Mesenchymal stem cells in a polycaprolactone conduit enhance median-nerve regeneration, prevent decrease of creatine phosphokinase levels in muscle, and improve functional recovery in mice

Detalhes bibliográficos
Autor(a) principal: Oliveira, J. T.
Data de Publicação: 2010
Outros Autores: Almeida, F. M., Biancalana, A., Baptista, Abrahão Fontes, Tomaz, Marcelo Antonio, Melo, Paulo A., Martinez, A. M. B.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/13657
Resumo: Texto completo: acesso restrito. p.1295-1303
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spelling Oliveira, J. T.Almeida, F. M.Biancalana, A.Baptista, Abrahão FontesTomaz, Marcelo AntonioMelo, Paulo A.Martinez, A. M. B.Oliveira, J. T.Almeida, F. M.Biancalana, A.Baptista, Abrahão FontesTomaz, Marcelo AntonioMelo, Paulo A.Martinez, A. M. B.2013-11-16T12:00:54Z20100306-4522http://repositorio.ufba.br/ri/handle/ri/13657v. 170, n. 4Texto completo: acesso restrito. p.1295-1303Although the majority of peripheral-nerve regeneration studies are carried out on the sciatic nerve, lesions of the upper extremities are more common in humans and usually lead to significant physical disabilities. The present study was driven by the hypothesis that a combination of strategies, namely grafts of mesenchymal stem cells (MSC) and resorbable polycaprolactone (PCL) conduits would improve median-nerve regeneration after transection. Mouse median nerves were transected and sutured to PCL tubes that were filled with either green fluorescent protein (GFP+) MSC in DMEM or with DMEM alone. During the post-operative period, animals were tested weekly for flexor digitorum muscle function by means of the grasping test. After 8 weeks, the proximal and middle portions of the PCL tube and the regenerating nerves were harvested and processed for light and electron microscopy. The flexor digitorum muscle was weighed and subjected to biochemical analysis for creatine phosphokinase (CK) levels. Scanning electron microscopy of the PCL tube 8 weeks after implantation showed clear signs of wall disintegration. MSC-treated animals showed significantly larger numbers of myelinated and unmyelinated nerve fibers and blood vessels compared with DMEM-treated animals. The flexor digitorum muscle CK levels were significantly higher in the MSC-treated animals, but muscle weight values did not differ between the groups. Compared with the DMEM-treated group, MSC-treated animals showed, by the grasping test, improved functional performance throughout the period analyzed. Immunofluorescence for S-100 and GFP showed, in a few cases, double-labeled cells, suggesting that transplanted cells may occasionally transdifferentiate into Schwann cells. Our data demonstrate that the polycaprolactone conduit filled with MSC is capable of significantly improving the median-nerve regeneration after a traumatic lesion.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-07-04T17:13:39Z No. of bitstreams: 1 1-s2.0-S030645221001170X-main.pdf: 4140637 bytes, checksum: 3628235a3b61c5bffd2ec68d551dc638 (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2013-11-16T12:00:54Z (GMT) No. of bitstreams: 1 1-s2.0-S030645221001170X-main.pdf: 4140637 bytes, checksum: 3628235a3b61c5bffd2ec68d551dc638 (MD5)Made available in DSpace on 2013-11-16T12:00:54Z (GMT). No. of bitstreams: 1 1-s2.0-S030645221001170X-main.pdf: 4140637 bytes, checksum: 3628235a3b61c5bffd2ec68d551dc638 (MD5) Previous issue date: 2010http://dx.doi.org.ez10.periodicos.capes.gov.br/10.1016/j.neuroscience.2010.08.042reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBACell therapyMesenchymal cellsMedian nervePolycaprolactone conduitNerve regenerationGrasping testMesenchymal stem cells in a polycaprolactone conduit enhance median-nerve regeneration, prevent decrease of creatine phosphokinase levels in muscle, and improve functional recovery in miceNeuroscienceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINAL1-s2.0-S030645221001170X-main.pdf1-s2.0-S030645221001170X-main.pdfapplication/pdf4140637https://repositorio.ufba.br/bitstream/ri/13657/1/1-s2.0-S030645221001170X-main.pdf3628235a3b61c5bffd2ec68d551dc638MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/13657/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT1-s2.0-S030645221001170X-main.pdf.txt1-s2.0-S030645221001170X-main.pdf.txtExtracted texttext/plain43903https://repositorio.ufba.br/bitstream/ri/13657/3/1-s2.0-S030645221001170X-main.pdf.txte13a31947739ed44e2f7b40e56fc2a2cMD53ri/136572022-07-05 14:04:14.603oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:04:14Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Mesenchymal stem cells in a polycaprolactone conduit enhance median-nerve regeneration, prevent decrease of creatine phosphokinase levels in muscle, and improve functional recovery in mice
dc.title.alternative.pt_BR.fl_str_mv Neuroscience
title Mesenchymal stem cells in a polycaprolactone conduit enhance median-nerve regeneration, prevent decrease of creatine phosphokinase levels in muscle, and improve functional recovery in mice
spellingShingle Mesenchymal stem cells in a polycaprolactone conduit enhance median-nerve regeneration, prevent decrease of creatine phosphokinase levels in muscle, and improve functional recovery in mice
Oliveira, J. T.
Cell therapy
Mesenchymal cells
Median nerve
Polycaprolactone conduit
Nerve regeneration
Grasping test
title_short Mesenchymal stem cells in a polycaprolactone conduit enhance median-nerve regeneration, prevent decrease of creatine phosphokinase levels in muscle, and improve functional recovery in mice
title_full Mesenchymal stem cells in a polycaprolactone conduit enhance median-nerve regeneration, prevent decrease of creatine phosphokinase levels in muscle, and improve functional recovery in mice
title_fullStr Mesenchymal stem cells in a polycaprolactone conduit enhance median-nerve regeneration, prevent decrease of creatine phosphokinase levels in muscle, and improve functional recovery in mice
title_full_unstemmed Mesenchymal stem cells in a polycaprolactone conduit enhance median-nerve regeneration, prevent decrease of creatine phosphokinase levels in muscle, and improve functional recovery in mice
title_sort Mesenchymal stem cells in a polycaprolactone conduit enhance median-nerve regeneration, prevent decrease of creatine phosphokinase levels in muscle, and improve functional recovery in mice
author Oliveira, J. T.
author_facet Oliveira, J. T.
Almeida, F. M.
Biancalana, A.
Baptista, Abrahão Fontes
Tomaz, Marcelo Antonio
Melo, Paulo A.
Martinez, A. M. B.
author_role author
author2 Almeida, F. M.
Biancalana, A.
Baptista, Abrahão Fontes
Tomaz, Marcelo Antonio
Melo, Paulo A.
Martinez, A. M. B.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, J. T.
Almeida, F. M.
Biancalana, A.
Baptista, Abrahão Fontes
Tomaz, Marcelo Antonio
Melo, Paulo A.
Martinez, A. M. B.
Oliveira, J. T.
Almeida, F. M.
Biancalana, A.
Baptista, Abrahão Fontes
Tomaz, Marcelo Antonio
Melo, Paulo A.
Martinez, A. M. B.
dc.subject.por.fl_str_mv Cell therapy
Mesenchymal cells
Median nerve
Polycaprolactone conduit
Nerve regeneration
Grasping test
topic Cell therapy
Mesenchymal cells
Median nerve
Polycaprolactone conduit
Nerve regeneration
Grasping test
description Texto completo: acesso restrito. p.1295-1303
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2013-11-16T12:00:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/13657
dc.identifier.issn.none.fl_str_mv 0306-4522
dc.identifier.number.pt_BR.fl_str_mv v. 170, n. 4
identifier_str_mv 0306-4522
v. 170, n. 4
url http://repositorio.ufba.br/ri/handle/ri/13657
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.pt_BR.fl_str_mv http://dx.doi.org.ez10.periodicos.capes.gov.br/10.1016/j.neuroscience.2010.08.042
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