Experimental infection of dogs (Canis familiaris) with sporulated oocysts of Neospora caninum

Detalhes bibliográficos
Autor(a) principal: Bandini, Luciana Ahlf
Data de Publicação: 2011
Outros Autores: Alves Neto, Aldo Francisco, Pena, Hilda Fátima de Jesus, Cavalcante, Guacyara Tenório, Schares, Gereon, Nishi, Sandra Mayumi, Gennari, Solange Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/5524
Resumo: Acesso restrito: Texto completo. p. 151-156.
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spelling Bandini, Luciana AhlfAlves Neto, Aldo FranciscoPena, Hilda Fátima de JesusCavalcante, Guacyara TenórioSchares, GereonNishi, Sandra MayumiGennari, Solange MariaBandini, Luciana AhlfAlves Neto, Aldo FranciscoPena, Hilda Fátima de JesusCavalcante, Guacyara TenórioSchares, GereonNishi, Sandra MayumiGennari, Solange Maria2012-03-09T13:09:36Z20110304-4017http://www.repositorio.ufba.br/ri/handle/ri/5524v. 176, n. 2-3.Acesso restrito: Texto completo. p. 151-156.Neospora caninum is widely distributed in the world and this parasite is one of the major causes of abortion in cattle. Dogs and coyotes are definitive hosts of N. caninum and several species of domestic and wild animals are intermediate hosts. Dogs can become infected by the ingestion of tissues containing cysts and then excrete oocysts. It is not yet known whether sporulated oocysts are able to induce a patent infection in dogs, i.e. a shedding of N. caninum oocysts in feces. The objective of this study was to experimentally examine the infection of dogs by sporulated oocysts. The oocysts used in the experiment were obtained by feeding dogs with brain of buffaloes (Bubalus bubalis) positive for antiN.caninum antibodies by indirect fluorescent antibody test (IFAT ≥200). Oocysts shed by these dogs were confirmed to be N. caninum by molecular methods and by bioassay in gerbils, and sporulated N. caninum oocysts were used for the oral infection of four dogs. The dogs were 8 weeks old and negative for antibodies to N. caninum and Toxoplasma gondii. Dogs 1 and 4 received an inoculum of 10,000 sporulated oocysts each; dog 2 an inoculum of 5000 sporulated oocysts and dog 3 received 1000 sporulated oocysts of N. caninum. The total feces excreted by these dogs were collected and examined daily for a period of 30 days. No oocysts were found in their feces. The dogs were monitored monthly for a 6month period to observe a possible seroconversion and when this occurred the animals were eliminated from the experiment. Dogs 1 and 4 seroconverted 1 month after the infection with titer, in the IFAT, of 1600 and 800, respectively; the other two dogs presented no seroconvertion during the 6month period. Dogs 1 and 2 were euthanized 180 days after infection and were examined for the detection of N. caninum in tissues (brain, muscle, lymph node, liver, lung, heart and bone marrow) by immunohistochemistry and PCR with negative results in both techniques. Bioassay in gerbils with brain of these dogs was also performed and again the results were negative. In conclusion, dogs infected with sporulated oocysts of N. caninum were not able to shed oocysts in feces. However, a higher dose of infection stimulated the production of antibodies against N. caninum in the dogs.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-03-09T13:09:36Z No. of bitstreams: 1 __pdn.sciencedirect.com_....0-S0304401710006126-main.pdf: 163280 bytes, checksum: 0b7686b48ee4557deabf11d4f8b5fe03 (MD5)Made available in DSpace on 2012-03-09T13:09:36Z (GMT). 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dc.title.pt_BR.fl_str_mv Experimental infection of dogs (Canis familiaris) with sporulated oocysts of Neospora caninum
dc.title.alternative.pt_BR.fl_str_mv VETERINARY PARASITOLOGY
title Experimental infection of dogs (Canis familiaris) with sporulated oocysts of Neospora caninum
spellingShingle Experimental infection of dogs (Canis familiaris) with sporulated oocysts of Neospora caninum
Bandini, Luciana Ahlf
Neospora caninum
Sporulated oocysts
Experimental infection
Dogs
title_short Experimental infection of dogs (Canis familiaris) with sporulated oocysts of Neospora caninum
title_full Experimental infection of dogs (Canis familiaris) with sporulated oocysts of Neospora caninum
title_fullStr Experimental infection of dogs (Canis familiaris) with sporulated oocysts of Neospora caninum
title_full_unstemmed Experimental infection of dogs (Canis familiaris) with sporulated oocysts of Neospora caninum
title_sort Experimental infection of dogs (Canis familiaris) with sporulated oocysts of Neospora caninum
author Bandini, Luciana Ahlf
author_facet Bandini, Luciana Ahlf
Alves Neto, Aldo Francisco
Pena, Hilda Fátima de Jesus
Cavalcante, Guacyara Tenório
Schares, Gereon
Nishi, Sandra Mayumi
Gennari, Solange Maria
author_role author
author2 Alves Neto, Aldo Francisco
Pena, Hilda Fátima de Jesus
Cavalcante, Guacyara Tenório
Schares, Gereon
Nishi, Sandra Mayumi
Gennari, Solange Maria
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bandini, Luciana Ahlf
Alves Neto, Aldo Francisco
Pena, Hilda Fátima de Jesus
Cavalcante, Guacyara Tenório
Schares, Gereon
Nishi, Sandra Mayumi
Gennari, Solange Maria
Bandini, Luciana Ahlf
Alves Neto, Aldo Francisco
Pena, Hilda Fátima de Jesus
Cavalcante, Guacyara Tenório
Schares, Gereon
Nishi, Sandra Mayumi
Gennari, Solange Maria
dc.subject.por.fl_str_mv Neospora caninum
Sporulated oocysts
Experimental infection
Dogs
topic Neospora caninum
Sporulated oocysts
Experimental infection
Dogs
description Acesso restrito: Texto completo. p. 151-156.
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2012-03-09T13:09:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/5524
dc.identifier.issn.none.fl_str_mv 0304-4017
dc.identifier.number.pt_BR.fl_str_mv v. 176, n. 2-3.
identifier_str_mv 0304-4017
v. 176, n. 2-3.
url http://www.repositorio.ufba.br/ri/handle/ri/5524
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv DOI: 10.1016/j.vetpar.2010.10.047
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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instname_str Universidade Federal da Bahia (UFBA)
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