Alkaloids from Prosopis juliflora leaves induce glial activation, cytotoxicity and stimulate NO production
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2007 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/5245 |
Resumo: | p.601–614 |
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Silva, Andre Mario Mendes daSilva, Ana RitaPinheiro, Alexandre MoraesFreitas, Sandra Regina Villas Bôas deSilva, Victor Diogenes Amaral daSouza, Cleide dos SantosHughes, Juliana BentesEl-Bachá, Ramon dos SantosCosta, Maria de Fátima DiasVelozo, Eudes da SilvaTardy, Marcienne BlochCosta, Silvia LimaSilva, Andre Mario Mendes daSilva, Ana RitaPinheiro, Alexandre MoraesFreitas, Sandra Regina Villas Bôas deSilva, Victor Diogenes Amaral daSouza, Cleide dos SantosHughes, Juliana BentesEl-Bachá, Ramon dos SantosCosta, Maria de Fátima DiasVelozo, Eudes da SilvaTardy, Marcienne BlochCosta, Silvia Lima2012-01-25T11:59:51Z2012-01-25T11:59:51Z20070041-0101http://www.repositorio.ufba.br/ri/handle/ri/5245v. 49.p.601–614Prosopis juliflora is used for feeding cattle and humans. Intoxication with the plant has been reported, and is characterized by neuromuscular alterations and gliosis. Total alkaloidal extract (TAE) was obtained using acid/basicmodified extraction and was fractionated. TAE and seven alkaloidal fractions, at concentrations ranging 0.03–30 mg/ml, were tested for 24 h on astrocyte primary cultures derived from the cortex of newborn Wistar rats. The MTT test and the measure of LDH activity on the culture medium, revealed that TAE and fractions F29/30, F31/33, F32 and F34/35 were cytotoxic to astrocytes. The EC50 values for the most toxic compounds, TAE, F31/33 and F32 were 2.87 2.82 and 3.01 mg/ml, respectively. Morphological changes and glial cells activation were investigated through Rosenfeld’s staining, by immunocytochemistry for the protein OX-42, specific of activated microglia, by immunocytochemistry and western immunoblot for GFAP, the marker of reactive and mature astrocytes, and by the production of nitric oxide (NO). We observed that astrocytes exposed to 3 mg/ml TAE, F29/30 or F31/33 developed compact cell body with many processes overexpressing GFAP. Treatment with 30 mg/ml TAE and fractions, induced cytotoxicity characterized by a strong cell body contraction, very thin and long processes and condensed chromatin. We also observed that when compared with the control (71.34%), the proportion of OX-42 positive cells was increased in cultures treated with 30 mg/ml TAE or F29/30, F31/33, F32 and F34/35, with values raging from 7.27% to 28.74%. Moreover, incubation with 3 mg/ml F32, 30 mg/ml TAE, F29/30, F31/33 or F34/35 induced accumulation of nitrite in culture medium indicating induction of NO production. Taken together these results show that TAE and fractionated alkaloids from P. juliflora act directly on glial cells, inducing activation and/or cytotoxicity, stimulating NO production, and may have an impact on neuronal damages observed on intoxicated animals.Submitted by Ana Valéria de Jesus Moura (anavaleria_131@hotmail.com) on 2012-01-25T11:59:51Z No. of bitstreams: 1 S0041010106002935-main.pdf: 3107705 bytes, checksum: d8e8f9aca8faff983ff4a82e6bea66ce (MD5)Made available in DSpace on 2012-01-25T11:59:51Z (GMT). No. of bitstreams: 1 S0041010106002935-main.pdf: 3107705 bytes, checksum: d8e8f9aca8faff983ff4a82e6bea66ce (MD5) Previous issue date: 2007Prosopis julifloraAlkaloidsAstrocytesMicrogliaGFAPNitric oxideAlkaloids from Prosopis juliflora leaves induce glial activation, cytotoxicity and stimulate NO productionToxiconinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleengreponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAinfo:eu-repo/semantics/openAccessORIGINALS0041010106002935-main.pdfS0041010106002935-main.pdfapplication/pdf3107705https://repositorio.ufba.br/bitstream/ri/5245/1/S0041010106002935-main.pdfd8e8f9aca8faff983ff4a82e6bea66ceMD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/5245/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXTS0041010106002935-main.pdf.txtS0041010106002935-main.pdf.txtExtracted texttext/plain48700https://repositorio.ufba.br/bitstream/ri/5245/3/S0041010106002935-main.pdf.txt7d94e30bede1bef9856b5392110c5730MD53ri/52452022-07-05 14:03:16.166oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:03:16Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Alkaloids from Prosopis juliflora leaves induce glial activation, cytotoxicity and stimulate NO production |
