Photodynamic antimicrobial chemotherapy (PACT) using phenothiazine derivatives as photosensitizers against Leishmania braziliensis

Detalhes bibliográficos
Autor(a) principal: Barbosa, Artur Felipe Santos
Data de Publicação: 2012
Outros Autores: Sangiorgi, Bruno Braga, Galdino, Suely L., Barral-Netto, Manoel, Pitta, Ivan R., Pinheiro, Antonio Luiz Barbosa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://repositorio.ufba.br/ri/handle/ri/16439
Resumo: Texto completo: acesso restrito. p. 850–855
id UFBA-2_a874ab4185d767eba85096ad993360a5
oai_identifier_str oai:repositorio.ufba.br:ri/16439
network_acronym_str UFBA-2
network_name_str Repositório Institucional da UFBA
repository_id_str 1932
spelling Barbosa, Artur Felipe SantosSangiorgi, Bruno BragaGaldino, Suely L.Barral-Netto, ManoelPitta, Ivan R.Pinheiro, Antonio Luiz BarbosaBarbosa, Artur Felipe SantosSangiorgi, Bruno BragaGaldino, Suely L.Barral-Netto, ManoelPitta, Ivan R.Pinheiro, Antonio Luiz Barbosa2014-10-21T16:07:35Z20120196-8092http://repositorio.ufba.br/ri/handle/ri/16439v. 44, n. 10Texto completo: acesso restrito. p. 850–855Background and Objective: Cutaneous and mucocutaneous leishmaniasis are diseases characterized by skin or mucosal manifestations. In the new world, Leishmania braziliensis is the main etiological agent of cutaneous leishmaniasis, condition that may evolve to the mucocutaneous form. The therapeutic arsenal routinely employed to treat infected patients is unsatisfactory, especially for pentavalent antimonials, treatment recommended by the WHO, as they are often highly toxic, poorly tolerated and of variable effectiveness. This work aimed to evaluate in vitro the effectiveness of photodynamic antimicrobial chemotherapy as a new approach for the treatment of leishmaniasis. Materials and Methods: A laser (λ = 660 nm, 40 mW, 4.2 J/cm2, and 8.4 J/cm2, CW) associated to phenothiazine's derivatives (5 and 10 µg/ml, toluidine blue O, methylene blue, or phenothiazine) on the promastigote forms of L. braziliensis in a single session. Samples were removed and analyzed in a hemocytometer 72 hours after PACT and viability of the parasites was assessed in quadruplicates. Results: An important decrease in the number of viable parasites on all treated groups in comparison to their controls was observed as all tested compounds lead to significant parasite lethality being the highest lethality achieved with 10 µg/ml of TBO. No lethality was observed on groups treated with laser or with any of the compounds separately. Conclusions: TBO presented higher parasite lethality in comparison to MB with impressive reduction from 1 hour to 5 minutes of pre-incubation time. Lasers Surg. Med. 44: 850–855, 2012. © 2012 Wiley Periodicals, Inc.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2014-04-24T13:52:59Z No. of bitstreams: 1 Artur F. Barbosa.pdf: 86697 bytes, checksum: 4a92282542739db1e21fdff38552ed8d (MD5)Approved for entry into archive by Flávia Ferreira (flaviaccf@yahoo.com.br) on 2014-10-21T16:07:35Z (GMT) No. of bitstreams: 1 Artur F. Barbosa.pdf: 86697 bytes, checksum: 4a92282542739db1e21fdff38552ed8d (MD5)Made available in DSpace on 2014-10-21T16:07:35Z (GMT). No. of bitstreams: 1 Artur F. Barbosa.pdf: 86697 