Corynebacterium pseudotuberculosis secreted antigen-induced specific gamma-interferon production by peripheral blood leukocytes: potential diagnostic marker for caseous lymphadenitis in sheep and goats
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/4873 |
Resumo: | p. 213–220 |
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Rebouças, Miriam FloresPortela, Ricardo Wagner DiasLima, Danielle DantasLoureiro, DanBastos, Bruno LopesCosta, Lília Ferreira de MouraVale, Vera Lúcia CostaMiyoshi, AndersonAzevedo, Vasco Ariston de CarvalhoNascimento, Roberto José MeyerRebouças, Miriam FloresPortela, Ricardo Wagner DiasLima, Danielle DantasLoureiro, DanBastos, Bruno LopesCosta, Lília Ferreira de MouraVale, Vera Lúcia CostaMiyoshi, AndersonAzevedo, Vasco Ariston de CarvalhoNascimento, Roberto José Meyer2011-12-16T12:25:46Z2011-12-16T12:25:46Z20111040-6387http://www.repositorio.ufba.br/ri/handle/ri/4873v. 23p. 213–220In the current study, the applicability of the quantification of gamma interferon (IFN-c) levels for the detection of animals infected with Corynebacterium pseudotuberculosis and for determining caseous lymphadenitis (CLA) clinical status was evaluated. Peripheral blood leukocytes were collected from CLA nonendemic areas animals, from CLA seropositive animals without clinical signs of the disease, and from seropositive animals presenting CLA clinical signs. The leukocytes were stimulated with C. pseudotuberculosis– secreted antigens that were concentrated by the three-phase partitioning technique. An ovine IFN-c enzymelinked immunosorbent assay was used to quantify IFN-c production. Goats and sheep with CLA had higher IFN-c levels than uninfected seronegative animals. Leukocytes from sheep with CLA chronic abscesses produced higher IFN-c levels when compared with seropositive sheep without CLA clinical signs, but this difference was not significant in goats. The sensitivity of the assay was 55.8% and 56%, whereas the specificity was 100% and 93%, for goats and sheep, respectively. In conclusion, IFN-c is a potential marker for the determination of CLA infection status in small ruminants; however, further research is needed to improve assay sensitivity.Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2011-12-16T12:25:46Z No. of bitstreams: 1 213.full.pdf: 567562 bytes, checksum: f6f339229ce9e5a210821762df7f7f09 (MD5)Made available in DSpace on 2011-12-16T12:25:46Z (GMT). No. of bitstreams: 1 213.full.pdf: 567562 bytes, checksum: f6f339229ce9e5a210821762df7f7f09 (MD5) Previous issue date: 2011Caseous lymphadenitisgoatssheepCorynebacterium pseudotuberculosisCorynebacterium pseudotuberculosis secreted antigen-induced specific gamma-interferon production by peripheral blood leukocytes: potential diagnostic marker for caseous lymphadenitis in sheep and goatsJournal of Veterinary Diagnostic Investigationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAinfo:eu-repo/semantics/openAccessORIGINAL213.full.pdf213.full.pdfapplication/pdf567562https://repositorio.ufba.br/bitstream/ri/4873/1/213.full.pdff6f339229ce9e5a210821762df7f7f09MD51LICENSElicense.txtlicense.txttext/plain1809https://repositorio.ufba.br/bitstream/ri/4873/2/license.txt8bb3e3eb871f5854a9ec419f68651b60MD52TEXT213.full.pdf.txt213.full.pdf.txtExtracted texttext/plain36682https://repositorio.ufba.br/bitstream/ri/4873/3/213.full.pdf.txt7473d828a25c77eeb82598fc2e4f360bMD53ri/48732022-07-05 14:03:20.085oai:repositorio.ufba.br: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ório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:20Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Corynebacterium pseudotuberculosis secreted antigen-induced specific gamma-interferon production by peripheral blood leukocytes: potential diagnostic marker for caseous lymphadenitis in sheep and goats |
