Use of RT–PCR on oral fluid samples to assist the identification of measles cases during an outbreak

Detalhes bibliográficos
Autor(a) principal: Oliveira, S. A.
Data de Publicação: 2003
Outros Autores: Siqueira, M. M., Camacho, Luiz Antonio Bastos, Silva, R. Castro, Bruno, B. F., Cohen, B. J.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/7323
Resumo: p. 101-106
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spelling Oliveira, S. A.Siqueira, M. M.Camacho, Luiz Antonio BastosSilva, R. CastroBruno, B. F.Cohen, B. J.Oliveira, S. A.Siqueira, M. M.Camacho, Luiz Antonio BastosSilva, R. CastroBruno, B. F.Cohen, B. J.2012-12-07T15:07:15Z2012-12-07T15:07:15Z20030950-2688http://www.repositorio.ufba.br/ri/handle/ri/7323v. 130, n. 1p. 101-106This study investigated the occurrence of mild modified measles cases during an outbreak in Niterói, RJ, Brazil by using RT–PCR on oral fluid samples. From August to December 1997 a total of 76 patients with rash were seen at the study sites. Confirmed diagnosis by serology was achieved in 47 cases: measles (39·5%), rubella (13·2%), HHV-6 (3·9%), human parvovirus B19 (3·9%), dengue fever (3%). For 19 of the 29 patients without a conclusive diagnosis paired serum and saliva samples were available for further tests. In four of them, measles virus RNA was detected by RT–PCR in saliva samples in the absence of specific IgM in serum samples. Vaccination histories obtained from three of the RT–PCR positive cases showed that individuals previously immunized can still be infected and contribute to the circulation of measles virus. This study demonstrated the usefulness of RT–PCR on non-invasive clinical samples for the investigation of measles cases.Submitted by Reis Suelen (suelen_suzane@hotmail.com) on 2012-12-07T15:07:15Z No. of bitstreams: 1 download.pdf: 548163 bytes, checksum: 6b416645b7fe1bb9270fc1b0413edd93 (MD5)Made available in DSpace on 2012-12-07T15:07:15Z (GMT). No. of bitstreams: 1 download.pdf: 548163 bytes, checksum: 6b416645b7fe1bb9270fc1b0413edd93 (MD5) Previous issue date: 2003http://dx.doi.org/10.1017/S0950268802007963reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAUse of RT–PCR on oral fluid samples to assist the identification of measles cases during an outbreakEpidemiology and Infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessORIGINALdownload.pdfdownload.pdfapplication/pdf548163https://repositorio.ufba.br/bitstream/ri/7323/1/download.pdf6b416645b7fe1bb9270fc1b0413edd93MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/7323/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXTdownload.pdf.txtdownload.pdf.txtExtracted texttext/plain26779https://repositorio.ufba.br/bitstream/ri/7323/3/download.pdf.txtb74dea43d90ec082aa29a1c3125804f3MD53ri/73232022-07-05 14:03:23.155oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:23Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Use of RT–PCR on oral fluid samples to assist the identification of measles cases during an outbreak
dc.title.alternative.pt_BR.fl_str_mv Epidemiology and Infection
title Use of RT–PCR on oral fluid samples to assist the identification of measles cases during an outbreak
spellingShingle Use of RT–PCR on oral fluid samples to assist the identification of measles cases during an outbreak
Oliveira, S. A.
title_short Use of RT–PCR on oral fluid samples to assist the identification of measles cases during an outbreak
title_full Use of RT–PCR on oral fluid samples to assist the identification of measles cases during an outbreak
title_fullStr Use of RT–PCR on oral fluid samples to assist the identification of measles cases during an outbreak
title_full_unstemmed Use of RT–PCR on oral fluid samples to assist the identification of measles cases during an outbreak
title_sort Use of RT–PCR on oral fluid samples to assist the identification of measles cases during an outbreak
author Oliveira, S. A.
author_facet Oliveira, S. A.
Siqueira, M. M.
Camacho, Luiz Antonio Bastos
Silva, R. Castro
Bruno, B. F.
Cohen, B. J.
author_role author
author2 Siqueira, M. M.
Camacho, Luiz Antonio Bastos
Silva, R. Castro
Bruno, B. F.
Cohen, B. J.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Oliveira, S. A.
Siqueira, M. M.
Camacho, Luiz Antonio Bastos
Silva, R. Castro
Bruno, B. F.
Cohen, B. J.
Oliveira, S. A.
Siqueira, M. M.
Camacho, Luiz Antonio Bastos
Silva, R. Castro
Bruno, B. F.
Cohen, B. J.
description p. 101-106
publishDate 2003
dc.date.issued.fl_str_mv 2003
dc.date.accessioned.fl_str_mv 2012-12-07T15:07:15Z
dc.date.available.fl_str_mv 2012-12-07T15:07:15Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/7323
dc.identifier.issn.none.fl_str_mv 0950-2688
dc.identifier.number.pt_BR.fl_str_mv v. 130, n. 1
identifier_str_mv 0950-2688
v. 130, n. 1
url http://www.repositorio.ufba.br/ri/handle/ri/7323
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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