Use of multiepitope polyproteins in serodiagnosis of active tuberculosis

Detalhes bibliográficos
Autor(a) principal: Houghton, Raymond L.
Data de Publicação: 2002
Outros Autores: Lodes, Michael J., Dillon, Davin C., Reynolds, Lisa D., Day, Craig H., Sampaio, Diana P., Badaró, Roberto José da Silva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/7466
Resumo: Texto completo: acesso restrito. p. 883-891
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spelling Houghton, Raymond L.Lodes, Michael J.Dillon, Davin C.Reynolds, Lisa D.Day, Craig H.Sampaio, Diana P.Badaró, Roberto José da SilvaHoughton, Raymond L.Lodes, Michael J.Dillon, Davin C.Reynolds, Lisa D.Day, Craig H.Sampaio, Diana P.Badaró, Roberto José da Silva2012-12-12T17:17:52Z2002-071071-412Xhttp://www.repositorio.ufba.br/ri/handle/ri/7466v. 9, n. 4Texto completo: acesso restrito. p. 883-891Screening of genomic expression libraries from Mycobacterium tuberculosis with sera from tuberculosis (TB) patients or rabbit antiserum to M. tuberculosis led to the identification of novel antigens capable of detecting specific antibodies to M. tuberculosis. Three antigens, Mtb11 (also known as CFP-10), Mtb8, and Mtb48, were tested together with the previously reported 38-kDa protein, in an enzyme-linked immunosorbent assay (ELISA) to detect antibodies in TB patients. These four proteins were also produced as a genetically fused polyprotein, which was tested with two additional antigens, DPEP (also known as MPT32) and Mtb81. Sera from individuals with pulmonary and extrapulmonary TB, human immunodeficiency virus (HIV)-TB coinfections, and purified protein derivative (PPD)-positive and PPD-negative status with no evidence of disease were tested. In samples from HIV-negative individuals, the ELISA detected antibodies in >80% of smear-positive individuals and >60% smear-negative individuals, with a specificity of ∼98%. For this group, smears detected 81.6% but a combination of smear and ELISA had a sensitivity of ∼93%. The antigen combination detected a significant number of HIV-TB coinfections as well as antibodies in patients with extrapulmonary infections. Improved reactivity in the HIV-TB group was observed by including the antigen Mtb81 that was identified by proteomics. The data indicate that the use of multiple antigens, some of which are in a single polyprotein, can be used to facilitate the development of a highly sensitive test for M. tuberculosis antibody detection.Submitted by Suelen Reis (suelen_suzane@hotmail.com) on 2012-12-12T17:17:52Z No. of bitstreams: 1 883.full.pdf: 457233 bytes, checksum: 7db64d35a444401b1d436a051966ef0d (MD5)Made available in DSpace on 2012-12-12T17:17:52Z (GMT). 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dc.title.pt_BR.fl_str_mv Use of multiepitope polyproteins in serodiagnosis of active tuberculosis
dc.title.alternative.pt_BR.fl_str_mv Clinical and Diagnostic Laboratory Immunology
title Use of multiepitope polyproteins in serodiagnosis of active tuberculosis
spellingShingle Use of multiepitope polyproteins in serodiagnosis of active tuberculosis
Houghton, Raymond L.
title_short Use of multiepitope polyproteins in serodiagnosis of active tuberculosis
title_full Use of multiepitope polyproteins in serodiagnosis of active tuberculosis
title_fullStr Use of multiepitope polyproteins in serodiagnosis of active tuberculosis
title_full_unstemmed Use of multiepitope polyproteins in serodiagnosis of active tuberculosis
title_sort Use of multiepitope polyproteins in serodiagnosis of active tuberculosis
author Houghton, Raymond L.
author_facet Houghton, Raymond L.
Lodes, Michael J.
Dillon, Davin C.
Reynolds, Lisa D.
Day, Craig H.
Sampaio, Diana P.
Badaró, Roberto José da Silva
author_role author
author2 Lodes, Michael J.
Dillon, Davin C.
Reynolds, Lisa D.
Day, Craig H.
Sampaio, Diana P.
Badaró, Roberto José da Silva
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Houghton, Raymond L.
Lodes, Michael J.
Dillon, Davin C.
Reynolds, Lisa D.
Day, Craig H.
Sampaio, Diana P.
Badaró, Roberto José da Silva
Houghton, Raymond L.
Lodes, Michael J.
Dillon, Davin C.
Reynolds, Lisa D.
Day, Craig H.
Sampaio, Diana P.
Badaró, Roberto José da Silva
description Texto completo: acesso restrito. p. 883-891
publishDate 2002
dc.date.issued.fl_str_mv 2002-07
dc.date.accessioned.fl_str_mv 2012-12-12T17:17:52Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
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dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/7466
dc.identifier.issn.none.fl_str_mv 1071-412X
dc.identifier.number.pt_BR.fl_str_mv v. 9, n. 4
identifier_str_mv 1071-412X
v. 9, n. 4
url http://www.repositorio.ufba.br/ri/handle/ri/7466
dc.language.iso.fl_str_mv eng
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