Antifungal Potential of Terpenes from Spondias Purpurea L. Leaf Extract against Moniliophthora Perniciosa that causes Witches Broom Disease of Theobroma Cacao
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da UFBA |
Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/25932 |
Resumo: | The present in vitro study evaluates antifungal properties of Spondias purpurea plant extract against Moniliophthora perniciosa. Fractions of Ethanol Crude Extracts (CE) from S. purpurea were tested for their antifungal activity. CE inhibited mycelial growth of M. perniciosa by 60%at a dose of 10 mg/mL, while survival of broken hyphae of the fungus was inactivated by 90% at a dose of 8 mg/mL. Fractionation of CE yielded a terpene containing sub fraction (FA) with the highest fungicidal activity. FA acted as oxidant and its application induced oxidative stress in M. perniciosa that may be the responsible for the observed induced cell death. The chromatographic profile obtained with FA of S. purpurea revealed a variety of terpenes, which included Spathulenol (14.2%), Linolenic acid (8.4%), trans Caryophyllene (6.9%), and Alpha-muurolene (6.9%).This study describes for the first time effects of terpenes of S. purpurea related to induced oxidative stress in fungus. S. purpurea, therefore, may be a natural source for isolation of antifungal compounds against M. perniciosa. This characteristic may be useful in application as botanical fungicide in biological control. |
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Silva, Gabriele Marisco daSantos, Refineide XavierAguiar, Rosane MouraBrendel, MartinPungartinik, Cristina2018-05-04T14:28:47Z2018-05-04T14:28:47Z2018-05-04(2381-1803) International Journal of Complementary & Alternative Medicinehttp://repositorio.ufba.br/ri/handle/ri/259327The present in vitro study evaluates antifungal properties of Spondias purpurea plant extract against Moniliophthora perniciosa. Fractions of Ethanol Crude Extracts (CE) from S. purpurea were tested for their antifungal activity. CE inhibited mycelial growth of M. perniciosa by 60%at a dose of 10 mg/mL, while survival of broken hyphae of the fungus was inactivated by 90% at a dose of 8 mg/mL. Fractionation of CE yielded a terpene containing sub fraction (FA) with the highest fungicidal activity. FA acted as oxidant and its application induced oxidative stress in M. perniciosa that may be the responsible for the observed induced cell death. The chromatographic profile obtained with FA of S. purpurea revealed a variety of terpenes, which included Spathulenol (14.2%), Linolenic acid (8.4%), trans Caryophyllene (6.9%), and Alpha-muurolene (6.9%).This study describes for the first time effects of terpenes of S. purpurea related to induced oxidative stress in fungus. S. purpurea, therefore, may be a natural source for isolation of antifungal compounds against M. perniciosa. This characteristic may be useful in application as botanical fungicide in biological control.Submitted by Renorbio (renorbioba@ufba.br) on 2018-05-02T18:39:39Z No. of bitstreams: 1 ANTIFUNGAL POTENTIAL OF TERPENES FROM SPONDIAS PURPUREA L. 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LEAF EXTRACT AGAINST MONILIOPHTHORA PERNICIOSA THAT CAUSES WITCHES BROOM DISEASE OF THEOBROMA CACAO.pdf: 634671 bytes, checksum: 40c5b50b0071c7fd270b4bc3792ec57c (MD5)DOI: 10.15406/ijcam.2017.07.00215reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAMoniliophthora perniciosa;Spondias purpureaBiofungicideTerpenesNatural productsTheobroma cacaoAntifungal Potential of Terpenes from Spondias Purpurea L. Leaf Extract against Moniliophthora Perniciosa that causes Witches Broom Disease of Theobroma Cacaoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrasilinfo:eu-repo/semantics/openAccessengORIGINALANTIFUNGAL POTENTIAL OF TERPENES FROM SPONDIAS PURPUREA L. LEAF EXTRACT AGAINST MONILIOPHTHORA PERNICIOSA THAT CAUSES WITCHES BROOM DISEASE OF THEOBROMA CACAO.pdfANTIFUNGAL POTENTIAL OF TERPENES FROM SPONDIAS PURPUREA L. 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dc.title.pt_BR.fl_str_mv |
Antifungal Potential of Terpenes from Spondias Purpurea L. Leaf Extract against Moniliophthora Perniciosa that causes Witches Broom Disease of Theobroma Cacao |
title |
