Proliferation of human bone marrow stem cells cultured on alfa-tricalcium phosphate cement and titanium discs

• Autor(a) principal: Paris, Marcel Fasolo de
• Data de Publicação: 2014
• Outros Autores: Baraldi, Carlos Eduardo, Viezzer, Christian, Machado, Denise Cantarelli, Oliveira, Marília Gerhardt de
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Repositório Institucional da UFBA
• Texto Completo: http://repositorio.ufba.br/ri/handle/ri/23134
• topic: Células-tronco.; Fosfatos de cálcio.; Titânio.

Abstract Objectives: to evaluate human bone marrow stem cells (HBMSC) cultured on α -tricalcium phosphate cement (α -TCP) and titanium (Ti) discs, as well as the osteoconductive properties of these biomaterials. Methods: discs of α -TCP and commercially pure Ti were manufactured and placed on polyethylene plates. Cells were cultured according to the following: group I – control (no discs); group II – α-TCP discs; group III – Ti discs. Propidium iodide staining was used to assess cell proliferation. Cells cultures were analyzed at 3, 7, 14 and 21 days. Results: Proliferation occurred in both biomaterials. At 3 days, greater cell proliferation was seen in group II than group III (p=0.01). Differences were not significant on the other time periods. Morphologic analysis showed circular morphology in group I, compatible with osteogenic cell lineage. Conclusions: both biomaterials were osteoconductive but not osteoinductive. This did not affect the initial stages of osteogenesis. Keywords: Stem cells. Calcium phosphates. Titanium.