Novel bisabolane derivative from “arnica-da-serra” (Vernonieae: Asteraceae) reduces pro-nociceptive cytokines levels in LPS-stimulated rat macrophages
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://repositorio.ufba.br/ri/handle/ri/13717 |
Resumo: | Texto completo. Acesso restrito. p. 993–998 |
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Pavarini, Daniel PetinattiNogueira, Elídia FernandesCallejon, Daniel RobertoSoares, Denis MeloSouza, Glória Emilia Petto deCunha, Fernando de QueirozLopes, João L. C.Lopes, Norberto PeporinePavarini, Daniel PetinattiNogueira, Elídia FernandesCallejon, Daniel RobertoSoares, Denis MeloSouza, Glória Emilia Petto deCunha, Fernando de QueirozLopes, João L. C.Lopes, Norberto Peporine2013-11-18T21:11:12Z20130378-8741http://repositorio.ufba.br/ri/handle/ri/13717v. 148, n. 3Texto completo. Acesso restrito. p. 993–998Ethnopharmacology relevance Hydro alcoholic leaves extracts (HALE) of Lychnophora ericoides Mart. (“false arnica” or “arnica-da-serra”) had been popularly used against pain and inflammatory process. Aim The present work aimed to look for possible active volatile compounds that could be found in HALE of Lychnophora ericoides among the non volatile anti-inflammatory and analgesic compounds previously reported. Methods Harvests were performed during the end of the wet summer season (April) when scented branches were instantly collected and frozen. HALE's were simulated at the lab by following the procedures lectured by the locals. Mass Spectrometry experiments suggested structural information when using both EI–MS and ESI–MS/MS. After isolation through classical thin layer chromatography (TLC) procedures, the NMR experiments and signals assignments were carried out. The effects on the cytokines or nitric oxide (NO) production were assessed at in vitro assays that had monitored the levels of these substances on the supernatant of LPS-stimulated macrophage primary cell culture. Results The major metabolite from HALE was isolated from the essential oil and the major compound had its molecular formulae established by Mass Spectrometry (High Resolution) and its structure by NMR. Literature-based investigation enables us to define the structure of the new metabolite as 6-methyl-2-(4-methylcyclohex-4-enyl-2-acetyloxy) hept-5-en-2-ol and its name as orto-acetoxy-bisabolol. In vitro assay of interleukins release inhibition was carried out using rat peritoneal macrophages cultures. IL-1β and TNF-α levels were significantly reduced when cells were previously treated with low doses of orto-acetoxy-bisabolol, but neither IL-6 nor NO levels have their levels reduced. Results suggest that ethnical knowledge of anti-inflammatory and analgesic effects of the “arnica-da-serra” HALE may be associated to the orto-acetoxy-bisabolol ability on synthesis inhibition of the key inflammatory/hypernociceptive mediators. Conclusions Phytochemical investigation of the volatile active compounds in Lychnophora ericoides HALE allows us to isolate a new bisabolane derivative (orto-acetoxy-bisabolol) and to infer that this compound inhibits the synthesis of TNF-α and IL-1β, two important inflammatory mediators in the hypernociception. Our present data, in addition to literature's data, furnish scientific support to folk's use of Lychnophora ericoides as an endemic wound healer.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-10-15T19:41:24Z No. of bitstreams: 1 7777777777.pdf: 477284 bytes, checksum: 40c77d9a7dda891cb47f950b94ae6685 (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2013-11-18T21:11:12Z (GMT) No. of bitstreams: 1 7777777777.pdf: 477284 bytes, checksum: 40c77d9a7dda891cb47f950b94ae6685 (MD5)Made available in DSpace on 2013-11-18T21:11:12Z (GMT). No. of bitstreams: 1 7777777777.pdf: 477284 bytes, checksum: 40c77d9a7dda891cb47f950b94ae6685 (MD5) Previous issue date: 2013SalvadorJournal of Ethnopharmacologyhttp://www.sciencedirect.com/science/article/pii/S0378874113003383reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAArnica-da-serraAnti-inflammatoryOrto-acetoxy-bisabololAsteraceaeInterleukinesNitric oxideNovel bisabolane derivative from “arnica-da-serra” (Vernonieae: Asteraceae) reduces pro-nociceptive cytokines levels in LPS-stimulated rat macrophagesJournal of Ethnopharmacologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengLICENSElicense.txtlicense.txttext/plain1365https://repositorio.ufba.br/bitstream/ri/13717/2/license.txt5371a150bdc863f78dcf39281543bd86MD52ORIGINAL7777777777.pdf7777777777.pdfapplication/pdf477284https://repositorio.ufba.br/bitstream/ri/13717/1/7777777777.pdf40c77d9a7dda891cb47f950b94ae6685MD51TEXT7777777777.pdf.txt7777777777.pdf.txtExtracted texttext/plain33764https://repositorio.ufba.br/bitstream/ri/13717/3/7777777777.pdf.txt38e9baaf18081061ebaf4a4f4179d0b9MD53ri/137172022-07-05 14:03:24.05oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:24Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Novel bisabolane derivative from “arnica-da-serra” (Vernonieae: Asteraceae) reduces pro-nociceptive cytokines levels in LPS-stimulated rat macrophages |
| dc.title.alternative.pt_BR.fl_str_mv |
Journal of Ethnopharmacology |
| title |
Novel bisabolane derivative from “arnica-da-serra” (Vernonieae: Asteraceae) reduces pro-nociceptive cytokines levels in LPS-stimulated rat macrophages |
| spellingShingle |
Novel bisabolane derivative from “arnica-da-serra” (Vernonieae: Asteraceae) reduces pro-nociceptive cytokines levels in LPS-stimulated rat macrophages Pavarini, Daniel Petinatti Arnica-da-serra Anti-inflammatory Orto-acetoxy-bisabolol Asteraceae Interleukines Nitric oxide |
| title_short |
Novel bisabolane derivative from “arnica-da-serra” (Vernonieae: Asteraceae) reduces pro-nociceptive cytokines levels in LPS-stimulated rat macrophages |
| title_full |
Novel bisabolane derivative from “arnica-da-serra” (Vernonieae: Asteraceae) reduces pro-nociceptive cytokines levels in LPS-stimulated rat macrophages |
| title_fullStr |
Novel bisabolane derivative from “arnica-da-serra” (Vernonieae: Asteraceae) reduces pro-nociceptive cytokines levels in LPS-stimulated rat macrophages |
| title_full_unstemmed |
Novel bisabolane derivative from “arnica-da-serra” (Vernonieae: Asteraceae) reduces pro-nociceptive cytokines levels in LPS-stimulated rat macrophages |
| title_sort |
Novel bisabolane derivative from “arnica-da-serra” (Vernonieae: Asteraceae) reduces pro-nociceptive cytokines levels in LPS-stimulated rat macrophages |
| author |
Pavarini, Daniel Petinatti |
| author_facet |
Pavarini, Daniel Petinatti Nogueira, Elídia Fernandes Callejon, Daniel Roberto Soares, Denis Melo Souza, Glória Emilia Petto de Cunha, Fernando de Queiroz Lopes, João L. C. Lopes, Norberto Peporine |
| author_role |
author |
| author2 |
Nogueira, Elídia Fernandes Callejon, Daniel Roberto Soares, Denis Melo Souza, Glória Emilia Petto de Cunha, Fernando de Queiroz Lopes, João L. C. Lopes, Norberto Peporine |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Pavarini, Daniel Petinatti Nogueira, Elídia Fernandes Callejon, Daniel Roberto Soares, Denis Melo Souza, Glória Emilia Petto de Cunha, Fernando de Queiroz Lopes, João L. C. Lopes, Norberto Peporine Pavarini, Daniel Petinatti Nogueira, Elídia Fernandes Callejon, Daniel Roberto Soares, Denis Melo Souza, Glória Emilia Petto de Cunha, Fernando de Queiroz Lopes, João L. C. Lopes, Norberto Peporine |
| dc.subject.por.fl_str_mv |
Arnica-da-serra Anti-inflammatory Orto-acetoxy-bisabolol Asteraceae Interleukines Nitric oxide |
| topic |
Arnica-da-serra Anti-inflammatory Orto-acetoxy-bisabolol Asteraceae Interleukines Nitric oxide |
| description |
Texto completo. Acesso restrito. p. 993–998 |
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2013 |
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2013-11-18T21:11:12Z |
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2013 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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0378-8741 |
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v. 148, n. 3 |
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0378-8741 v. 148, n. 3 |
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Journal of Ethnopharmacology |
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Journal of Ethnopharmacology |
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