Sub-clinical infection as an effective protocol for obtaining anti-Leishmania chagasi amastigote antibodies of different animal species

Detalhes bibliográficos
Autor(a) principal: Fróes, Adriana M.
Data de Publicação: 2004
Outros Autores: Santos, Cláudia V. D. dos, Penha-Filho, Manoel L., Teixeira, Márcia Cristina Aquino, Silva, Tânia Maria Correia, Oliveira, Geraldo G.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/7475
Resumo: p. 135-141
id UFBA-2_9e4f7971e5eb65d33c89ecb2a804592c
oai_identifier_str oai:repositorio.ufba.br:ri/7475
network_acronym_str UFBA-2
network_name_str Repositório Institucional da UFBA
repository_id_str 1932
spelling Fróes, Adriana M.Santos, Cláudia V. D. dosPenha-Filho, Manoel L.Teixeira, Márcia Cristina AquinoSilva, Tânia Maria CorreiaOliveira, Geraldo G.Fróes, Adriana M.Santos, Cláudia V. D. dosPenha-Filho, Manoel L.Teixeira, Márcia Cristina AquinoSilva, Tânia Maria CorreiaOliveira, Geraldo G.2012-12-12T19:44:40Z20040165-2427http://www.repositorio.ufba.br/ri/handle/ri/7475v. 99, n. 3-4p. 135-141This work aims at identifying an effective protocol to raise anti-Leishmania chagasi amastigote antibodies in different animal species. Protocols of immunization by subcutaneous injections of L. chagasi promastigote and amastigote lysates or by either intravenous or subcutaneous inoculation of live metacyclic promastigotes were assessed in mice, rabbits, and dogs. The immunization with live promastigotes produced a strong humoral immune response against L. chagasi amastigotes in all three animal species. The sera from animals immunized with the promastigote lysate did not react with amastigotes and, conversely, the sera from mice immunized with the amastigote lysate did not react with promastigotes. Taken all data together, the immunization through infection with metacyclic promastigotes was considered the most satisfactory way to immunize animals for obtaining anti-amastigote and anti-promastigote antibodies, since it did not only allowed the obtention of antibody against the two forms of the parasite, but it is also cheap, less laborious than carrying out the purification of amastigotes from infected tissues and avoid the use of a large number of hamsters for obtention the amastigotes, necessary to produce the immunogenic lysates. Furthermore, this immunization protocol was comparable to the amastigote lysate immunization protocol for the obtaining of mouse monoclonal antibodies (mAbs).Submitted by JURANDI DE SOUZA SILVA (jssufba@hotmail.com) on 2012-12-12T19:44:40Z No. of bitstreams: 1 1-s2.0-S0165242704000303-main.pdf: 196838 bytes, checksum: 2469cce22e5a85cc72f5fe49ac74c1fc (MD5)Made available in DSpace on 2012-12-12T19:44:40Z (GMT). No. of bitstreams: 1 1-s2.0-S0165242704000303-main.pdf: 196838 bytes, checksum: 2469cce22e5a85cc72f5fe49ac74c1fc (MD5) Previous issue date: 2004http://dx.doi.org/10.1016/j.vetimm.2004.01.013reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAImmune responseLeishmania chagasi-amastigotesAntibodies-monoclonalImmunohistologySub-clinical infection as an effective protocol for obtaining anti-Leishmania chagasi amastigote antibodies of different animal speciesVeterinary Immunology and Immunopathologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01enginfo:eu-repo/semantics/openAccessORIGINAL1-s2.0-S0165242704000303-main.pdf1-s2.0-S0165242704000303-main.pdfapplication/pdf196838https://repositorio.ufba.br/bitstream/ri/7475/1/1-s2.0-S0165242704000303-main.pdf2469cce22e5a85cc72f5fe49ac74c1fcMD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/7475/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT1-s2.0-S0165242704000303-main.pdf.txt1-s2.0-S0165242704000303-main.pdf.txtExtracted texttext/plain25609https://repositorio.ufba.br/bitstream/ri/7475/3/1-s2.0-S0165242704000303-main.pdf.txt911e234a961b6abdd108391d038b7620MD53ri/74752022-07-05 14:03:43.287oai:repositorio.ufba.br:ri/7475VGVybW8gZGUgTGljZW7vv71hLCBu77+9byBleGNsdXNpdm8sIHBhcmEgbyBkZXDvv71zaXRvIG5vIHJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVRkJBCgogICAgUGVsbyBwcm9jZXNzbyBkZSBzdWJtaXNz77+9byBkZSBkb2N1bWVudG9zLCBvIGF1dG9yIG91IHNldQpyZXByZXNlbnRhbnRlIGxlZ2FsLCBhbyBhY2VpdGFyIGVzc2UgdGVybW8gZGUgbGljZW7vv71hLCBjb25jZWRlIGFvClJlcG9zaXTvv71yaW8gSW5zdGl0dWNpb25hbCBkYSBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkYSBCYWhpYSBvIGRpcmVpdG8KZGUgbWFudGVyIHVtYSBj77+9cGlhIGVtIHNldSByZXBvc2l077+9cmlvIGNvbSBhIGZpbmFsaWRhZGUsIHByaW1laXJhLCAKZGUgcHJlc2VydmHvv73vv71vLiBFc3NlcyB0ZXJtb3MsIG7vv71vIGV4Y2x1c2l2b3MsIG1hbnTvv71tIG9zIGRpcmVpdG9zIGRlIAphdXRvci9jb3B5cmlnaHQsIG1hcyBlbnRlbmRlIG8gZG9jdW1lbnRvIGNvbW8gcGFydGUgZG8gYWNlcnZvIGludGVsZWN0dWFsIGRlc3NhIFVuaXZlcnNpZGFkZS4gCgogICAgUGFyYSBvcyBkb2N1bWVudG9zIHB1YmxpY2Fkb3MgY29tIHJlcGFzc2UgZGUgZGlyZWl0b3MgZGUgZGlzdHJpYnVp77+977+9bywgZXNzZSB0ZXJtbyBkZSBsaWNlbu+/vWEgZW50ZW5kZSBxdWU6IAoKICAgIE1hbnRlbmRvIG9zICBkaXJlaXRvcyBhdXRvcmFpcywgcmVwYXNzYWRvcyBhIHRlcmNlaXJvcywgZW0gY2FzbyAKZGUgcHVibGljYe+/ve+/vWVzLCBvIHJlcG9zaXTvv71yaW8gcG9kZSByZXN0cmluZ2lyIG8gYWNlc3NvIGFvIHRleHRvIAppbnRlZ3JhbCwgbWFzIGxpYmVyYSBhcyBpbmZvcm1h77+977+9ZXMgc29icmUgbyBkb2N1bWVudG8gKE1ldGFkYWRvcyBkZXNjcml0aXZvcykuCgogRGVzdGEgZm9ybWEsIGF0ZW5kZW5kbyBhb3MgYW5zZWlvcyBkZXNzYSB1bml2ZXJzaWRhZGUgCmVtIG1hbnRlciBzdWEgcHJvZHXvv73vv71vIGNpZW5077+9ZmljYSBjb20gYXMgcmVzdHJp77+977+9ZXMgaW1wb3N0YXMgcGVsb3MgCmVkaXRvcmVzIGRlIHBlcmnvv71kaWNvcy4gCgogICAgUGFyYSBhcyBwdWJsaWNh77+977+9ZXMgZW0gaW5pY2lhdGl2YXMgcXVlIHNlZ3VlbSBhIHBvbO+/vXRpY2EgZGUgCkFjZXNzbyBBYmVydG8sIG9zIGRlcO+/vXNpdG9zIGNvbXB1bHPvv71yaW9zIG5lc3NlIHJlcG9zaXTvv71yaW8gbWFudO+/vW0gCm9zIGRpcmVpdG9zIGF1dG9yYWlzLCBtYXMgbWFudO+/vW0gbyBhY2Vzc28gaXJyZXN0cml0byBhbyBtZXRhZGFkb3MgCmUgdGV4dG8gY29tcGxldG8uIEFzc2ltLCBhIGFjZWl0Ye+/ve+/vW8gZGVzc2UgdGVybW8gbu+/vW8gbmVjZXNzaXRhIGRlIApjb25zZW50aW1lbnRvIHBvciBwYXJ0ZSBkZSBhdXRvcmVzL2RldGVudG9yZXMgZG9zIGRpcmVpdG9zLCBwb3IgCmVzdGFyZW0gZW0gaW5pY2lhdGl2YXMgZGUgYWNlc3NvIGFiZXJ0by4KCiAgICBFbSBhbWJvcyBvIGNhc28sIGVzc2UgdGVybW8gZGUgbGljZW7vv71hLCBwb2RlIHNlciBhY2VpdG8gcGVsbyAKYXV0b3IsIGRldGVudG9yZXMgZGUgZGlyZWl0b3MgZS9vdSB0ZXJjZWlyb3MgYW1wYXJhZG9zIHBlbGEgCnVuaXZlcnNpZGFkZS4gRGV2aWRvIGFvcyBkaWZlcmVudGVzIHByb2Nlc3NvcyBwZWxvIHF1YWwgYSBzdWJtaXNz77+9byAKcG9kZSBvY29ycmVyLCBvIHJlcG9zaXTvv71yaW8gcGVybWl0ZSBhIGFjZWl0Ye+/ve+/vW8gZGEgbGljZW7vv71hIHBvciAKdGVyY2Vpcm9zLCBzb21lbnRlIG5vcyBjYXNvcyBkZSBkb2N1bWVudG9zIHByb2R1emlkb3MgcG9yIGludGVncmFudGVzIApkYSBVRkJBIGUgc3VibWV0aWRvcyBwb3IgcGVzc29hcyBhbXBhcmFkYXMgcG9yIGVzdGEgaW5zdGl0dWnvv73vv71vLgo=Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:43Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Sub-clinical infection as an effective protocol for obtaining anti-Leishmania chagasi amastigote antibodies of different animal species
