SDS-PAGE and Western blot analysis of somatic and extracellular antigens of Corynebacterium pseudotuberculosis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2004 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/20345 |
Resumo: | Artigo original(p.44-52) |
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Paule, Bruno Jean AdrienAzevedo, Vasco Ariston de CarvalhoCosta, Lilia Ferreira MouraFreire, Songeli MenezesRegis, Lia FernandesVale, Vera Lúcia CostaBahia, Robson CerqueiraCarminati, RenatoNascimento, Ivana Lúcia de OliveiraNascimento, Roberto José Meyer2016-09-13T15:16:00Z2016-09-13T15:16:00Z2004-01PAULE, B. J. A. et aL. SDS-PAGE and Western blot analysis of somatic and extracellular antigens of Corynebacterium pseudotuberculosis. R. Ci. méd. biol., Salvador, v. 3, n. 1, p. 44-52, jan./jun. 2004.2236-5222http://repositorio.ufba.br/ri/handle/ri/20345v.3, n.1Artigo original(p.44-52)Different techniques to isolate secreted, cell-surface located and somatic fractions of Corynebacterium pseudotuberculosis have been studied by SDS-PAGE and immunoblotting, by using a pool of sera from naturally infected goats. The secreted antigens were obtained from culture supernatant of the bacteria grown in a chemically defined medium or in Brain Heart Infusion medium. The surface fraction was obtained by NaCl 1 M treatment and the somatic ones were obtained by using distinct procedures (detergents and ultrasounds). By Coomassie blue staining, we observed 20 bands in the secreted fraction, 35 bands in the surface extract and 40 to 50 bands in the somatic fraction, depending on the extraction procedure. Sixteen immunoreactive proteins were detected among the studied fractions. Bands with molecular weights of 125, 108, 75, 68, 41, 40, 31 and 24 kDa were recognized with higher intensity and all of them were found in the secreted fraction. The use of the chemically defined medium allowed us to evidence the presence of proteins with high molecular masses in the secreted fraction of C. pseudotuberculosis, which had not been previously described.Submitted by ROBERTO PAULO CORREIA DE ARAÚJO (ppgorgsistem@ufba.br) on 2016-09-13T15:16:00Z No. of bitstreams: 1 R. Ci. méd. biol., v.3, n. 1-2004.pdf: 192851 bytes, checksum: 745e388eeae5cbdcec61ee5969fa3a90 (MD5)Made available in DSpace on 2016-09-13T15:16:00Z (GMT). No. of bitstreams: 1 R. Ci. méd. biol., v.3, n. 1-2004.pdf: 192851 bytes, checksum: 745e388eeae5cbdcec61ee5969fa3a90 (MD5) Previous issue date: 2004-01SalvadorInstituto de Ciências da Saúde/ Universidade Federal da Bahiahttp://www.portalseer.ufba.br/index.php/cmbio/issue/view/498/showTocreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBACorynebacterium pseudotuberculosis.Goat.Antigens.SDS-PAGE.Western blot.SDS-PAGE and Western blot analysis of somatic and extracellular antigens of Corynebacterium pseudotuberculosisRevista de Ciências Médicas e Biológicasinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessporORIGINALR. Ci. méd. biol., v.3, n. 1-2004.pdfR. 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| dc.title.pt_BR.fl_str_mv |
SDS-PAGE and Western blot analysis of somatic and extracellular antigens of Corynebacterium pseudotuberculosis |
| dc.title.alternative.pt_BR.fl_str_mv |
Revista de Ciências Médicas e Biológicas |
| title |
SDS-PAGE and Western blot analysis of somatic and extracellular antigens of Corynebacterium pseudotuberculosis |
| spellingShingle |
SDS-PAGE and Western blot analysis of somatic and extracellular antigens of Corynebacterium pseudotuberculosis Paule, Bruno Jean Adrien Corynebacterium pseudotuberculosis. Goat. Antigens. SDS-PAGE. Western blot. |
| title_short |
SDS-PAGE and Western blot analysis of somatic and extracellular antigens of Corynebacterium pseudotuberculosis |
| title_full |
SDS-PAGE and Western blot analysis of somatic and extracellular antigens of Corynebacterium pseudotuberculosis |
| title_fullStr |
SDS-PAGE and Western blot analysis of somatic and extracellular antigens of Corynebacterium pseudotuberculosis |
| title_full_unstemmed |
SDS-PAGE and Western blot analysis of somatic and extracellular antigens of Corynebacterium pseudotuberculosis |
| title_sort |
SDS-PAGE and Western blot analysis of somatic and extracellular antigens of Corynebacterium pseudotuberculosis |
| author |
Paule, Bruno Jean Adrien |
| author_facet |
Paule, Bruno Jean Adrien Azevedo, Vasco Ariston de Carvalho Costa, Lilia Ferreira Moura Freire, Songeli Menezes Regis, Lia Fernandes Vale, Vera Lúcia Costa Bahia, Robson Cerqueira Carminati, Renato Nascimento, Ivana Lúcia de Oliveira Nascimento, Roberto José Meyer |
| author_role |
author |
| author2 |
Azevedo, Vasco Ariston de Carvalho Costa, Lilia Ferreira Moura Freire, Songeli Menezes Regis, Lia Fernandes Vale, Vera Lúcia Costa Bahia, Robson Cerqueira Carminati, Renato Nascimento, Ivana Lúcia de Oliveira Nascimento, Roberto José Meyer |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Paule, Bruno Jean Adrien Azevedo, Vasco Ariston de Carvalho Costa, Lilia Ferreira Moura Freire, Songeli Menezes Regis, Lia Fernandes Vale, Vera Lúcia Costa Bahia, Robson Cerqueira Carminati, Renato Nascimento, Ivana Lúcia de Oliveira Nascimento, Roberto José Meyer |
| dc.subject.por.fl_str_mv |
Corynebacterium pseudotuberculosis. Goat. Antigens. SDS-PAGE. Western blot. |
| topic |
Corynebacterium pseudotuberculosis. Goat. Antigens. SDS-PAGE. Western blot. |
| description |
Artigo original(p.44-52) |
| publishDate |
2004 |
| dc.date.issued.fl_str_mv |
2004-01 |
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2016-09-13T15:16:00Z |
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2016-09-13T15:16:00Z |
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PAULE, B. J. A. et aL. SDS-PAGE and Western blot analysis of somatic and extracellular antigens of Corynebacterium pseudotuberculosis. R. Ci. méd. biol., Salvador, v. 3, n. 1, p. 44-52, jan./jun. 2004. |
| dc.identifier.uri.fl_str_mv |
http://repositorio.ufba.br/ri/handle/ri/20345 |
| dc.identifier.issn.none.fl_str_mv |
2236-5222 |
| dc.identifier.number.pt_BR.fl_str_mv |
v.3, n.1 |
| identifier_str_mv |
PAULE, B. J. A. et aL. SDS-PAGE and Western blot analysis of somatic and extracellular antigens of Corynebacterium pseudotuberculosis. R. Ci. méd. biol., Salvador, v. 3, n. 1, p. 44-52, jan./jun. 2004. 2236-5222 v.3, n.1 |
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Instituto de Ciências da Saúde/ Universidade Federal da Bahia |
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Instituto de Ciências da Saúde/ Universidade Federal da Bahia |
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http://www.portalseer.ufba.br/index.php/cmbio/issue/view/498/showToc |
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