Bats carry pathogenic hepadnaviruses antigenically related to hepatitis B virus and capable of infecting human hepatocytes

Detalhes bibliográficos
Autor(a) principal: Drexler, Jan Felix
Data de Publicação: 2013
Outros Autores: Geipel, Andreas, König, Alexander, Corman, Victor Max, Riel, Debby van, Carneiro, Aroldo José Borges, Franke, Carlos Roberto
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/16380
Resumo: Texto completo: acesso restrito. p. 16151–16156
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spelling Drexler, Jan FelixGeipel, AndreasKönig, AlexanderCorman, Victor MaxRiel, Debby vanCarneiro, Aroldo José BorgesFranke, Carlos RobertoDrexler, Jan FelixGeipel, AndreasKönig, AlexanderCorman, Victor MaxRiel, Debby vanCarneiro, Aroldo José BorgesFranke, Carlos Roberto2014-10-10T15:08:08Z20131091-6490http://repositorio.ufba.br/ri/handle/ri/16380v. 110, n. 40Texto completo: acesso restrito. p. 16151–16156The hepatitis B virus (HBV), family Hepadnaviridae, is one of most relevant human pathogens. HBV origins are enigmatic, and no zoonotic reservoirs are known. Here, we screened 3,080 specimens from 54 bat species representing 11 bat families for hepadnaviral DNA. Ten specimens (0.3%) from Panama and Gabon yielded unique hepadnaviruses in coancestral relation to HBV. Full genome sequencing allowed classification as three putative orthohepadnavirus species based on genome lengths (3,149–3,377 nt), presence of middle HBV surface and X-protein genes, and sequence distance criteria. Hepatic tropism in bats was shown by quantitative PCR and in situ hybridization. Infected livers showed histopathologic changes compatible with hepatitis. Human hepatocytes transfected with all three bat viruses cross-reacted with sera against the HBV core protein, concordant with the phylogenetic relatedness of these hepadnaviruses and HBV. One virus from Uroderma bilobatum, the tent-making bat, cross-reacted with monoclonal antibodies against the HBV antigenicity determining S domain. Up to 18.4% of bat sera contained antibodies against bat hepadnaviruses. Infectious clones were generated to study all three viruses in detail. Hepatitis D virus particles pseudotyped with surface proteins of U. bilobatum HBV, but neither of the other two viruses could infect primary human and Tupaia belangeri hepatocytes. Hepatocyte infection occurred through the human HBV receptor sodium taurocholate cotransporting polypeptide but could not be neutralized by sera from vaccinated humans. Antihepadnaviral treatment using an approved reverse transcriptase inhibitor blocked replication of all bat hepadnaviruses. Our data suggest that bats may have been ancestral sources of primate hepadnaviruses. The observed zoonotic potential might affect concepts aimed at eradicating HBV.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-04-29T13:19:54Z No. of bitstreams: 1 10.1073pnas.1308049110.pdf: 2579959 bytes, checksum: 233110daea0d28682ff235572a9a6230 (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2014-10-10T15:08:08Z (GMT) No. of bitstreams: 1 10.1073pnas.1308049110.pdf: 2579959 bytes, checksum: 233110daea0d28682ff235572a9a6230 (MD5)Made available in DSpace on 2014-10-10T15:08:08Z (GMT). No. of bitstreams: 1 10.1073pnas.1308049110.pdf: 2579959 bytes, checksum: 233110daea0d28682ff235572a9a6230 (MD5) Previous issue date: 2013http://dx.doi.org/10.1073/pnas.1308049110reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAEvolutionZoonosisViromeMetagenomicsReverse geneticsBats carry pathogenic hepadnaviruses antigenically related to hepatitis B virus and capable of infecting human hepatocytesProceedings of the National Academy of Sciencesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINAL10.1073pnas.1308049110.pdf10.1073pnas.1308049110.pdfapplication/pdf2579959https://repositorio.ufba.br/bitstream/ri/16380/1/10.1073pnas.1308049110.pdf233110daea0d28682ff235572a9a6230MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/16380/2/license.txt0d4b811ef71182510d2015daa7c8a900MD52TEXT10.1073pnas.1308049110.pdf.txt10.1073pnas.1308049110.pdf.txtExtracted texttext/plain77528https://repositorio.ufba.br/bitstream/ri/16380/3/10.1073pnas.1308049110.pdf.txt2fbd73ff5c60d06a4972c55a76839eccMD53ri/163802022-07-05 14:03:01.854oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:01Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Bats carry pathogenic hepadnaviruses antigenically related to hepatitis B virus and capable of infecting human hepatocytes
dc.title.alternative.pt_BR.fl_str_mv Proceedings of the National Academy of Sciences
title Bats carry pathogenic hepadnaviruses antigenically related to hepatitis B virus and capable of infecting human hepatocytes
spellingShingle Bats carry pathogenic hepadnaviruses antigenically related to hepatitis B virus and capable of infecting human hepatocytes
Drexler, Jan Felix
Evolution
Zoonosis
Virome
Metagenomics
Reverse genetics
title_short Bats carry pathogenic hepadnaviruses antigenically related to hepatitis B virus and capable of infecting human hepatocytes
title_full Bats carry pathogenic hepadnaviruses antigenically related to hepatitis B virus and capable of infecting human hepatocytes
title_fullStr Bats carry pathogenic hepadnaviruses antigenically related to hepatitis B virus and capable of infecting human hepatocytes
title_full_unstemmed Bats carry pathogenic hepadnaviruses antigenically related to hepatitis B virus and capable of infecting human hepatocytes
title_sort Bats carry pathogenic hepadnaviruses antigenically related to hepatitis B virus and capable of infecting human hepatocytes
author Drexler, Jan Felix
author_facet Drexler, Jan Felix
Geipel, Andreas
König, Alexander
Corman, Victor Max
Riel, Debby van
Carneiro, Aroldo José Borges
Franke, Carlos Roberto
author_role author
author2 Geipel, Andreas
König, Alexander
Corman, Victor Max
Riel, Debby van
Carneiro, Aroldo José Borges
Franke, Carlos Roberto
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Drexler, Jan Felix
Geipel, Andreas
König, Alexander
Corman, Victor Max
Riel, Debby van
Carneiro, Aroldo José Borges
Franke, Carlos Roberto
Drexler, Jan Felix
Geipel, Andreas
König, Alexander
Corman, Victor Max
Riel, Debby van
Carneiro, Aroldo José Borges
Franke, Carlos Roberto
dc.subject.por.fl_str_mv Evolution
Zoonosis
Virome
Metagenomics
Reverse genetics
topic Evolution
Zoonosis
Virome
Metagenomics
Reverse genetics
description Texto completo: acesso restrito. p. 16151–16156
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-10-10T15:08:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/16380
dc.identifier.issn.none.fl_str_mv 1091-6490
dc.identifier.number.pt_BR.fl_str_mv v. 110, n. 40
identifier_str_mv 1091-6490
v. 110, n. 40
url http://repositorio.ufba.br/ri/handle/ri/16380
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1073/pnas.1308049110
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
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reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
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