Serologic immunoreactivity to Neospora caninum antigens in dogs determined by indirect immunofluorescence, western blotting and dot-ELISA

Detalhes bibliográficos
Autor(a) principal: Pinheiro, A. M.
Data de Publicação: 2005
Outros Autores: Costa, Maria de Fátima Dias, Paule, B., Vale, V., Ribeiro, M., Nascimento, I., Schaer, Robert Eduard, Almeida, Maria Angela Ornelas de, Nascimento, Roberto José Meyer, Freire, S. M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da UFBA
Texto Completo: http://www.repositorio.ufba.br/ri/handle/ri/6478
Resumo: Trabalho completo: acesso restrito, p. 73–79
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spelling Pinheiro, A. M.Costa, Maria de Fátima DiasPaule, B.Vale, V.Ribeiro, M.Nascimento, I.Schaer, Robert EduardAlmeida, Maria Angela Ornelas deNascimento, Roberto José MeyerFreire, S. M.Pinheiro, A. M.Costa, Maria de Fátima DiasPaule, B.Vale, V.Ribeiro, M.Nascimento, I.Schaer, Robert EduardAlmeida, Maria Angela Ornelas deNascimento, Roberto José MeyerFreire, S. M.2012-07-20T14:50:59Z2005-06-100304-4017http://www.repositorio.ufba.br/ri/handle/ri/6478v. 130, n. 1–2Trabalho completo: acesso restrito, p. 73–79Neospora caninum, is a coccidian protozoan known as a major cause of bovine abortion and canine neuropathies. The aim of the present study was to develop a reliable and quick test to detect antibodies to N. caninum in dog sera. Sixty-five serum samples from dogs, including 35 positive and 30 negative for N. caninum antibodies were used for standardization of the test. In parallel, immunoreactivity of the sera to Toxoplasma gondii antigens was investigated using a passive agglutination test. A dot-ELISA test, using soluble extract of N. caninum tachyzoites on nitrocellulose ester membranes, was developed and standardized. SDS-PAGE and complementary analysis of reactivity by Western blotting were used for the characterization of the immunoreactive fractions of all tested sera. The sensitivity and specificity of the dot-ELISA were 94 and 73%, respectively, compared to IFAT at a cut-off of 1:50, and 87 and 100% compared to IFAT at a cut-off of 1:25. Among the sera that tested positively for both IFAT and dot-ELISA, only 8.6% were reactive to T. gondii. The most immunoreactive fractions in Western blots were the 14-, 33-, 42- and 55 kDa bands, with percentages of 42, 60, 42 and 37%, respectively. The 60 kDa band showed a non-specific reaction in 43% of neosporosis-negative animals by both dot-ELISA and IFAT. These results indicate that the dot-ELISA using N. caninum antigen present good sensitivity and specificity, and might be used as a screening test to detect antibodies to N. caninum in dogs.Submitted by Bruna Lessa (lessbruna@gmail.com) on 2012-07-20T14:50:59Z No. of bitstreams: 1 (129).pdf: 229000 bytes, checksum: e7aef75daf6a139f35e0e57bc968c9db (MD5)Made available in DSpace on 2012-07-20T14:50:59Z (GMT). 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dc.title.pt_BR.fl_str_mv Serologic immunoreactivity to Neospora caninum antigens in dogs determined by indirect immunofluorescence, western blotting and dot-ELISA
dc.title.alternative.pt_BR.fl_str_mv Veterinary Parasitology
title Serologic immunoreactivity to Neospora caninum antigens in dogs determined by indirect immunofluorescence, western blotting and dot-ELISA
spellingShingle Serologic immunoreactivity to Neospora caninum antigens in dogs determined by indirect immunofluorescence, western blotting and dot-ELISA
Pinheiro, A. M.
Neospora caninum
Immunodiagnosis
dot-ELISA
Dogs
title_short Serologic immunoreactivity to Neospora caninum antigens in dogs determined by indirect immunofluorescence, western blotting and dot-ELISA
title_full Serologic immunoreactivity to Neospora caninum antigens in dogs determined by indirect immunofluorescence, western blotting and dot-ELISA
title_fullStr Serologic immunoreactivity to Neospora caninum antigens in dogs determined by indirect immunofluorescence, western blotting and dot-ELISA
title_full_unstemmed Serologic immunoreactivity to Neospora caninum antigens in dogs determined by indirect immunofluorescence, western blotting and dot-ELISA
title_sort Serologic immunoreactivity to Neospora caninum antigens in dogs determined by indirect immunofluorescence, western blotting and dot-ELISA
author Pinheiro, A. M.
author_facet Pinheiro, A. M.
Costa, Maria de Fátima Dias
Paule, B.
Vale, V.
Ribeiro, M.
Nascimento, I.
Schaer, Robert Eduard
Almeida, Maria Angela Ornelas de
Nascimento, Roberto José Meyer
Freire, S. M.
author_role author
author2 Costa, Maria de Fátima Dias
Paule, B.
Vale, V.
Ribeiro, M.
Nascimento, I.
Schaer, Robert Eduard
Almeida, Maria Angela Ornelas de
Nascimento, Roberto José Meyer
Freire, S. M.
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pinheiro, A. M.
Costa, Maria de Fátima Dias
Paule, B.
Vale, V.
Ribeiro, M.
Nascimento, I.
Schaer, Robert Eduard
Almeida, Maria Angela Ornelas de
Nascimento, Roberto José Meyer
Freire, S. M.
Pinheiro, A. M.
Costa, Maria de Fátima Dias
Paule, B.
Vale, V.
Ribeiro, M.
Nascimento, I.
Schaer, Robert Eduard
Almeida, Maria Angela Ornelas de
Nascimento, Roberto José Meyer
Freire, S. M.
dc.subject.por.fl_str_mv Neospora caninum
Immunodiagnosis
dot-ELISA
Dogs
topic Neospora caninum
Immunodiagnosis
dot-ELISA
Dogs
description Trabalho completo: acesso restrito, p. 73–79
publishDate 2005
dc.date.issued.fl_str_mv 2005-06-10
dc.date.accessioned.fl_str_mv 2012-07-20T14:50:59Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.repositorio.ufba.br/ri/handle/ri/6478
dc.identifier.issn.none.fl_str_mv 0304-4017
dc.identifier.number.pt_BR.fl_str_mv v. 130, n. 1–2
identifier_str_mv 0304-4017
v. 130, n. 1–2
url http://www.repositorio.ufba.br/ri/handle/ri/6478
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.pt_BR.fl_str_mv http://dx.doi.org/10.1016/j.vetpar.2005.03.018
dc.source.none.fl_str_mv reponame:Repositório Institucional da UFBA
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collection Repositório Institucional da UFBA
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