bytes, checksum: 4a92282542739db1e21fdff38552ed8d (MD5) Previous issue date: 2012http://dx.doi.org/ 10.1002/lsm.22099reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBALaser-therapyLeishmaniasisMethylene blueToluidine blue OTreatmentPhotodynamic antimicrobial chemotherapy (PACT) using phenothiazine derivatives as photosensitizers against Leishmania braziliensisLasers in Surgery and Medicineinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article10000-01-01info:eu-repo/semantics/openAccessengORIGINALArtur F. Barbosa.pdfArtur F. Barbosa.pdfapplication/pdf86697https://repositorio.ufba.br/bitstream/ri/16439/1/Artur%20F.%20Barbosa.pdf4a92282542739db1e21fdff38552ed8dMD51LICENSElicense.txtlicense.txttext/plain1345https://repositorio.ufba.br/bitstream/ri/16439/2/license.txt0d4b811ef71182510d2015daa7c8a900MD52TEXTArtur F. Barbosa.pdf.txtArtur F. Barbosa.pdf.txtExtracted texttext/plain33467https://repositorio.ufba.br/bitstream/ri/16439/3/Artur%20F.%20Barbosa.pdf.txt54c5670f2cf57f2dcaa36bcc4192f7daMD53ri/164392022-09-29 10:26:47.25oai:repositorio.ufba.br:ri/16439VGVybW8gZGUgTGljZW4/YSwgbj9vIGV4Y2x1c2l2bywgcGFyYSBvIGRlcD9zaXRvIG5vIFJlcG9zaXQ/cmlvIEluc3RpdHVjaW9uYWwgZGEgVUZCQS4KCiBQZWxvIHByb2Nlc3NvIGRlIHN1Ym1pc3M/byBkZSBkb2N1bWVudG9zLCBvIGF1dG9yIG91IHNldSByZXByZXNlbnRhbnRlIGxlZ2FsLCBhbyBhY2VpdGFyIAplc3NlIHRlcm1vIGRlIGxpY2VuP2EsIGNvbmNlZGUgYW8gUmVwb3NpdD9yaW8gSW5zdGl0dWNpb25hbCBkYSBVbml2ZXJzaWRhZGUgRmVkZXJhbCBkYSBCYWhpYSAKbyBkaXJlaXRvIGRlIG1hbnRlciB1bWEgYz9waWEgZW0gc2V1IHJlcG9zaXQ/cmlvIGNvbSBhIGZpbmFsaWRhZGUsIHByaW1laXJhLCBkZSBwcmVzZXJ2YT8/by4gCkVzc2VzIHRlcm1vcywgbj9vIGV4Y2x1c2l2b3MsIG1hbnQ/bSBvcyBkaXJlaXRvcyBkZSBhdXRvci9jb3B5cmlnaHQsIG1hcyBlbnRlbmRlIG8gZG9jdW1lbnRvIApjb21vIHBhcnRlIGRvIGFjZXJ2byBpbnRlbGVjdHVhbCBkZXNzYSBVbml2ZXJzaWRhZGUuCgogUGFyYSBvcyBkb2N1bWVudG9zIHB1YmxpY2Fkb3MgY29tIHJlcGFzc2UgZGUgZGlyZWl0b3MgZGUgZGlzdHJpYnVpPz9vLCBlc3NlIHRlcm1vIGRlIGxpY2VuP2EgCmVudGVuZGUgcXVlOgoKIE1hbnRlbmRvIG9zIGRpcmVpdG9zIGF1dG9yYWlzLCByZXBhc3NhZG9zIGEgdGVyY2Vpcm9zLCBlbSBjYXNvIGRlIHB1YmxpY2E/P2VzLCBvIHJlcG9zaXQ/cmlvCnBvZGUgcmVzdHJpbmdpciBvIGFjZXNzbyBhbyB0ZXh0byBpbnRlZ3JhbCwgbWFzIGxpYmVyYSBhcyBpbmZvcm1hPz9lcyBzb2JyZSBvIGRvY3VtZW50bwooTWV0YWRhZG9zIGVzY3JpdGl2b3MpLgoKIERlc3RhIGZvcm1hLCBhdGVuZGVuZG8gYW9zIGFuc2Vpb3MgZGVzc2EgdW5pdmVyc2lkYWRlIGVtIG1hbnRlciBzdWEgcHJvZHU/P28gY2llbnQ/ZmljYSBjb20gCmFzIHJlc3RyaT8/ZXMgaW1wb3N0YXMgcGVsb3MgZWRpdG9yZXMgZGUgcGVyaT9kaWNvcy4KCiBQYXJhIGFzIHB1YmxpY2E/P2VzIHNlbSBpbmljaWF0aXZhcyBxdWUgc2VndWVtIGEgcG9sP3RpY2EgZGUgQWNlc3NvIEFiZXJ0bywgb3MgZGVwP3NpdG9zIApjb21wdWxzP3Jpb3MgbmVzc2UgcmVwb3NpdD9yaW8gbWFudD9tIG9zIGRpcmVpdG9zIGF1dG9yYWlzLCBtYXMgbWFudD9tIGFjZXNzbyBpcnJlc3RyaXRvIAphbyBtZXRhZGFkb3MgZSB0ZXh0byBjb21wbGV0by4gQXNzaW0sIGEgYWNlaXRhPz9vIGRlc3NlIHRlcm1vIG4/byBuZWNlc3NpdGEgZGUgY29uc2VudGltZW50bwogcG9yIHBhcnRlIGRlIGF1dG9yZXMvZGV0ZW50b3JlcyBkb3MgZGlyZWl0b3MsIHBvciBlc3RhcmVtIGVtIGluaWNpYXRpdmFzIGRlIGFjZXNzbyBhYmVydG8uCg==Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-09-29T13:26:47Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false
dc.title.pt_BR.fl_str_mv Photodynamic antimicrobial chemotherapy (PACT) using phenothiazine derivatives as photosensitizers against Leishmania braziliensis
dc.title.alternative.pt_BR.fl_str_mv Lasers in Surgery and Medicine