| dc.title.alternative.pt_BR.fl_str_mv |
Journal of Veterinary Diagnostic Investigation |
| title |
Corynebacterium pseudotuberculosis secreted antigen-induced specific gamma-interferon production by peripheral blood leukocytes: potential diagnostic marker for caseous lymphadenitis in sheep and goats |
| spellingShingle |
Corynebacterium pseudotuberculosis secreted antigen-induced specific gamma-interferon production by peripheral blood leukocytes: potential diagnostic marker for caseous lymphadenitis in sheep and goats Rebouças, Miriam Flores Caseous lymphadenitis goats sheep Corynebacterium pseudotuberculosis |
| title_short |
Corynebacterium pseudotuberculosis secreted antigen-induced specific gamma-interferon production by peripheral blood leukocytes: potential diagnostic marker for caseous lymphadenitis in sheep and goats |
| title_full |
Corynebacterium pseudotuberculosis secreted antigen-induced specific gamma-interferon production by peripheral blood leukocytes: potential diagnostic marker for caseous lymphadenitis in sheep and goats |
| title_fullStr |
Corynebacterium pseudotuberculosis secreted antigen-induced specific gamma-interferon production by peripheral blood leukocytes: potential diagnostic marker for caseous lymphadenitis in sheep and goats |
| title_full_unstemmed |
Corynebacterium pseudotuberculosis secreted antigen-induced specific gamma-interferon production by peripheral blood leukocytes: potential diagnostic marker for caseous lymphadenitis in sheep and goats |
| title_sort |
Corynebacterium pseudotuberculosis secreted antigen-induced specific gamma-interferon production by peripheral blood leukocytes: potential diagnostic marker for caseous lymphadenitis in sheep and goats |
| author |
Rebouças, Miriam Flores |
| author_facet |
Rebouças, Miriam Flores Portela, Ricardo Wagner Dias Lima, Danielle Dantas Loureiro, Dan Bastos, Bruno Lopes Costa, Lília Ferreira de Moura Vale, Vera Lúcia Costa Miyoshi, Anderson Azevedo, Vasco Ariston de Carvalho Nascimento, Roberto José Meyer |
| author_role |
author |
| author2 |
Portela, Ricardo Wagner Dias Lima, Danielle Dantas Loureiro, Dan Bastos, Bruno Lopes Costa, Lília Ferreira de Moura Vale, Vera Lúcia Costa Miyoshi, Anderson Azevedo, Vasco Ariston de Carvalho Nascimento, Roberto José Meyer |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Rebouças, Miriam Flores Portela, Ricardo Wagner Dias Lima, Danielle Dantas Loureiro, Dan Bastos, Bruno Lopes Costa, Lília Ferreira de Moura Vale, Vera Lúcia Costa Miyoshi, Anderson Azevedo, Vasco Ariston de Carvalho Nascimento, Roberto José Meyer Rebouças, Miriam Flores Portela, Ricardo Wagner Dias Lima, Danielle Dantas Loureiro, Dan Bastos, Bruno Lopes Costa, Lília Ferreira de Moura Vale, Vera Lúcia Costa Miyoshi, Anderson Azevedo, Vasco Ariston de Carvalho Nascimento, Roberto José Meyer |
| dc.subject.por.fl_str_mv |
Caseous lymphadenitis goats sheep Corynebacterium pseudotuberculosis |
| topic |
Caseous lymphadenitis goats sheep Corynebacterium pseudotuberculosis |
| description |
p. 213–220 |
| publishDate |
2011 |
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2011-12-16T12:25:46Z |
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2011-12-16T12:25:46Z |
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2011 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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http://www.repositorio.ufba.br/ri/handle/ri/4873 |
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1040-6387 |
| dc.identifier.number.pt_BR.fl_str_mv |
v. 23 |
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1040-6387 v. 23 |
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