Proteomics of dedifferentiation of SK-N-BE2 neuroblastoma cells

Detalhes bibliográficos
Autor(a) principal: Saini, Ravi Kanth Rao
Data de Publicação: 2014
Outros Autores: Attarha, Sanaz, Santos, Claire da Silva, Kolakowska, Justyna, Funa, Keiko, Souchelnytskyi, Serhiy
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/17297
Resumo: Texto completo: acesso restrito. p.202–209
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spelling Saini, Ravi Kanth RaoAttarha, SanazSantos, Claire da SilvaKolakowska, JustynaFuna, KeikoSouchelnytskyi, SerhiySaini, Ravi Kanth RaoAttarha, SanazSantos, Claire da SilvaKolakowska, JustynaFuna, KeikoSouchelnytskyi, Serhiy2015-03-30T11:56:06Z2015-03-30T11:56:06Z2014http://repositorio.ufba.br/ri/handle/ri/17297v. 454, n. 1Texto completo: acesso restrito. p.202–209Neuroblastoma develops through processes which include cellular dedifferentiation. Ability of tumors to form spheroids is one of the manifestations of dedifferentiation and carcinogenic transformation. To study mechanisms of dedifferentiation of neuroblastoma cells, we generated spheroids and performed a proteomics study to compare the spheroids with parental SK-N-BE2 cells. We observed that dedifferentiation induced extensive changes in the proteome profiles of the cells, which affected more than 30% of detected cellular proteins. Using mass spectrometry, we identified 239 proteins affected by dedifferentiation into spheroids as compared to the parental cells. These proteins represented such regulatory processes as transcription, cell cycle regulation, apoptosis, cell adhesion, metabolism, intracellular transport, stress response, and angiogenesis. A number of potent regulators of stemness, differentiation and cancer were detected as subnetworks formed by the identified proteins. Our validation tissue microarray study of 30 neuroblastoma cases confirmed that two of the identified proteins, DISC1 and DNA-PKcs, had their expression increased in advanced malignancies. Thus, our report unveiled extensive changes of the cellular proteome upon dedifferentiation of neuroblastoma cells, indicated top subnetworks and clusters of molecular mechanisms involved in dedifferentiation, and provided candidate biomarkers for clinical studies.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2015-03-25T14:53:45Z No. of bitstreams: 1 Ravi Kanth Rao Saini.pdf: 2117613 bytes, checksum: 95cbb30ce4af03fc5ef8925bde8aeb65 (MD5)Approved for entry into archive by Delba Rosa (delba@ufba.br) on 2015-03-30T11:56:06Z (GMT) No. of bitstreams: 1 Ravi Kanth Rao Saini.pdf: 2117613 bytes, checksum: 95cbb30ce4af03fc5ef8925bde8aeb65 (MD5)Made available in DSpace on 2015-03-30T11:56:06Z (GMT). No. of bitstreams: 1 Ravi Kanth Rao Saini.pdf: 2117613 bytes, checksum: 95cbb30ce4af03fc5ef8925bde8aeb65 (MD5) Previous issue date: 2014http://dx.doi.org/10.1016/j.bbrc.2014.10.065reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBANeuroblastomaDedifferentiationProteomicsDISC1DNA-PKcsProteomics of dedifferentiation of SK-N-BE2 neuroblastoma cellsBiochemical and Biophysical Research Communicationsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessengORIGINALRavi Kanth Rao Saini.pdfRavi Kanth Rao Saini.pdfapplication/pdf2117613https://repositorio.ufba.br/bitstream/ri/17297/1/Ravi%20Kanth%20Rao%20Saini.pdf95cbb30ce4af03fc5ef8925bde8aeb65MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/17297/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52TEXTRavi Kanth Rao Saini.pdf.txtRavi Kanth Rao Saini.pdf.txtExtracted texttext/plain35268https://repositorio.ufba.br/bitstream/ri/17297/3/Ravi%20Kanth%20Rao%20Saini.pdf.txt0a65e16ed5e4d1b3673a65be146ad7b3MD53ri/172972022-08-08 13:20:03.751oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:20:03Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Proteomics of dedifferentiation of SK-N-BE2 neuroblastoma cells
dc.title.alternative.pt_BR.fl_str_mv Biochemical and Biophysical Research Communications
title Proteomics of dedifferentiation of SK-N-BE2 neuroblastoma cells
spellingShingle Proteomics of dedifferentiation of SK-N-BE2 neuroblastoma cells
Saini, Ravi Kanth Rao
Neuroblastoma
Dedifferentiation
Proteomics
DISC1
DNA-PKcs
title_short Proteomics of dedifferentiation of SK-N-BE2 neuroblastoma cells
title_full Proteomics of dedifferentiation of SK-N-BE2 neuroblastoma cells
title_fullStr Proteomics of dedifferentiation of SK-N-BE2 neuroblastoma cells
title_full_unstemmed Proteomics of dedifferentiation of SK-N-BE2 neuroblastoma cells
title_sort Proteomics of dedifferentiation of SK-N-BE2 neuroblastoma cells
author Saini, Ravi Kanth Rao
author_facet Saini, Ravi Kanth Rao
Attarha, Sanaz
Santos, Claire da Silva
Kolakowska, Justyna
Funa, Keiko
Souchelnytskyi, Serhiy
author_role author
author2 Attarha, Sanaz
Santos, Claire da Silva
Kolakowska, Justyna
Funa, Keiko
Souchelnytskyi, Serhiy
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Saini, Ravi Kanth Rao
Attarha, Sanaz
Santos, Claire da Silva
Kolakowska, Justyna
Funa, Keiko
Souchelnytskyi, Serhiy
Saini, Ravi Kanth Rao
Attarha, Sanaz
Santos, Claire da Silva
Kolakowska, Justyna
Funa, Keiko
Souchelnytskyi, Serhiy
dc.subject.por.fl_str_mv Neuroblastoma
Dedifferentiation
Proteomics
DISC1
DNA-PKcs
topic Neuroblastoma
Dedifferentiation
Proteomics
DISC1
DNA-PKcs
description Texto completo: acesso restrito. p.202–209
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-03-30T11:56:06Z
dc.date.available.fl_str_mv 2015-03-30T11:56:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/17297
dc.identifier.number.pt_BR.fl_str_mv v. 454, n. 1
url http://repositorio.ufba.br/ri/handle/ri/17297
identifier_str_mv v. 454, n. 1
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.bbrc.2014.10.065
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
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instname_str Universidade Federal da Bahia (UFBA)
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