dc.title.alternative.pt_BR.fl_str_mv Veterinary Immunology and Immunopathology
title Sub-clinical infection as an effective protocol for obtaining anti-Leishmania chagasi amastigote antibodies of different animal species
spellingShingle Sub-clinical infection as an effective protocol for obtaining anti-Leishmania chagasi amastigote antibodies of different animal species
Fróes, Adriana M.
Immune response
Leishmania chagasi-amastigotes
Antibodies-monoclonal
Immunohistology
title_short Sub-clinical infection as an effective protocol for obtaining anti-Leishmania chagasi amastigote antibodies of different animal species
title_full Sub-clinical infection as an effective protocol for obtaining anti-Leishmania chagasi amastigote antibodies of different animal species
title_fullStr Sub-clinical infection as an effective protocol for obtaining anti-Leishmania chagasi amastigote antibodies of different animal species
title_full_unstemmed Sub-clinical infection as an effective protocol for obtaining anti-Leishmania chagasi amastigote antibodies of different animal species
title_sort Sub-clinical infection as an effective protocol for obtaining anti-Leishmania chagasi amastigote antibodies of different animal species
author Fróes, Adriana M.
author_facet Fróes, Adriana M.
Santos, Cláudia V. D. dos
Penha-Filho, Manoel L.
Teixeira, Márcia Cristina Aquino
Silva, Tânia Maria Correia
Oliveira, Geraldo G.
author_role author
author2 Santos, Cláudia V. D. dos
Penha-Filho, Manoel L.
Teixeira, Márcia Cristina Aquino
Silva, Tânia Maria Correia
Oliveira, Geraldo G.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Fróes, Adriana M.
Santos, Cláudia V. D. dos
Penha-Filho, Manoel L.
Teixeira, Márcia Cristina Aquino
Silva, Tânia Maria Correia
Oliveira, Geraldo G.
Fróes, Adriana M.
Santos, Cláudia V. D. dos
Penha-Filho, Manoel L.
Teixeira, Márcia Cristina Aquino
Silva, Tânia Maria Correia
Oliveira, Geraldo G.
dc.subject.por.fl_str_mv Immune response
Leishmania chagasi-amastigotes
Antibodies-monoclonal
Immunohistology
topic Immune response
Leishmania chagasi-amastigotes
Antibodies-monoclonal
Immunohistology
description p. 135-141
publishDate 2004
dc.date.issued.fl_str_mv 2004
dc.date.accessioned.fl_str_mv 2012-12-12T19:44:40Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/7475
dc.identifier.issn.none.fl_str_mv 0165-2427
dc.identifier.number.pt_BR.fl_str_mv v. 99, n. 3-4
identifier_str_mv 0165-2427
v. 99, n. 3-4
url http://www.repositorio.ufba.br/ri/handle/ri/7475
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.vetimm.2004.01.013
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
bitstream.url.fl_str_mv https://repositorio.ufba.br/bitstream/ri/7475/1/1-s2.0-S0165242704000303-main.pdf
https://repositorio.ufba.br/bitstream/ri/7475/2/license.txt
https://repositorio.ufba.br/bitstream/ri/7475/3/1-s2.0-S0165242704000303-main.pdf.txt
bitstream.checksum.fl_str_mv 2469cce22e5a85cc72f5fe49ac74c1fc
1b89a9a0548218172d7c829f87a0eab9
911e234a961b6abdd108391d038b7620
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)
repository.mail.fl_str_mv
_version_ 1808459412919025664

Registros relacionados