Differential effects of antigens from L. braziliensis isolates from disseminated and cutaneous leishmaniasis on in vitro cytokine production
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2006 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da UFBA |
| Texto Completo: | http://www.repositorio.ufba.br/ri/handle/ri/12760 |
Resumo: | p.1-6 |
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Schriefer, Nicolaus Albert BorgesLeopoldo, Paulo T. G.Machado, Paulo Roberto LimaAlmeida, Roque Pacheco deGiudice, AngelaJesus, Amélia Ribeiro deGuimarães, Luiz HenriqueBacellar, Maria Olívia Amado RamosCarvalho Filho, Edgar Marcelino deSchriefer, Nicolaus Albert BorgesLeopoldo, Paulo T. G.Machado, Paulo Roberto LimaAlmeida, Roque Pacheco deGiudice, AngelaJesus, Amélia Ribeiro deGuimarães, Luiz HenriqueBacellar, Maria Olívia Amado RamosCarvalho Filho, Edgar Marcelino de2013-08-27T17:37:57Z2013-08-27T17:37:57Z20061471-2334http://www.repositorio.ufba.br/ri/handle/ri/12760v.6 n. 75p.1-6Background: Disseminated leishmaniasis is an emerging infectious disease, mostly due to L. braziliensis, which has clinical and histopathological features distinct from cutaneous leishmaniasis. Methods: In the current study we evaluated the in vitro production of the cytokines IFN-γ, TNF-α, IL-5 and IL-10 by peripheral blood mononuclear cells (PBMC) from 15 disseminated leishmaniasis and 24 cutaneous leishmaniasis patients upon stimulation with L. braziliensis antigens genotyped as disseminated leishmaniasis or cutaneous leishmaniasis isolates. Results: Regardless of the source of L. braziliensis antigens, PBMC from cutaneous leishmaniasis patients produced significantly higher IFN-γ than PBMC from disseminated leishmaniasis patients. Levels of TNF-α by PBMC from cutaneous leishmaniasis patients were significantly higher than disseminated leishmaniasis patients only when stimulated by genotyped cutaneous leishmaniasis antigens. The levels of IL-5 and IL-10 production by PBMC were very low and similar in PBMCs from both disseminated leishmaniasis and cutaneous leishmaniasis patients. The immune response of each patient evaluated by the two L. braziliensis antigens was assessed in a paired analysis in which we showed that L. braziliensis genotyped as disseminated leishmaniasis isolate was more potent than L. braziliensis genotyped as cutaneous leishmaniasis isolate in triggering IFN-γ and TNF-α production in both diseases and IL-5 only in cutaneous leishmaniasis patients. Conclusion: This study provides evidence that antigens prepared from genotypically distinct strains of L. braziliensis induce different degrees of immune response. It also indicates that both parasite and host play a role in the outcome of L. braziliensis infection.Submitted by Edileide Reis (leyde-landy@hotmail.com) on 2013-08-27T11:36:10Z No. of bitstreams: 1 1471-2334-6-75.pdf: 289112 bytes, checksum: 456a013c7aad83ff1a9b0fa90711a95d (MD5)Approved for entry into archive by Patricia Barroso(pbarroso@ufba.br) on 2013-08-27T17:37:57Z (GMT) No. of bitstreams: 1 1471-2334-6-75.pdf: 289112 bytes, checksum: 456a013c7aad83ff1a9b0fa90711a95d (MD5)Made available in DSpace on 2013-08-27T17:37:57Z (GMT). No. of bitstreams: 1 1471-2334-6-75.pdf: 289112 bytes, checksum: 456a013c7aad83ff1a9b0fa90711a95d (MD5) Previous issue date: 2006http://dx.doi.org/ doi:10.1186/1471-2334-6-75reponame:Repositório Institucional da UFBAinstname:Universidade Federal da Bahia (UFBA)instacron:UFBADifferential effects of antigens from L. braziliensis isolates from disseminated and cutaneous leishmaniasis on in vitro cytokine productionBMC Infectious Diseasesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleenginfo:eu-repo/semantics/openAccessORIGINAL1471-2334-6-75.pdf1471-2334-6-75.pdfapplication/pdf289112https://repositorio.ufba.br/bitstream/ri/12760/1/1471-2334-6-75.pdf456a013c7aad83ff1a9b0fa90711a95dMD51LICENSElicense.txtlicense.txttext/plain1762https://repositorio.ufba.br/bitstream/ri/12760/2/license.txt1b89a9a0548218172d7c829f87a0eab9MD52TEXT1471-2334-6-75.pdf.txt1471-2334-6-75.pdf.txtExtracted texttext/plain27693https://repositorio.ufba.br/bitstream/ri/12760/3/1471-2334-6-75.pdf.txt530fc52ff9b14b15677ecb6f43c2f720MD53ri/127602022-07-05 14:02:49.671oai:repositorio.ufba.br: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Repositório InstitucionalPUBhttp://192.188.11.11:8080/oai/requestopendoar:19322022-07-05T17:02:49Repositório Institucional da UFBA - Universidade Federal da Bahia (UFBA)false |
| dc.title.pt_BR.fl_str_mv |
Differential effects of antigens from L. braziliensis isolates from disseminated and cutaneous leishmaniasis on in vitro cytokine production |
| dc.title.alternative.pt_BR.fl_str_mv |
BMC Infectious Diseases |
| title |
Differential effects of antigens from L. braziliensis isolates from disseminated and cutaneous leishmaniasis on in vitro cytokine production |
| spellingShingle |
Differential effects of antigens from L. braziliensis isolates from disseminated and cutaneous leishmaniasis on in vitro cytokine production Schriefer, Nicolaus Albert Borges |
| title_short |
Differential effects of antigens from L. braziliensis isolates from disseminated and cutaneous leishmaniasis on in vitro cytokine production |
| title_full |
Differential effects of antigens from L. braziliensis isolates from disseminated and cutaneous leishmaniasis on in vitro cytokine production |
| title_fullStr |
Differential effects of antigens from L. braziliensis isolates from disseminated and cutaneous leishmaniasis on in vitro cytokine production |
| title_full_unstemmed |
Differential effects of antigens from L. braziliensis isolates from disseminated and cutaneous leishmaniasis on in vitro cytokine production |
| title_sort |
Differential effects of antigens from L. braziliensis isolates from disseminated and cutaneous leishmaniasis on in vitro cytokine production |
| author |
Schriefer, Nicolaus Albert Borges |
| author_facet |
Schriefer, Nicolaus Albert Borges Leopoldo, Paulo T. G. Machado, Paulo Roberto Lima Almeida, Roque Pacheco de Giudice, Angela Jesus, Amélia Ribeiro de Guimarães, Luiz Henrique Bacellar, Maria Olívia Amado Ramos Carvalho Filho, Edgar Marcelino de |
| author_role |
author |
| author2 |
Leopoldo, Paulo T. G. Machado, Paulo Roberto Lima Almeida, Roque Pacheco de Giudice, Angela Jesus, Amélia Ribeiro de Guimarães, Luiz Henrique Bacellar, Maria Olívia Amado Ramos Carvalho Filho, Edgar Marcelino de |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Schriefer, Nicolaus Albert Borges Leopoldo, Paulo T. G. Machado, Paulo Roberto Lima Almeida, Roque Pacheco de Giudice, Angela Jesus, Amélia Ribeiro de Guimarães, Luiz Henrique Bacellar, Maria Olívia Amado Ramos Carvalho Filho, Edgar Marcelino de Schriefer, Nicolaus Albert Borges Leopoldo, Paulo T. G. Machado, Paulo Roberto Lima Almeida, Roque Pacheco de Giudice, Angela Jesus, Amélia Ribeiro de Guimarães, Luiz Henrique Bacellar, Maria Olívia Amado Ramos Carvalho Filho, Edgar Marcelino de |
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p.1-6 |
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2006 |
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2006 |
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2013-08-27T17:37:57Z |
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2013-08-27T17:37:57Z |
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1471-2334 |
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v.6 n. 75 |
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1471-2334 v.6 n. 75 |
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