulting from the interaction between age and length of exposure of animals to diets. The markers of damage of kidney and liver function were similar and serum creatinine varied according to age. It was shown that both diets, AIN-93 and Nuvilab ®, are able to promote the growth of rats for a period of study considered subchronic. However, the occurrence of hepatic steatosis in animals fed the AIN-93 diet in pellets, reinforces the importance of tracking the standard protocols of experimentation and is indicative of nutritional inadequacies by imposing the need for further investigations to clarify which components or characteristics of this diet, widely used in animal experiments, may have contributed to this result. For instance, it is suggested that diets in the form of flour are used preferably those pellets, particularly on protocols to investigate metabolic effects. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-11-12 2010-01-22 2015-08-25T18:37:14Z 2015-08-25T18:37:14Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/masterThesis |
format |
masterThesis |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
BARBOSA, Junia Helena Porto. Standard diets used in animal experimentation : a comparative analysis.. 2008. 78 f. Dissertação (Mestrado em Nutrição) - Universidade Federal de Alagoas, Maceió, 2008. http://repositorio.ufal.br/handle/riufal/637 |
identifier_str_mv |
BARBOSA, Junia Helena Porto. Standard diets used in animal experimentation : a comparative analysis.. 2008. 78 f. Dissertação (Mestrado em Nutrição) - Universidade Federal de Alagoas, Maceió, 2008. |
url |
http://repositorio.ufal.br/handle/riufal/637 |
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por |
language |
por |
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info:eu-repo/semantics/embargoedAccess |
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embargoedAccess |
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application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade Federal de Alagoas BR Nutrição Programa de Pós-Graduação em Nutrição UFAL |
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Universidade Federal de Alagoas BR Nutrição Programa de Pós-Graduação em Nutrição UFAL |
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reponame:Repositório Institucional da Universidade Federal de Alagoas (UFAL) instname:Universidade Federal de Alagoas (UFAL) instacron:UFAL |
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Universidade Federal de Alagoas (UFAL) |
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UFAL |
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Repositório Institucional da Universidade Federal de Alagoas (UFAL) |
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Repositório Institucional da Universidade Federal de Alagoas (UFAL) |
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Repositório Institucional da Universidade Federal de Alagoas (UFAL) - Universidade Federal de Alagoas (UFAL) |
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ri@sibi.ufal.br |
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