Effects of freezing on bone histological morphology

Detalhes bibliográficos
Autor(a) principal: Andrade, Miguel Gustavo Setúbal
Data de Publicação: 2008
Outros Autores: Sá, Camila Neves, Marchionni, Antônio Márcio Teixeira, Bittencourt, Thereza Cristina Borio dos Santos Calmon de, Sadigursky, Moysés
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/13701
Resumo: Texto completo: acesso restrito. p. 279-287
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spelling Andrade, Miguel Gustavo SetúbalSá, Camila NevesMarchionni, Antônio Márcio TeixeiraBittencourt, Thereza Cristina Borio dos Santos Calmon deSadigursky, MoysésAndrade, Miguel Gustavo SetúbalSá, Camila NevesMarchionni, Antônio Márcio TeixeiraBittencourt, Thereza Cristina Borio dos Santos Calmon deSadigursky, Moysés2013-11-18T19:53:27Z20081389-9333http://repositorio.ufba.br/ri/handle/ri/13701v. 9, n. 4Texto completo: acesso restrito. p. 279-287Allograft bone has been widely used for reconstruction of different portions of the skeleton. The fragment of bone harvested must be kept under low temperatures. The cryopreservation also contributes to decrease the antigenic potential of the tissue. Although this technique is considered safe, there is little information about the morphological modifications that the medullary and cortical portions of bone suffer after freezing. Hence, the aim of this study was to investigate the morphology of bone tissue after freezing under different temperatures and periods. Twelve rabbits were used to analyze the effects of two temperatures, −20°C and −70°C, during four periods of time: 30, 60, 90, 120 days. Tissues were analyzed by HE, picro-sirius stains and also by Feulgen’s reaction, through qualitative and morphometric ways, considering the area occupied by cells and nuclei, medullary and cortical portions, as well as by collagen expression at cortical. The differences among the treatments were analyzed by Tukey´s test, at 5% significance level. Bone freezing increased cellular and nuclear areas at cancellous bone and diminished nuclear area at the cortical bone. Cortical bone collagen suffered denaturation proportionally to temperature decrease and to freezing duration. These alterations compromised the morphology of tissues after 90 or 120 days of freezing at the temperature of −70°C. Cells necrosed during freezing, contributing to reduce bone antigenicity.Submitted by Suelen Reis (suziy.ellen@gmail.com) on 2013-11-18T11:58:38Z No. of bitstreams: 1 art%3A10.1007%2Fs10561-008-9065-4.pdf: 483937 bytes, checksum: 2955ea8bba8e42feade264f3b528b1d2 (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2013-11-18T19:53:27Z (GMT) No. of bitstreams: 1 art%3A10.1007%2Fs10561-008-9065-4.pdf: 483937 bytes, checksum: 2955ea8bba8e42feade264f3b528b1d2 (MD5)Made available in DSpace on 2013-11-18T19:53:27Z (GMT). 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dc.title.pt_BR.fl_str_mv Effects of freezing on bone histological morphology
dc.title.alternative.pt_BR.fl_str_mv Cell and Tissue Banking
title Effects of freezing on bone histological morphology
spellingShingle Effects of freezing on bone histological morphology
Andrade, Miguel Gustavo Setúbal
Bone transplantation
Freezing
Transplantation
Immunology
Necrosis
title_short Effects of freezing on bone histological morphology
title_full Effects of freezing on bone histological morphology
title_fullStr Effects of freezing on bone histological morphology
title_full_unstemmed Effects of freezing on bone histological morphology
title_sort Effects of freezing on bone histological morphology
author Andrade, Miguel Gustavo Setúbal
author_facet Andrade, Miguel Gustavo Setúbal
Sá, Camila Neves
Marchionni, Antônio Márcio Teixeira
Bittencourt, Thereza Cristina Borio dos Santos Calmon de
Sadigursky, Moysés
author_role author
author2 Sá, Camila Neves
Marchionni, Antônio Márcio Teixeira
Bittencourt, Thereza Cristina Borio dos Santos Calmon de
Sadigursky, Moysés
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Andrade, Miguel Gustavo Setúbal
Sá, Camila Neves
Marchionni, Antônio Márcio Teixeira
Bittencourt, Thereza Cristina Borio dos Santos Calmon de
Sadigursky, Moysés
Andrade, Miguel Gustavo Setúbal
Sá, Camila Neves
Marchionni, Antônio Márcio Teixeira
Bittencourt, Thereza Cristina Borio dos Santos Calmon de
Sadigursky, Moysés
dc.subject.por.fl_str_mv Bone transplantation
Freezing
Transplantation
Immunology
Necrosis
topic Bone transplantation
Freezing
Transplantation
Immunology
Necrosis
description Texto completo: acesso restrito. p. 279-287
publishDate 2008
dc.date.issued.fl_str_mv 2008
dc.date.accessioned.fl_str_mv 2013-11-18T19:53:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/13701
dc.identifier.issn.none.fl_str_mv 1389-9333
dc.identifier.number.pt_BR.fl_str_mv v. 9, n. 4
identifier_str_mv 1389-9333
v. 9, n. 4
url http://repositorio.ufba.br/ri/handle/ri/13701
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1007/s10561-008-9065-4
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collection Repositório Institucional da UFBA
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