title Photodynamic antimicrobial chemotherapy (PACT) using phenothiazine derivatives as photosensitizers against Leishmania braziliensis
spellingShingle Photodynamic antimicrobial chemotherapy (PACT) using phenothiazine derivatives as photosensitizers against Leishmania braziliensis
Barbosa, Artur Felipe Santos
Laser-therapy
Leishmaniasis
Methylene blue
Toluidine blue O
Treatment
title_short Photodynamic antimicrobial chemotherapy (PACT) using phenothiazine derivatives as photosensitizers against Leishmania braziliensis
title_full Photodynamic antimicrobial chemotherapy (PACT) using phenothiazine derivatives as photosensitizers against Leishmania braziliensis
title_fullStr Photodynamic antimicrobial chemotherapy (PACT) using phenothiazine derivatives as photosensitizers against Leishmania braziliensis
title_full_unstemmed Photodynamic antimicrobial chemotherapy (PACT) using phenothiazine derivatives as photosensitizers against Leishmania braziliensis
title_sort Photodynamic antimicrobial chemotherapy (PACT) using phenothiazine derivatives as photosensitizers against Leishmania braziliensis
author Barbosa, Artur Felipe Santos
author_facet Barbosa, Artur Felipe Santos
Sangiorgi, Bruno Braga
Galdino, Suely L.
Barral-Netto, Manoel
Pitta, Ivan R.
Pinheiro, Antonio Luiz Barbosa
author_role author
author2 Sangiorgi, Bruno Braga
Galdino, Suely L.
Barral-Netto, Manoel
Pitta, Ivan R.
Pinheiro, Antonio Luiz Barbosa
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Barbosa, Artur Felipe Santos
Sangiorgi, Bruno Braga
Galdino, Suely L.
Barral-Netto, Manoel
Pitta, Ivan R.
Pinheiro, Antonio Luiz Barbosa
Barbosa, Artur Felipe Santos
Sangiorgi, Bruno Braga
Galdino, Suely L.
Barral-Netto, Manoel
Pitta, Ivan R.
Pinheiro, Antonio Luiz Barbosa
dc.subject.por.fl_str_mv Laser-therapy
Leishmaniasis
Methylene blue
Toluidine blue O
Treatment
topic Laser-therapy
Leishmaniasis
Methylene blue
Toluidine blue O
Treatment
description Texto completo: acesso restrito. p. 850–855
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-10-21T16:07:35Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://repositorio.ufba.br/ri/handle/ri/16439
dc.identifier.issn.none.fl_str_mv 0196-8092
dc.identifier.number.pt_BR.fl_str_mv v. 44, n. 10
identifier_str_mv 0196-8092
v. 44, n. 10
url http://repositorio.ufba.br/ri/handle/ri/16439
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.source.pt_BR.fl_str_mv http://dx.doi.org/ 10.1002/lsm.22099
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
instname:Universidade Federal da Bahia (UFBA)
instacron:UFBA
instname_str Universidade Federal da Bahia (UFBA)
instacron_str UFBA
institution UFBA
reponame_str Repositório Institucional da UFBA
collection Repositório Institucional da UFBA
bitstream.url.fl_str_mv https://repositorio.ufba.br/bitstream/ri/16439/1/Artur%20F.%20Barbosa.pdf
https://repositorio.ufba.br/bitstream/ri/16439/2/license.txt
https://repositorio.ufba.br/bitstream/ri/16439/3/Artur%20F.%20Barbosa.pdf.txt
bitstream.checksum.fl_str_mv 4a92282542739db1e21fdff38552ed8d
0d4b811ef71182510d2015daa7c8a900
54c5670f2cf57f2dcaa36bcc4192f7da
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)
repository.mail.fl_str_mv
_version_ 1801502535628881920

Registros relacionados