TNF-alpha and IL-8: Serum levels and gene polymorphisms (ÿ308G>A and ÿ251A>T) are associated with classical biomarkers and medical history in children with sickle cell anemia
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2011 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/5422 |
Resumo: | texto completo: acesso restrito. p. 312–317. |
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Lyra, Isa MenezesMoura Neto, José Pereira deReis, Mitermayer Galvão dosBarbosa, Cynara GomesGonçalves, Marilda de SouzaCerqueira, Bruno Antonio VelosoCouto, Fábio DavidCajado, C.Santos, Wendell Vilas BoasDorea, M. J.Lyra, Isa MenezesMoura Neto, José Pereira deReis, Mitermayer Galvão dosBarbosa, Cynara GomesGonçalves, Marilda de SouzaCerqueira, Bruno Antonio VelosoCouto, Fábio DavidCajado, C.Santos, Wendell Vilas BoasDorea, M. J.2012-02-23T17:08:28Z20111096-0023http://www.repositorio.ufba.br/ri/handle/ri/5422v. 56.texto completo: acesso restrito. p. 312–317.Sickle cell anemia (SCA) is a disorder characterized by a heterogeneous clinical outcome. In the present study, we investigated the associations between Tumor Necrosis Factor-alpha (TNF-alpha) ÿ308G>A and Interleukin 8 (IL-8) ÿ251A>T gene polymorphisms, medical history and classical biomarkers in children with steady-state SCA. In total, 210 SCA patients aged 2–21 years and 200 healthy controls were studied. Gene polymorphisms, betaS-globin haplotypes and a 3.7-kb deletion in alpha2-thalassemia (a2-thal3.7 kb) were investigated by PCR/RFLP analysis, and cytokine levels were determined by ELISA. Splenomegaly (p = .032) was more prevalent among children younger than 5 years of age. The A allele of the TNF-alpha ÿ308G>A gene polymorphism and the presence of a2-thal3.7 kb were associated with an increase risk of splenic sequestration events (p = .001; p = .046), while the T allele of the IL-8 ÿ251A>T gene polymor-phism was considered to be a protective factor for splenomegaly events (p = .032). Moreover, the A allele of the TNF-alpha ÿ308G>A gene polymorphism was associated with high TNF-alpha levels (p = .021), and the hemoglobin F and hemoglobin S haplotypes were correlated with serum levels of IL-8. The logistic regression analysis showed significant effects of the TNF-alpha and IL-8 gene polymorphisms, betaS-globin gene haplotypes and a2-thal3.7 kb on the occurrence of splenic sequestration events. Our study empha-sizes that the identification of new genetic and immunological biomarkers and their associations with classical markers is an important strategy to elucidate the underlying causes of different SCA phenotypes and their effects on patient outcome.Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2012-02-23T17:08:28Z No. of bitstreams: 1 __pdn.sciencedirect.com_....0-S1043466611002201-main.pdf: 165270 bytes, checksum: 0e51b46fddf119fd4edc9fc0857708a9 (MD5)Made available in DSpace on 2012-02-23T17:08:28Z (GMT). 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| dc.title.pt_BR.fl_str_mv |
TNF-alpha and IL-8: Serum levels and gene polymorphisms (ÿ308G>A and ÿ251A>T) are associated with classical biomarkers and medical history in children with sickle cell anemia |
| dc.title.alternative.pt_BR.fl_str_mv |
Cytokine |
| title |
TNF-alpha and IL-8: Serum levels and gene polymorphisms (ÿ308G>A and ÿ251A>T) are associated with classical biomarkers and medical history in children with sickle cell anemia |
| spellingShingle |
TNF-alpha and IL-8: Serum levels and gene polymorphisms (ÿ308G>A and ÿ251A>T) are associated with classical biomarkers and medical history in children with sickle cell anemia Lyra, Isa Menezes TNF-alpha IL-8 Sickle cell anemia betaS-globin Gene haplotypes alpha2-Thalassemia |
| title_short |
TNF-alpha and IL-8: Serum levels and gene polymorphisms (ÿ308G>A and ÿ251A>T) are associated with classical biomarkers and medical history in children with sickle cell anemia |
| title_full |
TNF-alpha and IL-8: Serum levels and gene polymorphisms (ÿ308G>A and ÿ251A>T) are associated with classical biomarkers and medical history in children with sickle cell anemia |
| title_fullStr |
TNF-alpha and IL-8: Serum levels and gene polymorphisms (ÿ308G>A and ÿ251A>T) are associated with classical biomarkers and medical history in children with sickle cell anemia |
| title_full_unstemmed |
TNF-alpha and IL-8: Serum levels and gene polymorphisms (ÿ308G>A and ÿ251A>T) are associated with classical biomarkers and medical history in children with sickle cell anemia |
| title_sort |
TNF-alpha and IL-8: Serum levels and gene polymorphisms (ÿ308G>A and ÿ251A>T) are associated with classical biomarkers and medical history in children with sickle cell anemia |
| author |
Lyra, Isa Menezes |
| author_facet |
Lyra, Isa Menezes Moura Neto, José Pereira de Reis, Mitermayer Galvão dos Barbosa, Cynara Gomes Gonçalves, Marilda de Souza Cerqueira, Bruno Antonio Veloso Couto, Fábio David Cajado, C. Santos, Wendell Vilas Boas Dorea, M. J. |
| author_role |
author |
| author2 |
Moura Neto, José Pereira de Reis, Mitermayer Galvão dos Barbosa, Cynara Gomes Gonçalves, Marilda de Souza Cerqueira, Bruno Antonio Veloso Couto, Fábio David Cajado, C. Santos, Wendell Vilas Boas Dorea, M. J. |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Lyra, Isa Menezes Moura Neto, José Pereira de Reis, Mitermayer Galvão dos Barbosa, Cynara Gomes Gonçalves, Marilda de Souza Cerqueira, Bruno Antonio Veloso Couto, Fábio David Cajado, C. Santos, Wendell Vilas Boas Dorea, M. J. Lyra, Isa Menezes Moura Neto, José Pereira de Reis, Mitermayer Galvão dos Barbosa, Cynara Gomes Gonçalves, Marilda de Souza Cerqueira, Bruno Antonio Veloso Couto, Fábio David Cajado, C. Santos, Wendell Vilas Boas Dorea, M. J. |
| dc.subject.por.fl_str_mv |
TNF-alpha IL-8 Sickle cell anemia betaS-globin Gene haplotypes alpha2-Thalassemia |
| topic |
TNF-alpha IL-8 Sickle cell anemia betaS-globin Gene haplotypes alpha2-Thalassemia |
| description |
texto completo: acesso restrito. p. 312–317. |
| publishDate |
2011 |
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2011 |
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2012-02-23T17:08:28Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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http://www.repositorio.ufba.br/ri/handle/ri/5422 |
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1096-0023 |
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v. 56. |
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1096-0023 v. 56. |
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http://www.repositorio.ufba.br/ri/handle/ri/5422 |
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eng |
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eng |
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openAccess |
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doi:10.1016/j.cyto.2011.07.002 |
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reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
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