Evaluation of PCR and multiplex PCR in relation to nested PCR for diagnosing Theileria equi

Detalhes bibliográficos
Autor(a) principal: Leal, Danielle C.
Data de Publicação: 2011
Outros Autores: Madruga, Cláudio R., Matos, Paulo F. de, Souza, Bárbara Maria Paraná da Silva, Franke, Carlos R.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/16467
Resumo: p. 575-578
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spelling Leal, Danielle C.Madruga, Cláudio R.Matos, Paulo F. deSouza, Bárbara Maria Paraná da SilvaFranke, Carlos R.2014-10-27T22:09:19Z2014-10-27T22:09:19Z20110100-736Xhttp://repositorio.ufba.br/ri/handle/ri/16467v. 31, n. 7p. 575-578Conventional PCR (PCRTeq) for diagnosing Theileria equi and multiplex PCR (M/PCRTeq-Bc) for diagnosing T. equi and Babesia caballi were comparatively evaluated with nested PCR (N/PCR-Teq) for diagnosing equine piroplasmosis. In DNA sensitivity determinations, in multiple dilutions of equine blood that had tested positive for T. equi, PCR-Teq and N/PCR-Teq detected hemoparasite DNA in the larger dilutions (1:128), but did not differ significantly from the M/PCRTeq-Bc (1:64). In analyses on equine serum tested by ELISA, there was high agreement between this serological test and PCR-Teq (k = 0.780) and moderate agreement with N/PCR-Teq (k = 0.562) and M/PCRTeq-Bc (k = 0.488). PCR-Teq found a higher frequency of T. equi both in extensively and intensively reared horses, but this was not significant in relation to N/PCR-Teq (P>0.05), and both PCRs indicated that there was an endemic situation regarding T. equi in the population of horses of this sample. PCR-Teq was only significantly different from M/PCR-Teq-Bc (P<0.05). PCR-Teq presented high sensitivity and specificity, comparable to N/PCR-Teq, but with the advantage of higher speed in obtaining results and lower costs and risks of laboratory contamination. This accredits PCR-Teq for epidemiological studies and for determinations on affected horses.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-03-13T17:06:21Z No. of bitstreams: 1 Danielle C. Leal.pdf: 440032 bytes, checksum: c801f81bb335781ac6edfc0971cef8e8 (MD5)Approved for entry into archive by Alda Lima da Silva (sivalda@ufba.br) on 2014-10-27T22:09:19Z (GMT) No. of bitstreams: 1 Danielle C. Leal.pdf: 440032 bytes, checksum: c801f81bb335781ac6edfc0971cef8e8 (MD5)Made available in DSpace on 2014-10-27T22:09:19Z (GMT). No. of bitstreams: 1 Danielle C. Leal.pdf: 440032 bytes, checksum: c801f81bb335781ac6edfc0971cef8e8 (MD5) Previous issue date: 2011http://dx.doi.org/10.1590/S0100-736X2011000700005reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBATheileria equiPCRMultiplex PCRHorsesEquinosEvaluation of PCR and multiplex PCR in relation to nested PCR for diagnosing Theileria equiPesquisa Veterinária Brasileirainfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessengORIGINALDanielle C. Leal.pdfDanielle C. 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dc.title.pt_BR.fl_str_mv Evaluation of PCR and multiplex PCR in relation to nested PCR for diagnosing Theileria equi
dc.title.alternative.pt_BR.fl_str_mv Pesquisa Veterinária Brasileira
title Evaluation of PCR and multiplex PCR in relation to nested PCR for diagnosing Theileria equi
spellingShingle Evaluation of PCR and multiplex PCR in relation to nested PCR for diagnosing Theileria equi
Leal, Danielle C.
Theileria equi
PCR
Multiplex PCR
Horses
Equinos
title_short Evaluation of PCR and multiplex PCR in relation to nested PCR for diagnosing Theileria equi
title_full Evaluation of PCR and multiplex PCR in relation to nested PCR for diagnosing Theileria equi
title_fullStr Evaluation of PCR and multiplex PCR in relation to nested PCR for diagnosing Theileria equi
title_full_unstemmed Evaluation of PCR and multiplex PCR in relation to nested PCR for diagnosing Theileria equi
title_sort Evaluation of PCR and multiplex PCR in relation to nested PCR for diagnosing Theileria equi
author Leal, Danielle C.
author_facet Leal, Danielle C.
Madruga, Cláudio R.
Matos, Paulo F. de
Souza, Bárbara Maria Paraná da Silva
Franke, Carlos R.
author_role author
author2 Madruga, Cláudio R.
Matos, Paulo F. de
Souza, Bárbara Maria Paraná da Silva
Franke, Carlos R.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Leal, Danielle C.
Madruga, Cláudio R.
Matos, Paulo F. de
Souza, Bárbara Maria Paraná da Silva
Franke, Carlos R.
dc.subject.por.fl_str_mv Theileria equi
PCR
Multiplex PCR
Horses
Equinos
topic Theileria equi
PCR
Multiplex PCR
Horses
Equinos
description p. 575-578
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-10-27T22:09:19Z
dc.date.available.fl_str_mv 2014-10-27T22:09:19Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/16467
dc.identifier.issn.none.fl_str_mv 0100-736X
dc.identifier.number.pt_BR.fl_str_mv v. 31, n. 7
identifier_str_mv 0100-736X
v. 31, n. 7
url http://repositorio.ufba.br/ri/handle/ri/16467
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1590/S0100-736X2011000700005
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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