| dc.title.alternative.pt_BR.fl_str_mv |
Toxicon |
| title |
Alkaloids from Prosopis juliflora leaves induce glial activation, cytotoxicity and stimulate NO production |
| spellingShingle |
Alkaloids from Prosopis juliflora leaves induce glial activation, cytotoxicity and stimulate NO production Silva, Andre Mario Mendes da Prosopis juliflora Alkaloids Astrocytes Microglia GFAP Nitric oxide |
| title_short |
Alkaloids from Prosopis juliflora leaves induce glial activation, cytotoxicity and stimulate NO production |
| title_full |
Alkaloids from Prosopis juliflora leaves induce glial activation, cytotoxicity and stimulate NO production |
| title_fullStr |
Alkaloids from Prosopis juliflora leaves induce glial activation, cytotoxicity and stimulate NO production |
| title_full_unstemmed |
Alkaloids from Prosopis juliflora leaves induce glial activation, cytotoxicity and stimulate NO production |
| title_sort |
Alkaloids from Prosopis juliflora leaves induce glial activation, cytotoxicity and stimulate NO production |
| author |
Silva, Andre Mario Mendes da |
| author_facet |
Silva, Andre Mario Mendes da Silva, Ana Rita Pinheiro, Alexandre Moraes Freitas, Sandra Regina Villas Bôas de Silva, Victor Diogenes Amaral da Souza, Cleide dos Santos Hughes, Juliana Bentes El-Bachá, Ramon dos Santos Costa, Maria de Fátima Dias Velozo, Eudes da Silva Tardy, Marcienne Bloch Costa, Silvia Lima |
| author_role |
author |
| author2 |
Silva, Ana Rita Pinheiro, Alexandre Moraes Freitas, Sandra Regina Villas Bôas de Silva, Victor Diogenes Amaral da Souza, Cleide dos Santos Hughes, Juliana Bentes El-Bachá, Ramon dos Santos Costa, Maria de Fátima Dias Velozo, Eudes da Silva Tardy, Marcienne Bloch Costa, Silvia Lima |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Andre Mario Mendes da Silva, Ana Rita Pinheiro, Alexandre Moraes Freitas, Sandra Regina Villas Bôas de Silva, Victor Diogenes Amaral da Souza, Cleide dos Santos Hughes, Juliana Bentes El-Bachá, Ramon dos Santos Costa, Maria de Fátima Dias Velozo, Eudes da Silva Tardy, Marcienne Bloch Costa, Silvia Lima Silva, Andre Mario Mendes da Silva, Ana Rita Pinheiro, Alexandre Moraes Freitas, Sandra Regina Villas Bôas de Silva, Victor Diogenes Amaral da Souza, Cleide dos Santos Hughes, Juliana Bentes El-Bachá, Ramon dos Santos Costa, Maria de Fátima Dias Velozo, Eudes da Silva Tardy, Marcienne Bloch Costa, Silvia Lima |
| dc.subject.por.fl_str_mv |
Prosopis juliflora Alkaloids Astrocytes Microglia GFAP Nitric oxide |
| topic |
Prosopis juliflora Alkaloids Astrocytes Microglia GFAP Nitric oxide |
| description |
p.601–614 |
| publishDate |
2007 |
| dc.date.issued.fl_str_mv |
2007 |
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2012-01-25T11:59:51Z |
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2012-01-25T11:59:51Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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http://www.repositorio.ufba.br/ri/handle/ri/5245 |
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0041-0101 |
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v. 49. |
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0041-0101 v. 49. |
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http://www.repositorio.ufba.br/ri/handle/ri/5245 |
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eng |
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