Characterization of pectinases from Acrophialophora nainiana and Trichoderma harzianum strain T6

Detalhes bibliográficos
Autor(a) principal: Celestino, Sonia Maria Costa
Data de Publicação: 2005
Outros Autores: Ferreira Filho, Edivaldo Ximenes
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/20410
Resumo: Art. original(p.97-181)
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spelling Celestino, Sonia Maria CostaFerreira Filho, Edivaldo XimenesCelestino, Sonia Maria CostaFerreira Filho, Edivaldo Ximenes2016-09-14T13:10:09Z2016-09-14T13:10:09Z2005CELESTINO, S. M. C.; FERREIRA FILHO, E. X.Characterization of pectinases from Acrophialophora nainiana and Trichoderma harzianum strain T6. R. Ci. méd. biol., Salvador, v. 4, n. 2, p. 97-104, mai./ago. 2005.2236-5222http://repositorio.ufba.br/ri/handle/ri/20410v.4, n.2Art. original(p.97-181)Two pectinase preparations have been isolated from liquid-state cultures of Acrophialophora nainiana and Trichoderma harzianum strain T6 containing pectin as the carbon source. Pectin lyase and pectin methylesterase activities were only detected in the enzyme preparations from A. nainiana. The exo-polymethylgalacturonase from A. nainiana was most active at pH 7.0 and 60oC, while exo-polymethylgalacturonase optimal activity from T. harzianum strain T6 was obtained at pH 4.3 and 40oC. Their stability at 40, 50 and 650C differed, being the exo-polymethylgalacturonase activity from A. nainiana more stable. Both enzyme preparations showed good stability at acidic pH. The crude extracts were partially purified by ultrafiltration, and gel filtration chromatography on Sephacryl S-100. Kinetic parameter studies showed that the lowest Km values were obtained with exo-polymethylgalacturonase from T. harzianum strain T6. The treatment of fruit juices with pectinase samples from A. nainiana and T. harzianum strain T6 resulted in a decrease of viscosity. Pectinase from T. harzianum strain T6 appeared to be more effective in the reduction of turbidity from apple juice. Only pectinase from T. harzianum strain T6 was able to extract juice from banana fruit.Submitted by ROBERTO PAULO CORREIA DE ARAÚJO (ppgorgsistem@ufba.br) on 2016-09-14T13:10:09Z No. of bitstreams: 1 1_v.4_2.pdf: 107378 bytes, checksum: fa36131f145350e8745b45a16ad28594 (MD5)Made available in DSpace on 2016-09-14T13:10:09Z (GMT). No. of bitstreams: 1 1_v.4_2.pdf: 107378 bytes, checksum: fa36131f145350e8745b45a16ad28594 (MD5) Previous issue date: 2005-05SalvadorInstituto de Ciências da Saúde/ Universidade Federal da Bahiahttp://www.portalseer.ufba.br/index.php/cmbio/article/view/4180/3057reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAPectin.Fungus.Enzyme characterization.Characterization of pectinases from Acrophialophora nainiana and Trichoderma harzianum strain T6Revista de Ciências Médicas e Biológicasinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessporORIGINAL1_v.4_2.pdf1_v.4_2.pdfapplication/pdf107378https://repositorio.ufba.br/bitstream/ri/20410/1/1_v.4_2.pdffa36131f145350e8745b45a16ad28594MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/20410/2/license.txtff6eaa8b858ea317fded99f125f5fcd0MD52TEXT1_v.4_2.pdf.txt1_v.4_2.pdf.txtExtracted texttext/plain23599https://repositorio.ufba.br/bitstream/ri/20410/3/1_v.4_2.pdf.txtdd03830474dc637ca142c13bdd39be87MD53ri/204102022-09-29 11:37:42.911oai:repositorio.ufba.br:ri/20410VGVybW8gZGUgTGljZW7vv71hLCBu77+9byBleGNsdXNpdm8sIHBhcmEgbyBkZXDvv71zaXRvIG5vIFJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRkJBLgoKIFBlbG8gcHJvY2Vzc28gZGUgc3VibWlzc++/vW8gZGUgZG9jdW1lbnRvcywgbyBhdXRvciBvdSBzZXUgcmVwcmVzZW50YW50ZSBsZWdhbCwgYW8gYWNlaXRhciAKZXNzZSB0ZXJtbyBkZSBsaWNlbu+/vWEsIGNvbmNlZGUgYW8gUmVwb3NpdO+/vXJpbyBJbnN0aXR1Y2lvbmFsIGRhIFVuaXZlcnNpZGFkZSBGZWRlcmFsIGRhIEJhaGlhIApvIGRpcmVpdG8gZGUgbWFudGVyIHVtYSBj77+9cGlhIGVtIHNldSByZXBvc2l077+9cmlvIGNvbSBhIGZpbmFsaWRhZGUsIHByaW1laXJhLCBkZSBwcmVzZXJ2Ye+/ve+/vW8uIApFc3NlcyB0ZXJtb3MsIG7vv71vIGV4Y2x1c2l2b3MsIG1hbnTvv71tIG9zIGRpcmVpdG9zIGRlIGF1dG9yL2NvcHlyaWdodCwgbWFzIGVudGVuZGUgbyBkb2N1bWVudG8gCmNvbW8gcGFydGUgZG8gYWNlcnZvIGludGVsZWN0dWFsIGRlc3NhIFVuaXZlcnNpZGFkZS4KCiBQYXJhIG9zIGRvY3VtZW50b3MgcHVibGljYWRvcyBjb20gcmVwYXNzZSBkZSBkaXJlaXRvcyBkZSBkaXN0cmlidWnvv73vv71vLCBlc3NlIHRlcm1vIGRlIGxpY2Vu77+9YSAKZW50ZW5kZSBxdWU6CgogTWFudGVuZG8gb3MgZGlyZWl0b3MgYXV0b3JhaXMsIHJlcGFzc2Fkb3MgYSB0ZXJjZWlyb3MsIGVtIGNhc28gZGUgcHVibGljYe+/ve+/vWVzLCBvIHJlcG9zaXTvv71yaW8KcG9kZSByZXN0cmluZ2lyIG8gYWNlc3NvIGFvIHRleHRvIGludGVncmFsLCBtYXMgbGliZXJhIGFzIGluZm9ybWHvv73vv71lcyBzb2JyZSBvIGRvY3VtZW50bwooTWV0YWRhZG9zIGVzY3JpdGl2b3MpLgoKIERlc3RhIGZvcm1hLCBhdGVuZGVuZG8gYW9zIGFuc2Vpb3MgZGVzc2EgdW5pdmVyc2lkYWRlIGVtIG1hbnRlciBzdWEgcHJvZHXvv73vv71vIGNpZW5077+9ZmljYSBjb20gCmFzIHJlc3Ryae+/ve+/vWVzIGltcG9zdGFzIHBlbG9zIGVkaXRvcmVzIGRlIHBlcmnvv71kaWNvcy4KCiBQYXJhIGFzIHB1YmxpY2Hvv73vv71lcyBzZW0gaW5pY2lhdGl2YXMgcXVlIHNlZ3VlbSBhIHBvbO+/vXRpY2EgZGUgQWNlc3NvIEFiZXJ0bywgb3MgZGVw77+9c2l0b3MgCmNvbXB1bHPvv71yaW9zIG5lc3NlIHJlcG9zaXTvv71yaW8gbWFudO+/vW0gb3MgZGlyZWl0b3MgYXV0b3JhaXMsIG1hcyBtYW5077+9bSBhY2Vzc28gaXJyZXN0cml0byAKYW8gbWV0YWRhZG9zIGUgdGV4dG8gY29tcGxldG8uIEFzc2ltLCBhIGFjZWl0Ye+/ve+/vW8gZGVzc2UgdGVybW8gbu+/vW8gbmVjZXNzaXRhIGRlIGNvbnNlbnRpbWVudG8KIHBvciBwYXJ0ZSBkZSBhdXRvcmVzL2RldGVudG9yZXMgZG9zIGRpcmVpdG9zLCBwb3IgZXN0YXJlbSBlbSBpbmljaWF0aXZhcyBkZSBhY2Vzc28gYWJlcnRvLgo=Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-09-29T14:37:42Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Characterization of pectinases from Acrophialophora nainiana and Trichoderma harzianum strain T6
dc.title.alternative.pt_BR.fl_str_mv Revista de Ciências Médicas e Biológicas
title Characterization of pectinases from Acrophialophora nainiana and Trichoderma harzianum strain T6
spellingShingle Characterization of pectinases from Acrophialophora nainiana and Trichoderma harzianum strain T6
Celestino, Sonia Maria Costa
Pectin.
Fungus.
Enzyme characterization.
title_short Characterization of pectinases from Acrophialophora nainiana and Trichoderma harzianum strain T6
title_full Characterization of pectinases from Acrophialophora nainiana and Trichoderma harzianum strain T6
title_fullStr Characterization of pectinases from Acrophialophora nainiana and Trichoderma harzianum strain T6
title_full_unstemmed Characterization of pectinases from Acrophialophora nainiana and Trichoderma harzianum strain T6
title_sort Characterization of pectinases from Acrophialophora nainiana and Trichoderma harzianum strain T6
author Celestino, Sonia Maria Costa
author_facet Celestino, Sonia Maria Costa
Ferreira Filho, Edivaldo Ximenes
author_role author
author2 Ferreira Filho, Edivaldo Ximenes
author2_role author
dc.contributor.author.fl_str_mv Celestino, Sonia Maria Costa
Ferreira Filho, Edivaldo Ximenes
Celestino, Sonia Maria Costa
Ferreira Filho, Edivaldo Ximenes
dc.subject.por.fl_str_mv Pectin.
Fungus.
Enzyme characterization.
topic Pectin.
Fungus.
Enzyme characterization.
description Art. original(p.97-181)
publishDate 2005
dc.date.issued.fl_str_mv 2005
dc.date.accessioned.fl_str_mv 2016-09-14T13:10:09Z
dc.date.available.fl_str_mv 2016-09-14T13:10:09Z
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dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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dc.identifier.citation.fl_str_mv CELESTINO, S. M. C.; FERREIRA FILHO, E. X.Characterization of pectinases from Acrophialophora nainiana and Trichoderma harzianum strain T6. R. Ci. méd. biol., Salvador, v. 4, n. 2, p. 97-104, mai./ago. 2005.
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/20410
dc.identifier.issn.none.fl_str_mv 2236-5222
dc.identifier.number.pt_BR.fl_str_mv v.4, n.2
identifier_str_mv CELESTINO, S. M. C.; FERREIRA FILHO, E. X.Characterization of pectinases from Acrophialophora nainiana and Trichoderma harzianum strain T6. R. Ci. méd. biol., Salvador, v. 4, n. 2, p. 97-104, mai./ago. 2005.
2236-5222
v.4, n.2
url http://repositorio.ufba.br/ri/handle/ri/20410
dc.language.iso.fl_str_mv por
language por
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Instituto de Ciências da Saúde/ Universidade Federal da Bahia
publisher.none.fl_str_mv Instituto de Ciências da Saúde/ Universidade Federal da Bahia
dc.source.pt_BR.fl_str_mv http://www.portalseer.ufba.br/index.php/cmbio/article/view/4180/3057
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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