HTLV-1 infection in blood donors from the Western Brazilian Amazon region: seroprevalence and molecular study of viral isolates
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2008 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/13601 |
Resumo: | Texto completo: acesso restrito. p. 1966-1971 |
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Miranda, Aline Cristina MotaAraújo, Sérgio PereiraDias, Juarez PereiraColin, Denise DuizitKashima, SimoneCovas, Dimas TadeuTavares-Neto, JoséCastro, Bernardo GalvãoAlcântara, Luiz Carlos JúniorMiranda, Aline Cristina MotaAraújo, Sérgio PereiraDias, Juarez PereiraColin, Denise DuizitKashima, SimoneCovas, Dimas TadeuTavares-Neto, JoséCastro, Bernardo GalvãoAlcântara, Luiz Carlos Júnior2013-11-08T18:34:24Z20080146-6615http://repositorio.ufba.br/ri/handle/ri/13601v. 80, n. 11Texto completo: acesso restrito. p. 1966-1971To determine the seroprevalence of HTLV-1 in Brazil, and to review the virus molecular epidemiology in this Amazon population (Rio Branco-Acre), 219 blood donors were screened for HTLV-1. Only one case of infection (0.46% seroprevalence) was detected during July 2004 screening at the Acre Hospital Foundation (FUNDACRE). Neighbor-joining and Maximum Likelihood phylogenetic analyses of two (n = 2) complete LTR region sequences were performed with the PAUP* software. Since the HTLV-1 envelope surface (gp46) and transmembrane (gp21) glycoproteins are important for virus fitness, three envelope glycoproteins sequences (n = 3) were analyzed using the Prosite tool to determinate potential protein sites. Phylogenetic analysis demonstrated that the new isolate described in this study, and the unpublished LTR strain described in a previous report belong to the Transcontinental subgroup of the Cosmopolitan subtype, inside the Latin American cluster. A similar result was obtained when submitting, to the Automated Genotyping System, three LTR partial sequences from a previous study of the seroprevalence of HTLV-1 in the same Amazon population. In all analyzed env sequences, the potential protein site was found: two PKC phosphorylation sites at amino acid (aa) positions 310–312 and 342–344, one CK2 phosphorylation site at 194–197aa, three N-glycosylation sites at 222–225aa, 244–247aa and 272–275aa, and a single N-myristylation site at 327–338aa. In conclusion, potential protein sites described in HTLV-1 gp46 and gp21 confirm the presence of conserved sites in the HTLV-1 envelope proteins, likewise phylogenetic analysis suggests a possible recent introduction of the virus into North Brazil.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-11-07T13:41:49Z No. of bitstreams: 1 21300_ftp.pdf: 115572 bytes, checksum: 4e50eac27e29a127aa4ac829645eb933 (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2013-11-08T18:34:24Z (GMT) No. of bitstreams: 1 21300_ftp.pdf: 115572 bytes, checksum: 4e50eac27e29a127aa4ac829645eb933 (MD5)Made available in DSpace on 2013-11-08T18:34:24Z (GMT). No. of bitstreams: 1 21300_ftp.pdf: 115572 bytes, checksum: 4e50eac27e29a127aa4ac829645eb933 (MD5) Previous issue date: 2009http://dx.doi.org/10.1002/jmv.21300reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBALTR regionGlycoproteinsPhylogenetic analysisProtein sitesHTLV-1 infection in blood donors from the Western Brazilian Amazon region: seroprevalence and molecular study of viral isolatesJournal of Medical Virologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINAL21300_ftp.pdf21300_ftp.pdfapplication/pdf115572https://repositorio.ufba.br/bitstream/ri/13601/1/21300_ftp.pdf4e50eac27e29a127aa4ac829645eb933MD51LICENSElicense.txtlicense.txttext/plain1383https://repositorio.ufba.br/bitstream/ri/13601/2/license.txt05eca2f01d0b3307819d0369dab18a34MD52TEXT21300_ftp.pdf.txt21300_ftp.pdf.txtExtracted texttext/plain26882https://repositorio.ufba.br/bitstream/ri/13601/3/21300_ftp.pdf.txt961946bfeb145c5092400c2ea24bf2feMD53ri/136012022-10-22 18:19:51.982oai:repositorio.ufba.br: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ório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-10-22T21:19:51Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
HTLV-1 infection in blood donors from the Western Brazilian Amazon region: seroprevalence and molecular study of viral isolates |
| dc.title.alternative.pt_BR.fl_str_mv |
Journal of Medical Virology |
| title |
HTLV-1 infection in blood donors from the Western Brazilian Amazon region: seroprevalence and molecular study of viral isolates |
| spellingShingle |
HTLV-1 infection in blood donors from the Western Brazilian Amazon region: seroprevalence and molecular study of viral isolates Miranda, Aline Cristina Mota LTR region Glycoproteins Phylogenetic analysis Protein sites |
| title_short |
HTLV-1 infection in blood donors from the Western Brazilian Amazon region: seroprevalence and molecular study of viral isolates |
| title_full |
HTLV-1 infection in blood donors from the Western Brazilian Amazon region: seroprevalence and molecular study of viral isolates |
| title_fullStr |
HTLV-1 infection in blood donors from the Western Brazilian Amazon region: seroprevalence and molecular study of viral isolates |
| title_full_unstemmed |
HTLV-1 infection in blood donors from the Western Brazilian Amazon region: seroprevalence and molecular study of viral isolates |
| title_sort |
HTLV-1 infection in blood donors from the Western Brazilian Amazon region: seroprevalence and molecular study of viral isolates |
| author |
Miranda, Aline Cristina Mota |
| author_facet |
Miranda, Aline Cristina Mota Araújo, Sérgio Pereira Dias, Juarez Pereira Colin, Denise Duizit Kashima, Simone Covas, Dimas Tadeu Tavares-Neto, José Castro, Bernardo Galvão Alcântara, Luiz Carlos Júnior |
| author_role |
author |
| author2 |
Araújo, Sérgio Pereira Dias, Juarez Pereira Colin, Denise Duizit Kashima, Simone Covas, Dimas Tadeu Tavares-Neto, José Castro, Bernardo Galvão Alcântara, Luiz Carlos Júnior |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Miranda, Aline Cristina Mota Araújo, Sérgio Pereira Dias, Juarez Pereira Colin, Denise Duizit Kashima, Simone Covas, Dimas Tadeu Tavares-Neto, José Castro, Bernardo Galvão Alcântara, Luiz Carlos Júnior Miranda, Aline Cristina Mota Araújo, Sérgio Pereira Dias, Juarez Pereira Colin, Denise Duizit Kashima, Simone Covas, Dimas Tadeu Tavares-Neto, José Castro, Bernardo Galvão Alcântara, Luiz Carlos Júnior |
| dc.subject.por.fl_str_mv |
LTR region Glycoproteins Phylogenetic analysis Protein sites |
| topic |
LTR region Glycoproteins Phylogenetic analysis Protein sites |
| description |
Texto completo: acesso restrito. p. 1966-1971 |
| publishDate |
2008 |
| dc.date.issued.fl_str_mv |
2008 |
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2013-11-08T18:34:24Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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http://repositorio.ufba.br/ri/handle/ri/13601 |
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0146-6615 |
| dc.identifier.number.pt_BR.fl_str_mv |
v. 80, n. 11 |
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0146-6615 v. 80, n. 11 |
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http://repositorio.ufba.br/ri/handle/ri/13601 |
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http://dx.doi.org/10.1002/jmv.21300 |
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