Optimization and validation of a method for the direct determination of catechin and epicatechin in red wines by HPLC/fluorescence

Detalhes bibliográficos
Autor(a) principal: Dias, Fábio de Souza
Data de Publicação: 2010
Outros Autores: Lovillo, Miguel Palma, Barroso, Carmelo Garcia, David, Jorge Mauricio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/5619
Resumo: Acesso restrito: Texto completo. p. 17-20.
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spelling Dias, Fábio de SouzaLovillo, Miguel PalmaBarroso, Carmelo GarciaDavid, Jorge MauricioDias, Fábio de SouzaLovillo, Miguel PalmaBarroso, Carmelo GarciaDavid, Jorge Mauricio2012-04-13T13:03:45Z20100026-265Xhttp://www.repositorio.ufba.br/ri/handle/ri/5619v. 96, n. 1.Acesso restrito: Texto completo. p. 17-20.The present paper describes a direct procedure for the determination of catechin and epicatechin concentrations in red wines employing reverse-phase high performance liquid chromatography (RP HPLC) and detection by fluorescence. The method was performed using a sample volume of 10 μL without dilution. The separation process employed a Chromolith performance RP-18e (100 mm×4.6 mm) column, and the mobile phase was composed of solvent A: methanol–acetic acid–water (90:8:2) and solvent B: water–acetic acid–methanol (10:2:88) at a flow rate of 1.0 mL min−1. Linearity was observed in the range of 1 to 30 mg L−1, with limits of detection and quantification of 0.27 and 0.89 mg L−1, respectively, for catechin and 0.33 and 1.01 mg L−1, respectively, for epicatechin. The precisions estimated by the relative standard deviation were 3.34 and 1.09% for catechin concentrations of 0.5 and 20 mg L−1 respectively and 2.82 and 0.49% for epicatechin concentrations of 0.5 and 20 mg L−1, respectively. The evaluation of the accuracy was done using an addition/recovery assay. Four wine samples were used, and the recoveries varied from 105 to 108% for catechin and from 97 to 119% for epicatechin. The method was applied to the analysis of red wine samples collected from the São Francisco region, Bahia State, Brazil. Nine samples were analyzed, and the catechin and epicatechin concentrations varied from 7.51 to 73.20 and from 5.08 to 43.32 mg L−1, respectively. The concentrations found agree with data reported in the literature.Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-04-13T13:03:45Z No. of bitstreams: 1 __ac.els-cdn.com_S002626...5c8c1d8ed0d16d2304794fdf7447c.pdf: 189779 bytes, checksum: c7ea873bba7648d705fdee1a984bcf35 (MD5)Made available in DSpace on 2012-04-13T13:03:45Z (GMT). No. of bitstreams: 1 __ac.els-cdn.com_S002626...5c8c1d8ed0d16d2304794fdf7447c.pdf: 189779 bytes, checksum: c7ea873bba7648d705fdee1a984bcf35 (MD5) Previous issue date: 2010DOI: 10.1016/j.microc.2010.01.004reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBACatechinEpicatechinRed wineHPLC/fluorescenceOptimization and validation of a method for the direct determination