Ultrastructural and immunocytochemical analyses of osteopontin in reactionary and reparative dentine formed after extrusion of upper rat incisors

Detalhes bibliográficos
Autor(a) principal: Aguiar, Márcio Cajazeira
Data de Publicação: 2007
Outros Autores: Arana Chavez, Victor E.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/14553
Resumo: Texto completo: acesso restrito. p. 418-427
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spelling Aguiar, Márcio CajazeiraArana Chavez, Victor E.Aguiar, Márcio CajazeiraArana Chavez, Victor E.2014-02-06T21:10:36Z20070021-8782http://repositorio.ufba.br/ri/handle/ri/14553v. 210, n. 4Texto completo: acesso restrito. p. 418-427Reactionary dentine and reparative dentine are two strategies used by the dentine–pulp complex to respond to injury. The reactionary dentine is secreted by original odontoblasts, while the reparative dentine is formed by odontoblast-like cells. Osteopontin (OPN) is a non-collagenous protein usually present in the repair of mineralized tissues. It is likely to be present in newly formed dentine but there are no studies attempting to detect it in reactionary and reparative dentine. The aim of the present study was to examine the ultrastructural characteristics, as well as the presence and distribution of OPN in reactionary and reparative dentine by provoking extrusion of the rat incisor. The right upper incisors of 3-month-old male rats were extruded 3 mm and then repositioned into their original sockets. At 3, 7, 10, 15, 20, 30 and 60 days after surgery, the incisors were fixed in glutaraldehyde–formaldehyde and then processed for scanning and transmission electron microscopy and for immunocytochemistry for OPN. After extrusive trauma, the dentine–pulp interface showed the presence of reactionary and reparative dentine, which varied in aspect, thickness and related cells. OPN was not detected in the physiological and reactionary dentine, while it was strongly immunoreactive in the matrix that surrounded the entrapped cells of reparative dentine. In addition, original odontoblasts subjacent to the physiological dentine contained OPN in their Golgi region. The present findings showed that reparative dentine shares some structural characteristics with primary bone, especially in relation to its OPN content. The odontoblast-like cells resemble osteoblasts rather than odontoblasts.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-01-31T13:06:14Z No. of bitstreams: 1 j.1469-7580.2007.00708.x.pdf: 328963 bytes, checksum: 346a5701d4f5ce08a58588491b733a55 (MD5)Approved for entry into archive by Patricia Barroso (pbarroso@ufba.br) on 2014-02-06T21:10:36Z (GMT) No. of bitstreams: 1 j.1469-7580.2007.00708.x.pdf: 328963 bytes, checksum: 346a5701d4f5ce08a58588491b733a55 (MD5)Made available in DSpace on 2014-02-06T21:10:36Z (GMT). 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dc.title.pt_BR.fl_str_mv Ultrastructural and immunocytochemical analyses of osteopontin in reactionary and reparative dentine formed after extrusion of upper rat incisors
dc.title.alternative.pt_BR.fl_str_mv Journal of Anatomy
title Ultrastructural and immunocytochemical analyses of osteopontin in reactionary and reparative dentine formed after extrusion of upper rat incisors
spellingShingle Ultrastructural and immunocytochemical analyses of osteopontin in reactionary and reparative dentine formed after extrusion of upper rat incisors
Aguiar, Márcio Cajazeira
Dental pulp
Immunocytochemistry
Odontoblast
Osteopontin
Reactionary dentine
Reparative dentine
Tertiary dentine
title_short Ultrastructural and immunocytochemical analyses of osteopontin in reactionary and reparative dentine formed after extrusion of upper rat incisors
title_full Ultrastructural and immunocytochemical analyses of osteopontin in reactionary and reparative dentine formed after extrusion of upper rat incisors
title_fullStr Ultrastructural and immunocytochemical analyses of osteopontin in reactionary and reparative dentine formed after extrusion of upper rat incisors
title_full_unstemmed Ultrastructural and immunocytochemical analyses of osteopontin in reactionary and reparative dentine formed after extrusion of upper rat incisors
title_sort Ultrastructural and immunocytochemical analyses of osteopontin in reactionary and reparative dentine formed after extrusion of upper rat incisors
author Aguiar, Márcio Cajazeira
author_facet Aguiar, Márcio Cajazeira
Arana Chavez, Victor E.
author_role author
author2 Arana Chavez, Victor E.
author2_role author
dc.contributor.author.fl_str_mv Aguiar, Márcio Cajazeira
Arana Chavez, Victor E.
Aguiar, Márcio Cajazeira
Arana Chavez, Victor E.
dc.subject.por.fl_str_mv Dental pulp
Immunocytochemistry
Odontoblast
Osteopontin
Reactionary dentine
Reparative dentine
Tertiary dentine
topic Dental pulp
Immunocytochemistry
Odontoblast
Osteopontin
Reactionary dentine
Reparative dentine
Tertiary dentine
description Texto completo: acesso restrito. p. 418-427
publishDate 2007
dc.date.issued.fl_str_mv 2007
dc.date.accessioned.fl_str_mv 2014-02-06T21:10:36Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/14553
dc.identifier.issn.none.fl_str_mv 0021-8782
dc.identifier.number.pt_BR.fl_str_mv v. 210, n. 4
identifier_str_mv 0021-8782
v. 210, n. 4
url http://repositorio.ufba.br/ri/handle/ri/14553
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
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