Intramitochondrial crystalline inclusions in nonalcoholic steatohepatitis

Detalhes bibliográficos
Autor(a) principal: Caldwell, Stephen H.
Data de Publicação: 2009
Outros Autores: Freitas, Luiz Antonio R. de, Park, Sang H., Moreno, Maria Lucia Vieira, Redick, Jan A., Sisson, Barbee J., Patrie, James T., Cotrim, Helma Pinchemel, Argo, Curtis K., Al-Osaimi, Abdullah
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/13427
Resumo: Texto completo: acesso restrito. p. 1888-1895
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spelling Caldwell, Stephen H.Freitas, Luiz Antonio R. dePark, Sang H.Moreno, Maria Lucia VieiraRedick, Jan A.Sisson, Barbee J.Patrie, James T.Cotrim, Helma PinchemelArgo, Curtis K.Al-Osaimi, AbdullahCaldwell, Stephen H.Freitas, Luiz Antonio R. dePark, Sang H.Moreno, Maria Lucia VieiraRedick, Jan A.Sisson, Barbee J.Patrie, James T.Cotrim, Helma PinchemelArgo, Curtis K.Al-Osaimi, Abdullah2013-10-31T18:06:28Z20090270-9139http://repositorio.ufba.br/ri/handle/ri/13427v. 49, n. 6Texto completo: acesso restrito. p. 1888-1895Mitochondrial dysfunction is an important element in the pathogenesis of nonalcoholic steatohepatitis (NASH). Intramitochondrial crystals (IMCs) are a well-documented morphological abnormality seen on transmission electron microscopy in this disease. It has been suggested that IMCs consist of phospholipids, but their exact composition remain uncertain many years after their discovery. Micellar phase transitions of phospholipid bilayers is a well-known but little-studied phenomenon in living systems. Its presence in the mitochondria of NASH would offer significant insight into the disease with possible therapeutic implications. We postulated that intramitochondrial disturbances in NASH are sufficient to produce such transitions and that their detection in fresh biopsies would therefore be a dynamic process. To test this, we performed a blinded, prospective analysis of fresh liver biopsy samples immediately fixed under different conditions. Quantitative transmission electron microscopy morphometry, performed by systematically counting total mitochondria and IMCs within areas of uniform dimension, showed a stepwise decline in IMCs with cooler fixation temperature in each subject studied. Randomization testing (Monte Carlo resampling) confirmed that the detection of IMCs was strongly dependent on fixation temperature (P < 0.0001). Conclusion: These results indicate that the intramitochondrial crystals characteristic of NASH are highly dynamic and unstable structures. The findings offer the strongest support yet for their origin in micellar phase transitions. We speculate that such transitions result from microenvironmental changes within the mitochondria and carry therapeutic implications, especially in regard to dietary manipulations of mitochondrial lipid composition. (HEPATOLOGY 2009.)Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-10-08T15:04:48Z No. of bitstreams: 1 10.1002-hep.22851.pdf: 713356 bytes, checksum: 0e52c591d472e0208fbc7f58ad3670c9 (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2013-10-31T18:06:28Z (GMT) No. of bitstreams: 1 10.1002-hep.22851.pdf: 713356 bytes, checksum: 0e52c591d472e0208fbc7f58ad3670c9 (MD5)Made available in DSpace on 2013-10-31T18:06:28Z (GMT). No. of bitstreams: 1 10.1002-hep.22851.pdf: 713356 bytes, checksum: 0e52c591d472e0208fbc7f58ad3670c9 (MD5) Previous issue date: 2009http://dx.doi.org/10.1002/hep.22851reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAIntramitochondrial crystalline inclusions in nonalcoholic steatohepatitisHepatologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINAL10.1002-hep.22851.pdf10.1002-hep.22851.pdfapplication/pdf713356https://repositorio.ufba.br/bitstream/ri/13427/1/10.1002-hep.22851.pdf0e52c591d472e0208fbc7f58ad3670c9MD51LICENSElicense.txtlicense.txttext/plain1365https://repositorio.ufba.br/bitstream/ri/13427/2/license.txt5371a150bdc863f78dcf39281543bd86MD52TEXT10.1002-hep.22851.pdf.txt10.1002-hep.22851.pdf.txtExtracted texttext/plain34970https://repositorio.ufba.br/bitstream/ri/13427/3/10.1002-hep.22851.pdf.txtd6e67549064faa9692c00db23f7d59c8MD53ri/134272022-07-05 14:03:43.371oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:43Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Intramitochondrial crystalline inclusions in nonalcoholic steatohepatitis
dc.title.alternative.pt_BR.fl_str_mv Hepatology
title Intramitochondrial crystalline inclusions in nonalcoholic steatohepatitis
spellingShingle Intramitochondrial crystalline inclusions in nonalcoholic steatohepatitis
Caldwell, Stephen H.
title_short Intramitochondrial crystalline inclusions in nonalcoholic steatohepatitis
title_full Intramitochondrial crystalline inclusions in nonalcoholic steatohepatitis
title_fullStr Intramitochondrial crystalline inclusions in nonalcoholic steatohepatitis
title_full_unstemmed Intramitochondrial crystalline inclusions in nonalcoholic steatohepatitis
title_sort Intramitochondrial crystalline inclusions in nonalcoholic steatohepatitis
author Caldwell, Stephen H.
author_facet Caldwell, Stephen H.
Freitas, Luiz Antonio R. de
Park, Sang H.
Moreno, Maria Lucia Vieira
Redick, Jan A.
Sisson, Barbee J.
Patrie, James T.
Cotrim, Helma Pinchemel
Argo, Curtis K.
Al-Osaimi, Abdullah
author_role author
author2 Freitas, Luiz Antonio R. de
Park, Sang H.
Moreno, Maria Lucia Vieira
Redick, Jan A.
Sisson, Barbee J.
Patrie, James T.
Cotrim, Helma Pinchemel
Argo, Curtis K.
Al-Osaimi, Abdullah
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Caldwell, Stephen H.
Freitas, Luiz Antonio R. de
Park, Sang H.
Moreno, Maria Lucia Vieira
Redick, Jan A.
Sisson, Barbee J.
Patrie, James T.
Cotrim, Helma Pinchemel
Argo, Curtis K.
Al-Osaimi, Abdullah
Caldwell, Stephen H.
Freitas, Luiz Antonio R. de
Park, Sang H.
Moreno, Maria Lucia Vieira
Redick, Jan A.
Sisson, Barbee J.
Patrie, James T.
Cotrim, Helma Pinchemel
Argo, Curtis K.
Al-Osaimi, Abdullah
description Texto completo: acesso restrito. p. 1888-1895
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2013-10-31T18:06:28Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/13427
dc.identifier.issn.none.fl_str_mv 0270-9139
dc.identifier.number.pt_BR.fl_str_mv v. 49, n. 6
identifier_str_mv 0270-9139
v. 49, n. 6
url http://repositorio.ufba.br/ri/handle/ri/13427
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1002/hep.22851
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