of catechin and epicatechin in red wines by HPLC/fluorescenceMicrochemical Journalinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINAL__ac.els-cdn.com_S002626...5c8c1d8ed0d16d2304794fdf7447c.pdf__ac.els-cdn.com_S002626...5c8c1d8ed0d16d2304794fdf7447c.pdfapplication/pdf189779https://repositorio.ufba.br/bitstream/ri/5619/1/__ac.els-cdn.com_S002626...5c8c1d8ed0d16d2304794fdf7447c.pdfc7ea873bba7648d705fdee1a984bcf35MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/5619/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT__ac.els-cdn.com_S002626...5c8c1d8ed0d16d2304794fdf7447c.pdf.txt__ac.els-cdn.com_S002626...5c8c1d8ed0d16d2304794fdf7447c.pdf.txtExtracted texttext/plain20302https://repositorio.ufba.br/bitstream/ri/5619/3/__ac.els-cdn.com_S002626...5c8c1d8ed0d16d2304794fdf7447c.pdf.txt3f61a0c71e496bd816724eae28f6fad0MD53ri/56192022-07-05 14:03:21.039oai:repositorio.ufba.br:ri/5619VGVybW8gZGUgTGljZW7vv71hLCBu77+9byBleGNsdXNpdm8sIHBhcmEgbyBkZXDvv71zaXRvIG5vIHJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRkJBCgogICAgUGVsbyBwcm9jZXNzbyBkZSBzdWJtaXNz77+9byBkZSBkb2N1bWVudG9zLCBvIGF1dG9yIG91IHNldQpyZXByZXNlbnRhbnRlIGxlZ2FsLCBhbyBhY2VpdGFyIGVzc2UgdGVybW8gZGUgbGljZW7vv71hLCBjb25jZWRlIGFvClJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkYSBCYWhpYSBvIGRpcmVpdG8KZGUgbWFudGVyIHVtYSBj77+9cGlhIGVtIHNldSByZXBvc2l077+9cmlvIGNvbSBhIGZpbmFsaWRhZGUsIHByaW1laXJhLCAKZGUgcHJlc2VydmHvv73vv71vLiBFc3NlcyB0ZXJtb3MsIG7vv71vIGV4Y2x1c2l2b3MsIG1hbnTvv71tIG9zIGRpcmVpdG9zIGRlIAphdXRvci9jb3B5cmlnaHQsIG1hcyBlbnRlbmRlIG8gZG9jdW1lbnRvIGNvbW8gcGFydGUgZG8gYWNlcnZvIGludGVsZWN0dWFsIGRlc3NhIFVuaXZlcnNpZGFkZS4gCgogICAgUGFyYSBvcyBkb2N1bWVudG9zIHB1YmxpY2Fkb3MgY29tIHJlcGFzc2UgZGUgZGlyZWl0b3MgZGUgZGlzdHJpYnVp77+977+9bywgZXNzZSB0ZXJtbyBkZSBsaWNlbu+/vWEgZW50ZW5kZSBxdWU6IAoKICAgIE1hbnRlbmRvIG9zICBkaXJlaXRvcyBhdXRvcmFpcywgcmVwYXNzYWRvcyBhIHRlcmNlaXJvcywgZW0gY2FzbyAKZGUgcHVibGljYe+/ve+/vWVzLCBvIHJlcG9zaXTvv71yaW8gcG9kZSByZXN0cmluZ2lyIG8gYWNlc3NvIGFvIHRleHRvIAppbnRlZ3JhbCwgbWFzIGxpYmVyYSBhcyBpbmZvcm1h77+977+9ZXMgc29icmUgbyBkb2N1bWVudG8gKE1ldGFkYWRvcyBkZXNjcml0aXZvcykuCgogRGVzdGEgZm9ybWEsIGF0ZW5kZW5kbyBhb3MgYW5zZWlvcyBkZXNzYSB1bml2ZXJzaWRhZGUgCmVtIG1hbnRlciBzdWEgcHJvZHXvv73vv71vIGNpZW5077+9ZmljYSBjb20gYXMgcmVzdHJp77+977+9ZXMgaW1wb3N0YXMgcGVsb3MgCmVkaXRvcmVzIGRlIHBlcmnvv71kaWNvcy4gCgogICAgUGFyYSBhcyBwdWJsaWNh77+977+9ZXMgZW0gaW5pY2lhdGl2YXMgcXVlIHNlZ3VlbSBhIHBvbO+/vXRpY2EgZGUgCkFjZXNzbyBBYmVydG8sIG9zIGRlcO+/vXNpdG9zIGNvbXB1bHPvv71yaW9zIG5lc3NlIHJlcG9zaXTvv71yaW8gbWFudO+/vW0gCm9zIGRpcmVpdG9zIGF1dG9yYWlzLCBtYXMgbWFudO+/vW0gbyBhY2Vzc28gaXJyZXN0cml0byBhbyBtZXRhZGFkb3MgCmUgdGV4dG8gY29tcGxldG8uIEFzc2ltLCBhIGFjZWl0Ye+/ve+/vW8gZGVzc2UgdGVybW8gbu+/vW8gbmVjZXNzaXRhIGRlIApjb25zZW50aW1lbnRvIHBvciBwYXJ0ZSBkZSBhdXRvcmVzL2RldGVudG9yZXMgZG9zIGRpcmVpdG9zLCBwb3IgCmVzdGFyZW0gZW0gaW5pY2lhdGl2YXMgZGUgYWNlc3NvIGFiZXJ0by4KCiAgICBFbSBhbWJvcyBvIGNhc28sIGVzc2UgdGVybW8gZGUgbGljZW7vv71hLCBwb2RlIHNlciBhY2VpdG8gcGVsbyAKYXV0b3IsIGRldGVudG9yZXMgZGUgZGlyZWl0b3MgZS9vdSB0ZXJjZWlyb3MgYW1wYXJhZG9zIHBlbGEgCnVuaXZlcnNpZGFkZS4gRGV2aWRvIGFvcyBkaWZlcmVudGVzIHByb2Nlc3NvcyBwZWxvIHF1YWwgYSBzdWJtaXNz77+9byAKcG9kZSBvY29ycmVyLCBvIHJlcG9zaXTvv71yaW8gcGVybWl0ZSBhIGFjZWl0Ye+/ve+/vW8gZGEgbGljZW7vv71hIHBvciAKdGVyY2Vpcm9zLCBzb21lbnRlIG5vcyBjYXNvcyBkZSBkb2N1bWVudG9zIHByb2R1emlkb3MgcG9yIGludGVncmFudGVzIApkYSBVRkJBIGUgc3VibWV0aWRvcyBwb3IgcGVzc29hcyBhbXBhcmFkYXMgcG9yIGVzdGEgaW5zdGl0dWnvv73vv71vLgo=Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:21Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Optimization and validation of a method for the direct determination of catechin and epicatechin in red wines by HPLC/fluorescence
dc.title.alternative.pt_BR.fl_str_mv Microchemical Journal
title Optimization and validation of a method for the direct determination of catechin and epicatechin in red wines by HPLC/fluorescence
spellingShingle Optimization and validation of a method for the direct determination of catechin and epicatechin in red wines by HPLC/fluorescence
Dias, Fábio de Souza
Catechin
Epicatechin
Red wine
HPLC/fluorescence
title_short Optimization and validation of a method for the direct determination of catechin and epicatechin in red wines by HPLC/fluorescence
title_full Optimization and validation of a method for the direct determination of catechin and epicatechin in red wines by HPLC/fluorescence
title_fullStr Optimization and validation of a method for the direct determination of catechin and epicatechin in red wines by HPLC/fluorescence
title_full_unstemmed Optimization and validation of a method for the direct determination of catechin and epicatechin in red wines by HPLC/fluorescence
title_sort Optimization and validation of a method for the direct determination of catechin and epicatechin in red wines by HPLC/fluorescence
author Dias, Fábio de Souza
author_facet Dias, Fábio de Souza
Lovillo, Miguel Palma
Barroso, Carmelo Garcia
David, Jorge Mauricio
author_role author
author2 Lovillo, Miguel Palma
Barroso, Carmelo Garcia
David, Jorge Mauricio
author2_role author
author
author
dc.contributor.author.fl_str_mv Dias, Fábio de Souza
Lovillo, Miguel Palma
Barroso, Carmelo Garcia
David, Jorge Mauricio
Dias, Fábio de Souza
Lovillo, Miguel Palma
Barroso, Carmelo Garcia
David, Jorge Mauricio
dc.subject.por.fl_str_mv Catechin
Epicatechin
Red wine
HPLC/fluorescence
topic Catechin
Epicatechin
Red wine
HPLC/fluorescence
description Acesso restrito: Texto completo. p. 17-20.
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2012-04-13T13:03:45Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/5619
dc.identifier.issn.none.fl_str_mv 0026-265X
dc.identifier.number.pt_BR.fl_str_mv v. 96, n. 1.
identifier_str_mv 0026-265X
v. 96, n. 1.
url http://www.repositorio.ufba.br/ri/handle/ri/5619
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv DOI: 10.1016/j.microc.2010.01.004
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
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