Identification and characterization of a class III chitin synthase gene of Moniliophthora perniciosa, the fungus that causes witches’ broom disease of cacao

Detalhes bibliográficos
Autor(a) principal: Souza, Catiane S.
Data de Publicação: 2009
Outros Autores: Oliveira, Bruno M., Costa, Gustavo G. L., Schriefer, Nicolaus Albert Borges, Schnadelbach, Alessandra Selbach, Uetanabaro, Ana Paula T., Pirovani, Carlos P.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/13273
Resumo: Texto completo: acesso restrito. p.431-440
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spelling Souza, Catiane S.Oliveira, Bruno M.Costa, Gustavo G. L.Schriefer, Nicolaus Albert BorgesSchnadelbach, Alessandra SelbachUetanabaro, Ana Paula T.Pirovani, Carlos P.Souza, Catiane S.Oliveira, Bruno M.Costa, Gustavo G. L.Schriefer, Nicolaus Albert BorgesSchnadelbach, Alessandra SelbachUetanabaro, Ana Paula T.Pirovani, Carlos P.2013-10-15T18:30:06Z20091225-8873http://www.repositorio.ufba.br/ri/handle/ri/13273v. 47, n. 4Texto completo: acesso restrito. p.431-440Chitin synthase (CHS) is a glucosyltransferase that converts UDP-N-acetylglucosamine into chitin, one of the main components of fungal cell wall. Class III chitin synthases act directly in the formation of the cell wall. They catalyze the conversion of the immediate precursor of chitin and are responsible for the majority of chitin synthesis in fungi. As such, they are highly specific molecular targets for drugs that can inhibit the growth and development of fungal pathogens. In this work, we have identified and characterized a chitin synthase gene of Moniliophthora perniciosa (Mopchs) by primer walking. The complete gene sequence is 3,443 bp, interrupted by 13 small introns, and comprises a cDNA with an ORF with 2,739 bp, whose terminal region was experimentally determined, encoding a protein with 913 aa that harbors all the motifs and domains typically found in class III chitin synthases. This is the first report on the characterization of a chitin synthase gene, its mature transcription product, and its putative protein in basidioma and secondary mycelium stages of M. perniciosa, a basidiomycotan fungus that causes witches’ broom disease of cacao.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-10-01T14:11:27Z No. of bitstreams: 1 art%3A10.1007%2Fs12275-008-0166-3.pdf: 336925 bytes, checksum: 6cd3458aea869797d5ba7a9cc26e5653 (MD5)Approved for entry into archive by Alda Lima da Silva(sivalda@ufba.br) on 2013-10-15T18:30:06Z (GMT) No. of bitstreams: 1 art%3A10.1007%2Fs12275-008-0166-3.pdf: 336925 bytes, checksum: 6cd3458aea869797d5ba7a9cc26e5653 (MD5)Made available in DSpace on 2013-10-15T18:30:06Z (GMT). No. of bitstreams: 1 art%3A10.1007%2Fs12275-008-0166-3.pdf: 336925 bytes, checksum: 6cd3458aea869797d5ba7a9cc26e5653 (MD5) Previous issue date: 2009http://dx.doi.org/10.1007/s12275-008-0166-3reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBACell wallChitinChitin synthaseWitches’ broom diseaseCacaoIdentification and characterization of a class III chitin synthase gene of Moniliophthora perniciosa, the fungus that causes witches’ broom disease of cacaoJournal of Microbiologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessLICENSElicense.txtlicense.txttext/plain1365https://repositorio.ufba.br/bitstream/ri/13273/2/license.txt5371a150bdc863f78dcf39281543bd86MD52ORIGINALart%3A10.1007%2Fs12275-008-0166-3.pdfart%3A10.1007%2Fs12275-008-0166-3.pdfapplication/pdf336925https://repositorio.ufba.br/bitstream/ri/13273/1/art%253A10.1007%252Fs12275-008-0166-3.pdf6cd3458aea869797d5ba7a9cc26e5653MD51TEXTart%3A10.1007%2Fs12275-008-0166-3.pdf.txtart%3A10.1007%2Fs12275-008-0166-3.pdf.txtExtracted texttext/plain43476https://repositorio.ufba.br/bitstream/ri/13273/3/art%253A10.1007%252Fs12275-008-0166-3.pdf.txt0247d22fac8b3bc5eaa59fc3baebb42dMD53ri/132732022-07-05 14:02:52.162oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:02:52Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Identification and characterization of a class III chitin synthase gene of Moniliophthora perniciosa, the fungus that causes witches’ broom disease of cacao
dc.title.alternative.pt_BR.fl_str_mv Journal of Microbiology
title Identification and characterization of a class III chitin synthase gene of Moniliophthora perniciosa, the fungus that causes witches’ broom disease of cacao
spellingShingle Identification and characterization of a class III chitin synthase gene of Moniliophthora perniciosa, the fungus that causes witches’ broom disease of cacao
Souza, Catiane S.
Cell wall
Chitin
Chitin synthase
Witches’ broom disease
Cacao
title_short Identification and characterization of a class III chitin synthase gene of Moniliophthora perniciosa, the fungus that causes witches’ broom disease of cacao
title_full Identification and characterization of a class III chitin synthase gene of Moniliophthora perniciosa, the fungus that causes witches’ broom disease of cacao
title_fullStr Identification and characterization of a class III chitin synthase gene of Moniliophthora perniciosa, the fungus that causes witches’ broom disease of cacao
title_full_unstemmed Identification and characterization of a class III chitin synthase gene of Moniliophthora perniciosa, the fungus that causes witches’ broom disease of cacao
title_sort Identification and characterization of a class III chitin synthase gene of Moniliophthora perniciosa, the fungus that causes witches’ broom disease of cacao
author Souza, Catiane S.
author_facet Souza, Catiane S.
Oliveira, Bruno M.
Costa, Gustavo G. L.
Schriefer, Nicolaus Albert Borges
Schnadelbach, Alessandra Selbach
Uetanabaro, Ana Paula T.
Pirovani, Carlos P.
author_role author
author2 Oliveira, Bruno M.
Costa, Gustavo G. L.
Schriefer, Nicolaus Albert Borges
Schnadelbach, Alessandra Selbach
Uetanabaro, Ana Paula T.
Pirovani, Carlos P.
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Souza, Catiane S.
Oliveira, Bruno M.
Costa, Gustavo G. L.
Schriefer, Nicolaus Albert Borges
Schnadelbach, Alessandra Selbach
Uetanabaro, Ana Paula T.
Pirovani, Carlos P.
Souza, Catiane S.
Oliveira, Bruno M.
Costa, Gustavo G. L.
Schriefer, Nicolaus Albert Borges
Schnadelbach, Alessandra Selbach
Uetanabaro, Ana Paula T.
Pirovani, Carlos P.
dc.subject.por.fl_str_mv Cell wall
Chitin
Chitin synthase
Witches’ broom disease
Cacao
topic Cell wall
Chitin
Chitin synthase
Witches’ broom disease
Cacao
description Texto completo: acesso restrito. p.431-440
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2013-10-15T18:30:06Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/13273
dc.identifier.issn.none.fl_str_mv 1225-8873
dc.identifier.number.pt_BR.fl_str_mv v. 47, n. 4
identifier_str_mv 1225-8873
v. 47, n. 4
url http://www.repositorio.ufba.br/ri/handle/ri/13273
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1007/s12275-008-0166-3
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