Antifungal Potential of Terpenes from Spondias Purpurea L. Leaf Extract against Moniliophthora Perniciosa that causes Witches Broom Disease of Theobroma Cacao |
spellingShingle |
Antifungal Potential of Terpenes from Spondias Purpurea L. Leaf Extract against Moniliophthora Perniciosa that causes Witches Broom Disease of Theobroma Cacao Silva, Gabriele Marisco da Moniliophthora perniciosa; Spondias purpurea Biofungicide Terpenes Natural products Theobroma cacao |
title_short |
Antifungal Potential of Terpenes from Spondias Purpurea L. Leaf Extract against Moniliophthora Perniciosa that causes Witches Broom Disease of Theobroma Cacao |
title_full |
Antifungal Potential of Terpenes from Spondias Purpurea L. Leaf Extract against Moniliophthora Perniciosa that causes Witches Broom Disease of Theobroma Cacao |
title_fullStr |
Antifungal Potential of Terpenes from Spondias Purpurea L. Leaf Extract against Moniliophthora Perniciosa that causes Witches Broom Disease of Theobroma Cacao |
title_full_unstemmed |
Antifungal Potential of Terpenes from Spondias Purpurea L. Leaf Extract against Moniliophthora Perniciosa that causes Witches Broom Disease of Theobroma Cacao |
title_sort |
Antifungal Potential of Terpenes from Spondias Purpurea L. Leaf Extract against Moniliophthora Perniciosa that causes Witches Broom Disease of Theobroma Cacao |
author |
Silva, Gabriele Marisco da |
author_facet |
Silva, Gabriele Marisco da Santos, Refineide Xavier Aguiar, Rosane Moura Brendel, Martin Pungartinik, Cristina |
author_role |
author |
author2 |
Santos, Refineide Xavier Aguiar, Rosane Moura Brendel, Martin Pungartinik, Cristina |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Silva, Gabriele Marisco da Santos, Refineide Xavier Aguiar, Rosane Moura Brendel, Martin Pungartinik, Cristina |
dc.subject.por.fl_str_mv |
Moniliophthora perniciosa; Spondias purpurea Biofungicide Terpenes Natural products Theobroma cacao |
topic |
Moniliophthora perniciosa; Spondias purpurea Biofungicide Terpenes Natural products Theobroma cacao |
description |
The present in vitro study evaluates antifungal properties of Spondias purpurea plant extract against Moniliophthora perniciosa. Fractions of Ethanol Crude Extracts (CE) from S. purpurea were tested for their antifungal activity. CE inhibited mycelial growth of M. perniciosa by 60%at a dose of 10 mg/mL, while survival of broken hyphae of the fungus was inactivated by 90% at a dose of 8 mg/mL. Fractionation of CE yielded a terpene containing sub fraction (FA) with the highest fungicidal activity. FA acted as oxidant and its application induced oxidative stress in M. perniciosa that may be the responsible for the observed induced cell death. The chromatographic profile obtained with FA of S. purpurea revealed a variety of terpenes, which included Spathulenol (14.2%), Linolenic acid (8.4%), trans Caryophyllene (6.9%), and Alpha-muurolene (6.9%).This study describes for the first time effects of terpenes of S. purpurea related to induced oxidative stress in fungus. S. purpurea, therefore, may be a natural source for isolation of antifungal compounds against M. perniciosa. This characteristic may be useful in application as botanical fungicide in biological control. |
publishDate |
2018 |
dc.date.accessioned.fl_str_mv |
2018-05-04T14:28:47Z |
dc.date.available.fl_str_mv |
2018-05-04T14:28:47Z |
dc.date.issued.fl_str_mv |
2018-05-04 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://repositorio.ufba.br/ri/handle/ri/25932 |
dc.identifier.issn.none.fl_str_mv |
(2381-1803) International Journal of Complementary & Alternative Medicine |
dc.identifier.number.pt_BR.fl_str_mv |
7 |
identifier_str_mv |
(2381-1803) International Journal of Complementary & Alternative Medicine 7 |
url |
http://repositorio.ufba.br/ri/handle/ri/25932 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.publisher.country.fl_str_mv |
Brasil |
dc.source.pt_BR.fl_str_mv |
DOI: 10.15406/ijcam.2017.07.00215 |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da UFBA instname:Universidade Federal da Bahia (UFBA) instacron:UFBA |
instname_str |
Universidade Federal da Bahia (UFBA) |
instacron_str |
UFBA |
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UFBA |
reponame_str |
Repositório Institucional da UFBA |
collection |
Repositório Institucional da UFBA |
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