Structural basis for selective inhibition of purine nucleoside phosphorylase from Schistosoma mansoni: Kinetic and structural studies

Detalhes bibliográficos
Autor(a) principal: Castilho, Marcelo Santos
Data de Publicação: 2010
Outros Autores: Postigo, Matheus P., Pereira, Humberto M., Oliva, Glaucius, Andricopulo, Adriano D.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/5984
Resumo: Trabalho completo: acesso restrito, p. 1421–1427
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spelling Castilho, Marcelo SantosPostigo, Matheus P.Pereira, Humberto M.Oliva, GlauciusAndricopulo, Adriano D.Castilho, Marcelo SantosPostigo, Matheus P.Pereira, Humberto M.Oliva, GlauciusAndricopulo, Adriano D.2012-05-29T16:52:18Z20100968-0896http://www.repositorio.ufba.br/ri/handle/ri/5984v. 18, n. 4Trabalho completo: acesso restrito, p. 1421–1427Selectivity plays a crucial role in the design of enzyme inhibitors as novel antiparasitic agents, particularly in cases where the target enzyme is also present in the human host. Purine nucleoside phosphorylase from Schistosoma mansoni (SmPNP) is an attractive target for the discovery of potential antischistosomal agents. In the present work, kinetic studies were carried out in order to determine the inhibitory potency, mode of action and enzyme selectivity of a series of inhibitors of SmPNP. In addition, crystallographic studies provided important structural insights for rational inhibitor design, revealing consistent structural differences in the binding mode of the inhibitors in the active sites of the SmPNP and human PNP (HsPNP) structures. The molecular information gathered in this work should be useful for future medicinal chemistry efforts in the design of new inhibitors of SmPNP having increased affinity and selectivity.Submitted by Bruna Lessa (lessbruna@gmail.com) on 2012-05-29T16:52:18Z No. of bitstreams: 1 (39)1-s2.0-S096808961000043X-main.pdf: 998932 bytes, checksum: 90bd5773640cb0d48d8c9ddf633bc2d8 (MD5)Made available in DSpace on 2012-05-29T16:52:18Z (GMT). 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dc.title.pt_BR.fl_str_mv Structural basis for selective inhibition of purine nucleoside phosphorylase from Schistosoma mansoni: Kinetic and structural studies
dc.title.alternative.pt_BR.fl_str_mv Bioorganic & Medicinal Chemistry
title Structural basis for selective inhibition of purine nucleoside phosphorylase from Schistosoma mansoni: Kinetic and structural studies
spellingShingle Structural basis for selective inhibition of purine nucleoside phosphorylase from Schistosoma mansoni: Kinetic and structural studies
Castilho, Marcelo Santos
Neglected tropical diseases
Schistosomiasis
Enzyme inhibition
Selectivity
title_short Structural basis for selective inhibition of purine nucleoside phosphorylase from Schistosoma mansoni: Kinetic and structural studies
title_full Structural basis for selective inhibition of purine nucleoside phosphorylase from Schistosoma mansoni: Kinetic and structural studies
title_fullStr Structural basis for selective inhibition of purine nucleoside phosphorylase from Schistosoma mansoni: Kinetic and structural studies
title_full_unstemmed Structural basis for selective inhibition of purine nucleoside phosphorylase from Schistosoma mansoni: Kinetic and structural studies
title_sort Structural basis for selective inhibition of purine nucleoside phosphorylase from Schistosoma mansoni: Kinetic and structural studies
author Castilho, Marcelo Santos
author_facet Castilho, Marcelo Santos
Postigo, Matheus P.
Pereira, Humberto M.
Oliva, Glaucius
Andricopulo, Adriano D.
author_role author
author2 Postigo, Matheus P.
Pereira, Humberto M.
Oliva, Glaucius
Andricopulo, Adriano D.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Castilho, Marcelo Santos
Postigo, Matheus P.
Pereira, Humberto M.
Oliva, Glaucius
Andricopulo, Adriano D.
Castilho, Marcelo Santos
Postigo, Matheus P.
Pereira, Humberto M.
Oliva, Glaucius
Andricopulo, Adriano D.
dc.subject.por.fl_str_mv Neglected tropical diseases
Schistosomiasis
Enzyme inhibition
Selectivity
topic Neglected tropical diseases
Schistosomiasis
Enzyme inhibition
Selectivity
description Trabalho completo: acesso restrito, p. 1421–1427
publishDate 2010
dc.date.issued.fl_str_mv 2010
dc.date.accessioned.fl_str_mv 2012-05-29T16:52:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/5984
dc.identifier.issn.none.fl_str_mv 0968-0896
dc.identifier.number.pt_BR.fl_str_mv v. 18, n. 4
identifier_str_mv 0968-0896
v. 18, n. 4
url http://www.repositorio.ufba.br/ri/handle/ri/5984
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.bmc.2010.01.022
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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