Detection of Hammondia heydorni and related coccidia (Neospora caninum and Toxoplasma gondii) in goats slaughtered in Bahia, Brazil

Detalhes bibliográficos
Autor(a) principal: Silva, Mariana S. A.
Data de Publicação: 2009
Outros Autores: Uzêda, Rosângela Soares, Costa, Kattyanne S., Santos, Sara L., Macedo, Alan C. C., Abe Sandes, Kiyoko, Gondim, Luis Fernando Pita
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/13269
Resumo: Texto completo: acesso restrito. p. 156-159
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spelling Silva, Mariana S. A.Uzêda, Rosângela SoaresCosta, Kattyanne S.Santos, Sara L.Macedo, Alan C. C.Abe Sandes, KiyokoGondim, Luis Fernando PitaSilva, Mariana S. A.Uzêda, Rosângela SoaresCosta, Kattyanne S.Santos, Sara L.Macedo, Alan C. C.Abe Sandes, KiyokoGondim, Luis Fernando Pita2013-10-15T18:26:02Z20090304-4017http://www.repositorio.ufba.br/ri/handle/ri/13269v. 162, n. 1-2Texto completo: acesso restrito. p. 156-159Hammondia heydorni is a coccidian parasite with an obligatory two host life cycle, with dogs and foxes as definitive hosts, and a number of intermediate hosts, including goats. While infection by this parasite seems to be unassociated with any clinical signs, infection by the closely related parasites Neospora caninum and Toxoplasma gondii can result in abortion, stillbirths and low yielding in caprine herds. The aim of this work was to investigate the frequency of goats infected with H. heydorni using a nested PCR, specific to Toxoplasmatinae internal transcribed spacer 1 (ITS1) of the rDNA, followed by sequencing of the purified PCR fragments. The same molecular techniques were used to determine the frequencies of N. caninum and T. gondii-infected animals. A total frequency of 13.72% (14/102) was obtained for Toxoplasmatinae DNA in goat tissues. After sequencing the PCR products from all positive tissues, a frequency of 3.92% (4/102), 1.96% (2/102) and 7.84% (8/102) were obtained for H. heydorni, N. caninum and T. gondii, respectively. All sequences shared 98–100% identity with sequences from other strains of these coccidia present in GenBank. To the authors’ knowledge, this is the first report of H. heydorni DNA in tissues from naturally infected intermediate hosts.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-10-11T11:38:27Z No. of bitstreams: 1 1-s2.0-S0304401709001071-main.pdf: 132359 bytes, checksum: f3ecaf49ad6e7ac9f2d103d90d3da1ec (MD5)Approved for entry into archive by Alda Lima da Silva(sivalda@ufba.br) on 2013-10-15T18:26:02Z (GMT) No. of bitstreams: 1 1-s2.0-S0304401709001071-main.pdf: 132359 bytes, checksum: f3ecaf49ad6e7ac9f2d103d90d3da1ec (MD5)Made available in DSpace on 2013-10-15T18:26:02Z (GMT). No. of bitstreams: 1 1-s2.0-S0304401709001071-main.pdf: 132359 bytes, checksum: f3ecaf49ad6e7ac9f2d103d90d3da1ec (MD5) Previous issue date: 2009http://dx.doi.org/10.1016/j.vetpar.2009.02.007reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAHammondia heydorniNeospora caninumToxoplasma gondiiGoatsPCRToxoplasmatinaeDetection of Hammondia heydorni and related coccidia (Neospora caninum and Toxoplasma gondii) in goats slaughtered in Bahia, BrazilVeterinary Parasitologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINAL1-s2.0-S0304401709001071-main.pdf1-s2.0-S0304401709001071-main.pdfapplication/pdf132359https://repositorio.ufba.br/bitstream/ri/13269/1/1-s2.0-S0304401709001071-main.pdff3ecaf49ad6e7ac9f2d103d90d3da1ecMD51LICENSElicense.txtlicense.txttext/plain1365https://repositorio.ufba.br/bitstream/ri/13269/2/license.txt5371a150bdc863f78dcf39281543bd86MD52TEXT1-s2.0-S0304401709001071-main.pdf.txt1-s2.0-S0304401709001071-main.pdf.txtExtracted texttext/plain25304https://repositorio.ufba.br/bitstream/ri/13269/3/1-s2.0-S0304401709001071-main.pdf.txt473a23af081b1d22419dac41fab24798MD53ri/132692022-07-05 14:02:58.795oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:02:58Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Detection of Hammondia heydorni and related coccidia (Neospora caninum and Toxoplasma gondii) in goats slaughtered in Bahia, Brazil
dc.title.alternative.pt_BR.fl_str_mv Veterinary Parasitology
title Detection of Hammondia heydorni and related coccidia (Neospora caninum and Toxoplasma gondii) in goats slaughtered in Bahia, Brazil
spellingShingle Detection of Hammondia heydorni and related coccidia (Neospora caninum and Toxoplasma gondii) in goats slaughtered in Bahia, Brazil
Silva, Mariana S. A.
Hammondia heydorni
Neospora caninum
Toxoplasma gondii
Goats
PCR
Toxoplasmatinae
title_short Detection of Hammondia heydorni and related coccidia (Neospora caninum and Toxoplasma gondii) in goats slaughtered in Bahia, Brazil
title_full Detection of Hammondia heydorni and related coccidia (Neospora caninum and Toxoplasma gondii) in goats slaughtered in Bahia, Brazil
title_fullStr Detection of Hammondia heydorni and related coccidia (Neospora caninum and Toxoplasma gondii) in goats slaughtered in Bahia, Brazil
title_full_unstemmed Detection of Hammondia heydorni and related coccidia (Neospora caninum and Toxoplasma gondii) in goats slaughtered in Bahia, Brazil
title_sort Detection of Hammondia heydorni and related coccidia (Neospora caninum and Toxoplasma gondii) in goats slaughtered in Bahia, Brazil
author Silva, Mariana S. A.
author_facet Silva, Mariana S. A.
Uzêda, Rosângela Soares
Costa, Kattyanne S.
Santos, Sara L.
Macedo, Alan C. C.
Abe Sandes, Kiyoko
Gondim, Luis Fernando Pita
author_role author
author2 Uzêda, Rosângela Soares
Costa, Kattyanne S.
Santos, Sara L.
Macedo, Alan C. C.
Abe Sandes, Kiyoko
Gondim, Luis Fernando Pita
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Silva, Mariana S. A.
Uzêda, Rosângela Soares
Costa, Kattyanne S.
Santos, Sara L.
Macedo, Alan C. C.
Abe Sandes, Kiyoko
Gondim, Luis Fernando Pita
Silva, Mariana S. A.
Uzêda, Rosângela Soares
Costa, Kattyanne S.
Santos, Sara L.
Macedo, Alan C. C.
Abe Sandes, Kiyoko
Gondim, Luis Fernando Pita
dc.subject.por.fl_str_mv Hammondia heydorni
Neospora caninum
Toxoplasma gondii
Goats
PCR
Toxoplasmatinae
topic Hammondia heydorni
Neospora caninum
Toxoplasma gondii
Goats
PCR
Toxoplasmatinae
description Texto completo: acesso restrito. p. 156-159
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2013-10-15T18:26:02Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/13269
dc.identifier.issn.none.fl_str_mv 0304-4017
dc.identifier.number.pt_BR.fl_str_mv v. 162, n. 1-2
identifier_str_mv 0304-4017
v. 162, n. 1-2
url http://www.repositorio.ufba.br/ri/handle/ri/13269
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.vetpar.2009.02.007
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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