Identification of seroreactive proteins of Leptospira interrogans Serovar Copenhageni using a High-Density protein Microarray approach

Detalhes bibliográficos
Autor(a) principal: Aquino, Carolina Lessa
Data de Publicação: 2013
Outros Autores: Rodrigues, Camila Borges, Pablo, Jozelyn, Sasaki, Rie, Jasinskas, Algis, Ribeiro, Guilherme S., Vigil, Adam
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/14881
Resumo: p. 1-13
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spelling Aquino, Carolina LessaRodrigues, Camila BorgesPablo, JozelynSasaki, RieJasinskas, AlgisRibeiro, Guilherme S.Vigil, AdamAquino, Carolina LessaRodrigues, Camila BorgesPablo, JozelynSasaki, RieJasinskas, AlgisRibeiro, Guilherme S.Vigil, Adam2014-04-29T14:48:23Z2014-04-29T14:48:23Z20131935-2727http://repositorio.ufba.br/ri/handle/ri/14881v. 7, n. 10p. 1-13Background: Leptospirosis is a widespread zoonotic disease worldwide. The lack of an adequate laboratory test is a major barrier for diagnosis, especially during the early stages of illness, when antibiotic therapy is most effective. Therefore, there is a critical need for an efficient diagnostic test for this life threatening disease. Methodology: In order to identify new targets that could be used as diagnostic makers for leptopirosis, we constructed a protein microarray chip comprising 61% of Leptospira interrogans proteome and investigated the IgG response from 274 individuals, including 80 acute-phase, 80 convalescent-phase patients and 114 healthy control subjects from regions with endemic, high endemic, and no endemic transmission of leptospirosis. A nitrocellulose line blot assay was performed to validate the accuracy of the protein microarray results. Principal findings: We found 16 antigens that can discriminate between acute cases and healthy individuals from a region with high endemic transmission of leptospirosis, and 18 antigens that distinguish convalescent cases. Some of the antigens identified in this study, such as LipL32, the non-identical domains of the Lig proteins, GroEL, and Loa22 are already known to be recognized by sera from human patients, thus serving as proof-of-concept for the serodiagnostic antigen discovery approach. Several novel antigens were identified, including the hypothetical protein LIC10215 which showed good sensitivity and specificity rates for both acute- and convalescent-phase patients. Conclusions: Our study is the first large-scale evaluation of immunodominant antigens associated with naturally acquired leptospiral infection, and novel as well as known serodiagnostic leptospiral antigens that are recognized by antibodies in the sera of leptospirosis cases were identified. The novel antigens identified here may have potential use in both the development of new tests and the improvement of currently available assays for diagnosing this neglected tropical disease. Further research is needed to assess the utility of these antigens in more deployable diagnostic platforms.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2014-04-29T14:48:23Z No. of bitstreams: 1 10.1371journal.pntd.0002499.pdf: 3098624 bytes, checksum: 826a0e01117159a2da74e3d62b123c25 (MD5)Made available in DSpace on 2014-04-29T14:48:23Z (GMT). No. of bitstreams: 1 10.1371journal.pntd.0002499.pdf: 3098624 bytes, checksum: 826a0e01117159a2da74e3d62b123c25 (MD5) Previous issue date: 2013http://dx.doi.org/10.1371/journal.pntd.0002499reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAIdentification of seroreactive proteins of Leptospira interrogans Serovar Copenhageni using a High-Density protein Microarray approachPLoS Neglected Tropical Diseasesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleBrasilinfo:eu-repo/semantics/openAccessengORIGINAL10.1371journal.pntd.0002499.pdf10.1371journal.pntd.0002499.pdfapplication/pdf3098624https://repositorio.ufba.br/bitstream/ri/14881/1/10.1371journal.pntd.0002499.pdf826a0e01117159a2da74e3d62b123c25MD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/14881/2/license.txt0d4b811ef71182510d2015daa7c8a900MD52TEXT10.1371journal.pntd.0002499.pdf.txt10.1371journal.pntd.0002499.pdf.txtExtracted texttext/plain63937https://repositorio.ufba.br/bitstream/ri/14881/3/10.1371journal.pntd.0002499.pdf.txt6eca95fff083e95fa0f2b3f205e028cbMD53ri/148812022-08-08 13:17:30.793oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:17:30Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Identification of seroreactive proteins of Leptospira interrogans Serovar Copenhageni using a High-Density protein Microarray approach
dc.title.alternative.pt_BR.fl_str_mv PLoS Neglected Tropical Diseases
title Identification of seroreactive proteins of Leptospira interrogans Serovar Copenhageni using a High-Density protein Microarray approach
spellingShingle Identification of seroreactive proteins of Leptospira interrogans Serovar Copenhageni using a High-Density protein Microarray approach
Aquino, Carolina Lessa
title_short Identification of seroreactive proteins of Leptospira interrogans Serovar Copenhageni using a High-Density protein Microarray approach
title_full Identification of seroreactive proteins of Leptospira interrogans Serovar Copenhageni using a High-Density protein Microarray approach
title_fullStr Identification of seroreactive proteins of Leptospira interrogans Serovar Copenhageni using a High-Density protein Microarray approach
title_full_unstemmed Identification of seroreactive proteins of Leptospira interrogans Serovar Copenhageni using a High-Density protein Microarray approach
title_sort Identification of seroreactive proteins of Leptospira interrogans Serovar Copenhageni using a High-Density protein Microarray approach
author Aquino, Carolina Lessa
author_facet Aquino, Carolina Lessa
Rodrigues, Camila Borges
Pablo, Jozelyn
Sasaki, Rie
Jasinskas, Algis
Ribeiro, Guilherme S.
Vigil, Adam
author_role author
author2 Rodrigues, Camila Borges
Pablo, Jozelyn
Sasaki, Rie
Jasinskas, Algis
Ribeiro, Guilherme S.
Vigil, Adam
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Aquino, Carolina Lessa
Rodrigues, Camila Borges
Pablo, Jozelyn
Sasaki, Rie
Jasinskas, Algis
Ribeiro, Guilherme S.
Vigil, Adam
Aquino, Carolina Lessa
Rodrigues, Camila Borges
Pablo, Jozelyn
Sasaki, Rie
Jasinskas, Algis
Ribeiro, Guilherme S.
Vigil, Adam
description p. 1-13
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2014-04-29T14:48:23Z
dc.date.available.fl_str_mv 2014-04-29T14:48:23Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/14881
dc.identifier.issn.none.fl_str_mv 1935-2727
dc.identifier.number.pt_BR.fl_str_mv v. 7, n. 10
identifier_str_mv 1935-2727
v. 7, n. 10
url http://repositorio.ufba.br/ri/handle/ri/14881
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.country.fl_str_mv Brasil
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1371/journal.pntd.0002499
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