Azurocidin, a Natural Antibiotic from Human Neutrophils: Expression, Antimicrobial Activity, and Secretion

Detalhes bibliográficos
Autor(a) principal: Almeida, Roque Pacheco de
Data de Publicação: 1996
Outros Autores: Vanet, Anne, Witko-Sarsat, Veronique, Melchior, Maxine, McCabe, Denise, Gabay, Joelle E.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/13682
Resumo: Texto completo. Acesso restrito. p. 355–366
id UFBA-2_a7610296b956e15cc80a00f6c4658f62
oai_identifier_str oai:repositorio.ufba.br:ri/13682
network_acronym_str UFBA-2
network_name_str Repositório Institucional da UFBA
repository_id_str 1932
spelling Almeida, Roque Pacheco deVanet, AnneWitko-Sarsat, VeroniqueMelchior, MaxineMcCabe, DeniseGabay, Joelle E.Almeida, Roque Pacheco deVanet, AnneWitko-Sarsat, VeroniqueMelchior, MaxineMcCabe, DeniseGabay, Joelle E.2013-11-16T13:15:44Z19961046-5928http://repositorio.ufba.br/ri/handle/ri/13682v. 7, n. 4Texto completo. Acesso restrito. p. 355–366The azurophil granules of human PMN contain four antibiotic proteins, the serprocidins, which have extensive homology to one another and to serine proteases. Azurocidin, a member of this family, is a 29-kDa glycoprotein with broad spectrum antimicrobial activity and chemotactic activity toward monocytes. Insect cells transfected with a baculovirus vector carrying azurocidin cDNA produced a recombinant azurocidin protein. We purified the recombinant azurocidin protein from the culture medium of the infected cells and showed that it retained the antimicrobial activity of the native neutrophil-derived molecule. In addition, we present evidence that a 49-amino-acid region of the recombinant azurocidin protein is required for its secretion from insect cells.Submitted by Santiago Fabio (fabio.ssantiago@hotmail.com) on 2013-09-09T18:03:41Z No. of bitstreams: 1 11111111.pdf: 374080 bytes, checksum: 95febf9cb2121d9cf26a5318582ba2d8 (MD5)Approved for entry into archive by Rodrigo Meirelles (rodrigomei@ufba.br) on 2013-11-16T13:15:44Z (GMT) No. of bitstreams: 1 11111111.pdf: 374080 bytes, checksum: 95febf9cb2121d9cf26a5318582ba2d8 (MD5)Made available in DSpace on 2013-11-16T13:15:44Z (GMT). No. of bitstreams: 1 11111111.pdf: 374080 bytes, checksum: 95febf9cb2121d9cf26a5318582ba2d8 (MD5) Previous issue date: 1996SalvadorProtein Expression and Purificationhttp://ac.els-cdn.com.ez10.periodicos.capes.gov.br/S1046592896900534/1-s2.0-S1046592896900534-main.pdf?_tid=d9c95dfc-1977-11e3-9ca4-00000aab0f6b&acdnat=1378749009_93dac86784908821d1d8f4822de2bae0reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBAAzurocidin, a Natural Antibiotic from Human Neutrophils: Expression, Antimicrobial Activity, and SecretionProtein Expression and Purificationinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINAL11111111.pdf11111111.pdfapplication/pdf374080https://repositorio.ufba.br/bitstream/ri/13682/1/11111111.pdf95febf9cb2121d9cf26a5318582ba2d8MD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/13682/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT11111111.pdf.txt11111111.pdf.txtExtracted texttext/plain50154https://repositorio.ufba.br/bitstream/ri/13682/3/11111111.pdf.txt5ebfba86f9f6cbf8ae2df958aa343f1cMD53ri/136822022-08-08 13:17:26.892oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-08-08T16:17:26Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Azurocidin, a Natural Antibiotic from Human Neutrophils: Expression, Antimicrobial Activity, and Secretion
dc.title.alternative.pt_BR.fl_str_mv Protein Expression and Purification
title Azurocidin, a Natural Antibiotic from Human Neutrophils: Expression, Antimicrobial Activity, and Secretion
spellingShingle Azurocidin, a Natural Antibiotic from Human Neutrophils: Expression, Antimicrobial Activity, and Secretion
Almeida, Roque Pacheco de
title_short Azurocidin, a Natural Antibiotic from Human Neutrophils: Expression, Antimicrobial Activity, and Secretion
title_full Azurocidin, a Natural Antibiotic from Human Neutrophils: Expression, Antimicrobial Activity, and Secretion
title_fullStr Azurocidin, a Natural Antibiotic from Human Neutrophils: Expression, Antimicrobial Activity, and Secretion
title_full_unstemmed Azurocidin, a Natural Antibiotic from Human Neutrophils: Expression, Antimicrobial Activity, and Secretion
title_sort Azurocidin, a Natural Antibiotic from Human Neutrophils: Expression, Antimicrobial Activity, and Secretion
author Almeida, Roque Pacheco de
author_facet Almeida, Roque Pacheco de
Vanet, Anne
Witko-Sarsat, Veronique
Melchior, Maxine
McCabe, Denise
Gabay, Joelle E.
author_role author
author2 Vanet, Anne
Witko-Sarsat, Veronique
Melchior, Maxine
McCabe, Denise
Gabay, Joelle E.
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida, Roque Pacheco de
Vanet, Anne
Witko-Sarsat, Veronique
Melchior, Maxine
McCabe, Denise
Gabay, Joelle E.
Almeida, Roque Pacheco de
Vanet, Anne
Witko-Sarsat, Veronique
Melchior, Maxine
McCabe, Denise
Gabay, Joelle E.
description Texto completo. Acesso restrito. p. 355–366
publishDate 1996
dc.date.issued.fl_str_mv 1996
dc.date.accessioned.fl_str_mv 2013-11-16T13:15:44Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/13682
dc.identifier.issn.none.fl_str_mv 1046-5928
dc.identifier.number.pt_BR.fl_str_mv v. 7, n. 4
identifier_str_mv 1046-5928
v. 7, n. 4
url http://repositorio.ufba.br/ri/handle/ri/13682
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Protein Expression and Purification
publisher.none.fl_str_mv Protein Expression and Purification
dc.source.pt_BR.fl_str_mv http://ac.els-cdn.com.ez10.periodicos.capes.gov.br/S1046592896900534/1-s2.0-S1046592896900534-main.pdf?_tid=d9c95dfc-1977-11e3-9ca4-00000aab0f6b&acdnat=1378749009_93dac86784908821d1d8f4822de2bae0
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
bitstream.url.fl_str_mv https://repositorio.ufba.br/bitstream/ri/13682/1/11111111.pdf
https://repositorio.ufba.br/bitstream/ri/13682/2/license.txt
https://repositorio.ufba.br/bitstream/ri/13682/3/11111111.pdf.txt
bitstream.checksum.fl_str_mv 95febf9cb2121d9cf26a5318582ba2d8
1b89a9a0548218172d7c829f87a0eab9
5ebfba86f9f6cbf8ae2df958aa343f1c
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)
repository.mail.fl_str_mv
_version_ 1808459458211217408

Registros relacionados