Identificação de polimorfismos em genes codificantes de interferons em tambaqui (Colossoma macropomum)

• Autor(a) principal: Venancio, Aldenei Jumbata
• Data de Publicação: 2023
• Tipo de documento: Trabalho de conclusão de curso
• Idioma: por
• Título da fonte: Repositório Institucional da UFAM
• Texto Completo: http://riu.ufam.edu.br/handle/prefix/7818
• topic: Tambaqui; Interferon; Resposta imune; Polimorfismos genéticos; Doenças virais; CIENCIAS AGRARIAS; Tambaqui (Peixe); Peixes - Doenças; Peixes - Viveiros

The tambaqui stands out in Brazilian aquaculture due to its rapid growth, disease tolerance, and market popularity. Captive breeding has been expanding to meet the growing national and international demand. In aquaculture, viral diseases represent a significant concern, leading to intensive study of fish viruses and their antiviral responses, particularly in commercially valuable species. In teleosts, the immune response to viral infection includes the activation of interferon, crucial for antiviral defense. This study focuses on the genetic analysis of interferon-related genes, essential in fish immunity. Data from 424 tambaquis, both wild and from fish farms, from three distinct Brazilian regions were used. Starting from a database of 182,416 SNPs (Single Nucleotide Polymorphisms), alignment with the tambaqui genome using BLASTn identified 1,346 sequences related to interferon genes. A rigorous filter was applied, maintaining 79 markers in 368 animals, ensuring data quality. The alignments were categorized into four main groups: Interferon Regulatory Genes, Interferon Response-Associated Genes, IRF Binding Protein Genes, and Interferon Receptor Genes. The analysis indicated considerable diversity of interferon genes, suggesting a complex immune response. This study contributes to aquaculture by providing valuable insights to improve disease resistance in tambaqui and highlights the need for additional research for a deeper understanding of the genetic implications and breeding of these findings.